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The map label for this gene is rutF

Identifier: 157370061

GI number: 157370061

Start: 2003182

End: 2003709

Strand: Reverse

Name: rutF

Synonym: Spro_1819

Alternate gene names: 157370061

Gene position: 2003709-2003182 (Counterclockwise)

Preceding gene: 157370062

Following gene: 157370060

Centisome position: 36.77

GC content: 59.09

Gene sequence:

>528_bases
ATGCAAAGCCAAACGCTGCTTGCCGATACGCCACTACAGTCGCCGTATGTAGAAAAACAGGACTTCCGCGACGCCATGGC
GCGCCTGGGATCGGCAGTCAATATCATCACCACCGATGGCCCTGCCGGCCGGGCCGGTTTTACCGCTTCGGCTGTCTGCA
GCGTCACCGACACGCCACCAACCTTGTTGGTGTGCCTGAACCGTTCCGCGTCGGTCTATTCGGTGTTCAAACAAAACCAG
ACGCTGTGCGTCAATACGCTGGCCGCGGAGCATGAGTCGCTGTCGAATCTATTCGGCGGTAAAACCCCGATGGAGCTGCG
ATTTTCCGCTGCACGCTGGTCTACGCTGGCGACCGGATCCCCCATCCTGCACGGTGCAGTGGTCTCGTTCGACTGCCAAA
TCGGCCAGATTGTCAGCGTCGGCACCCACGACATTTTTTTCTGCCAGGCGCTGGCGCTGACGCGTAATGACGACAGCCAC
GGTCTGGCCTATTTCGACCGGCGCTACCACTCGCTGTTGAAACAATGA

Upstream 100 bases:

>100_bases
GCGTGACCGACACCGAGCACTTTAACCGCATCCTGCTGGGTTGGCTGTTGAAAACGCAAAACGCCCAAACCCGCCTGCAA
CCCTAGCCCGGAGACACAAA

Downstream 100 bases:

>100_bases
TCGGCGCATAACCGCTCAAACACATTCACCCGCACCCTGAAATTTTGGAGATAACGATGGCGAATACCTGGTTTCCCCAA
TGGCGCATGAAACAAGGCAG

Product: flavin reductase domain-containing protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MQSQTLLADTPLQSPYVEKQDFRDAMARLGSAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRSASVYSVFKQNQ
TLCVNTLAAEHESLSNLFGGKTPMELRFSAARWSTLATGSPILHGAVVSFDCQIGQIVSVGTHDIFFCQALALTRNDDSH
GLAYFDRRYHSLLKQ

Sequences:

>Translated_175_residues
MQSQTLLADTPLQSPYVEKQDFRDAMARLGSAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRSASVYSVFKQNQ
TLCVNTLAAEHESLSNLFGGKTPMELRFSAARWSTLATGSPILHGAVVSFDCQIGQIVSVGTHDIFFCQALALTRNDDSH
GLAYFDRRYHSLLKQ
>Mature_175_residues
MQSQTLLADTPLQSPYVEKQDFRDAMARLGSAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRSASVYSVFKQNQ
TLCVNTLAAEHESLSNLFGGKTPMELRFSAARWSTLATGSPILHGAVVSFDCQIGQIVSVGTHDIFFCQALALTRNDDSH
GLAYFDRRYHSLLKQ

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily

Homologues:

Organism=Escherichia coli, GI87081819, Length=158, Percent_Identity=69.620253164557, Blast_Score=234, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTF_SERP5 (A8GCT2)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478050.1
- ProteinModelPortal:   A8GCT2
- SMR:   A8GCT2
- STRING:   A8GCT2
- GeneID:   5605393
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1819
- eggNOG:   COG1853
- HOGENOM:   HBG730363
- OMA:   NRSASVY
- ProtClustDB:   CLSK964034
- BioCyc:   SPRO399741:SPRO_1819-MONOMER
- HAMAP:   MF_00833
- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- Gene3D:   G3DSA:2.30.110.10
- SMART:   SM00903
- TIGRFAMs:   TIGR03615

Pfam domain/function: PF01613 Flavin_Reduct; SSF50475 FMN_binding

EC number: =1.5.1.29

Molecular weight: Translated: 18931; Mature: 18931

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSQTLLADTPLQSPYVEKQDFRDAMARLGSAVNIITTDGPAGRAGFTASAVCSVTDTPP
CCCCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCC
TLLVCLNRSASVYSVFKQNQTLCVNTLAAEHESLSNLFGGKTPMELRFSAARWSTLATGS
EEEEEECCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCC
PILHGAVVSFDCQIGQIVSVGTHDIFFCQALALTRNDDSHGLAYFDRRYHSLLKQ
CCEECEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQSQTLLADTPLQSPYVEKQDFRDAMARLGSAVNIITTDGPAGRAGFTASAVCSVTDTPP
CCCCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCC
TLLVCLNRSASVYSVFKQNQTLCVNTLAAEHESLSNLFGGKTPMELRFSAARWSTLATGS
EEEEEECCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCC
PILHGAVVSFDCQIGQIVSVGTHDIFFCQALALTRNDDSHGLAYFDRRYHSLLKQ
CCEECEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA