Click here to switch to the map view.

The map label for this gene is psdht [H]

Identifier: 157369881

GI number: 157369881

Start: 1794609

End: 1795577

Strand: Direct

Name: psdht [H]

Synonym: Spro_1638

Alternate gene names: 157369881

Gene position: 1794609-1795577 (Clockwise)

Preceding gene: 157369880

Following gene: 157369883

Centisome position: 32.94

GC content: 52.53

Gene sequence:

>969_bases
ATGACAAAATTAATTGAATTTACCGCCATTACTGAAGCAGCAGAACGGCTCAATGGGAAAGTTATTCGCACCGCCACTAT
TCCAGCACCGAAACTGACTGAAAAACTACAGAGGCCGGTTTTCCTGAAACTTGAAAATACTCAGTTGGGGGGCTCTTTTA
AAGCCAGGGGCGTGCTTAACAAATTTTTGAGCCTGGCGGACGATGTTAAAGATAAACGGTTTGTGGCGGTGAGCGGCGGT
AATTTTGGCATTGCGATAGCCGAGGCGGCGAAGATCTTTGGGGCTGATGTCACTATTATTATGCCGCAAAGTGCCCCGGC
AACCGCCATCGACCGGGTGGGTGAACTGGGCAGCTCTGTTATCGTGGAATCTGATGTTCACGCTGCGTTTGAGCGGGCCA
AGGCCTTGGCTGACCAGGGATACGTAGTCATTGATGATTGCAACGACTCGCTGATTGCTGAAGGACACGGAACCCTGGCA
CTTGAGTTCATTGCCGATTGCCCGGAACTTACCGACGTCCTGGTTGCGGTGGGCGGCGGCGGTATGCTGGCTGGTGTGGC
TACTGCGCTAAAAGCCATAAGGCCAGAGATGAGAATTTGGGGGGTTGAAACCTCTGGGGCAAGTTCGATGCACCAGGCAT
TGCGCGCCGGCAAACCGGTGAACATTGAGGTGACTTCAATTATCTCTACCCTCGGCGTGCCTGTCATTGATGAAATGATG
CTGGCCCATGCTCAAGCTTATATCGAGGAAGTGGTTGTGGTATCGGATAAGGCAGCGGTAGAGGGAATGGTGGCCTTTGG
CGAAGATGCAAAATTATGGGTTGAACTGGCGTCCGGGACGCTGATACCTGCGGCTATGGTCATTGCCCCCAAGCTTCCCG
CTAACGCCGTCATTGGGATGGTAGTGTGCGGTGGAAATATCTCCCATCAGGACATGTCGAAATGGGTTAGCTACTCGCAA
ACCCTGTGA

Upstream 100 bases:

>100_bases
CAGTAAAAGTCTGTAGTTTGACGTGTATTCCGATAAAGGCTGCAGGATTAAAATGTCAGCTTTATATGCGCAACAATAAA
ATTCACCTGACTGGGCAAAA

Downstream 100 bases:

>100_bases
TTTTTCTCCTGCGGAGTTTATTCAGGCAATAAAAAACCCGCTAGTCTTGAACCTAAATAGGCGGGACTAACGGGCTCCAC
AAAATGGGGACATCAAAGAA

Product: pyridoxal-5'-phosphate-dependent protein subunit beta

Products: @OXOB02.txt*Oxobutanoate!; NH3; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTKLIEFTAITEAAERLNGKVIRTATIPAPKLTEKLQRPVFLKLENTQLGGSFKARGVLNKFLSLADDVKDKRFVAVSGG
NFGIAIAEAAKIFGADVTIIMPQSAPATAIDRVGELGSSVIVESDVHAAFERAKALADQGYVVIDDCNDSLIAEGHGTLA
LEFIADCPELTDVLVAVGGGGMLAGVATALKAIRPEMRIWGVETSGASSMHQALRAGKPVNIEVTSIISTLGVPVIDEMM
LAHAQAYIEEVVVVSDKAAVEGMVAFGEDAKLWVELASGTLIPAAMVIAPKLPANAVIGMVVCGGNISHQDMSKWVSYSQ
TL

Sequences:

>Translated_322_residues
MTKLIEFTAITEAAERLNGKVIRTATIPAPKLTEKLQRPVFLKLENTQLGGSFKARGVLNKFLSLADDVKDKRFVAVSGG
NFGIAIAEAAKIFGADVTIIMPQSAPATAIDRVGELGSSVIVESDVHAAFERAKALADQGYVVIDDCNDSLIAEGHGTLA
LEFIADCPELTDVLVAVGGGGMLAGVATALKAIRPEMRIWGVETSGASSMHQALRAGKPVNIEVTSIISTLGVPVIDEMM
LAHAQAYIEEVVVVSDKAAVEGMVAFGEDAKLWVELASGTLIPAAMVIAPKLPANAVIGMVVCGGNISHQDMSKWVSYSQ
TL
>Mature_321_residues
TKLIEFTAITEAAERLNGKVIRTATIPAPKLTEKLQRPVFLKLENTQLGGSFKARGVLNKFLSLADDVKDKRFVAVSGGN
FGIAIAEAAKIFGADVTIIMPQSAPATAIDRVGELGSSVIVESDVHAAFERAKALADQGYVVIDDCNDSLIAEGHGTLAL
EFIADCPELTDVLVAVGGGGMLAGVATALKAIRPEMRIWGVETSGASSMHQALRAGKPVNIEVTSIISTLGVPVIDEMML
AHAQAYIEEVVVVSDKAAVEGMVAFGEDAKLWVELASGTLIPAAMVIAPKLPANAVIGMVVCGGNISHQDMSKWVSYSQT
L

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=336, Percent_Identity=27.9761904761905, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI33469958, Length=290, Percent_Identity=35.5172413793103, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI19923959, Length=284, Percent_Identity=32.7464788732394, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789505, Length=270, Percent_Identity=28.8888888888889, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790207, Length=295, Percent_Identity=28.135593220339, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71991565, Length=318, Percent_Identity=29.559748427673, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17537387, Length=220, Percent_Identity=32.2727272727273, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17508781, Length=322, Percent_Identity=26.3975155279503, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322631, Length=314, Percent_Identity=27.7070063694268, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319788, Length=293, Percent_Identity=26.2798634812287, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320930, Length=261, Percent_Identity=31.4176245210728, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322024, Length=185, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21355833, Length=311, Percent_Identity=30.8681672025724, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19 [C]

Molecular weight: Translated: 33798; Mature: 33666

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLIEFTAITEAAERLNGKVIRTATIPAPKLTEKLQRPVFLKLENTQLGGSFKARGVLN
CCCEEHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
KFLSLADDVKDKRFVAVSGGNFGIAIAEAAKIFGADVTIIMPQSAPATAIDRVGELGSSV
HHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCE
IVESDVHAAFERAKALADQGYVVIDDCNDSLIAEGHGTLALEFIADCPELTDVLVAVGGG
EEEHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCEEEEEHHCCHHHHHHHEEECCC
GMLAGVATALKAIRPEMRIWGVETSGASSMHQALRAGKPVNIEVTSIISTLGVPVIDEMM
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHHH
LAHAQAYIEEVVVVSDKAAVEGMVAFGEDAKLWVELASGTLIPAAMVIAPKLPANAVIGM
HHHHHHHHHHHHEECCCHHHHHHEEECCCCEEEEEECCCCCHHHHHEEECCCCCCCEEEE
VVCGGNISHQDMSKWVSYSQTL
EEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKLIEFTAITEAAERLNGKVIRTATIPAPKLTEKLQRPVFLKLENTQLGGSFKARGVLN
CCEEHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
KFLSLADDVKDKRFVAVSGGNFGIAIAEAAKIFGADVTIIMPQSAPATAIDRVGELGSSV
HHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCE
IVESDVHAAFERAKALADQGYVVIDDCNDSLIAEGHGTLALEFIADCPELTDVLVAVGGG
EEEHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCEEEEEHHCCHHHHHHHEEECCC
GMLAGVATALKAIRPEMRIWGVETSGASSMHQALRAGKPVNIEVTSIISTLGVPVIDEMM
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHHH
LAHAQAYIEEVVVVSDKAAVEGMVAFGEDAKLWVELASGTLIPAAMVIAPKLPANAVIGM
HHHHHHHHHHHHEECCCHHHHHHEEECCCCEEEEEECCCCCHHHHHEEECCCCCCCEEEE
VVCGGNISHQDMSKWVSYSQTL
EEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: @THRE01.txt*L-threonine!; H2O [C]

Specific reaction: @THRE01.txt*L-threonine! + H2O = @OXOB02.txt*oxobutanoate! + NH3 + H2O [C]

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]