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The map label for this gene is galS [H]

Identifier: 157369805

GI number: 157369805

Start: 1701877

End: 1702914

Strand: Direct

Name: galS [H]

Synonym: Spro_1562

Alternate gene names: 157369805

Gene position: 1701877-1702914 (Clockwise)

Preceding gene: 157369804

Following gene: 157369806

Centisome position: 31.23

GC content: 59.25

Gene sequence:

>1038_bases
ATGACCTCTGTTCACCCAACCACCATTCGCGACGTGGCGAAGCGTGCGGGCGTGTCCGTGGCGACCGTTTCCCGCGTGCT
GAACCACAGCGCCCTGACCAGTAAAGAGACGCGTGAACAGGTGCTTAAGGCGGTGGCTGAACTGGGTTATCGGCCCAACG
CCAATGCACAGGCGTTGGCCACCCAGAGCAGCGATACCCTCGGGGTGGTGGTGATGGACGTTTCCGATCCCTTCTTTGGT
GCGCTGGTGAAAGCGGTAGACACCGTAGCGCAGCAACACCACAAATATTTGCTGATCGGCAACAGTTATCATCAGGCAGA
CAAAGAGCGTCACGCTATCGAAGTGCTGATTCGCCAGCGCTGTAATGCCTTGATTGTGCATGCTAAAGCGCTGTGCGATG
CCGAATTGATGACTTTCCTCGACCAGGTGCCGGGCATGGTGTTGATCAACCGTATCATTGCCGGCTATGAGCATCGCTGC
GTTGGCCTGGACAACGTCAGCGGCGCGGAGATGGCAATGCGTTTGCTGCTGTCGCAGGGGCATCAGCGCATTGGCTATCT
GGGCTCCAACCACCCGATAGAAGACGGGCCGCTGCGCCAGCAGGGCTATACGCAGGCGATGGCTGCGGCTGGGTTGGTTA
CGCCGGACAATTGGCGTGCCTATGGTTCGCCGGATTTGCAGGGTGGGGAAGCGGCAATGGTCGAGTTACTCGGGCGTAAC
CTGCATTTGAGCGCGGTGTTTGCTTACAACGATGCCATGGCTGCCGGTGCCATGGCGGTGCTGAAAGAAAACGCTATCAC
AGTACCGCAAAATTTCTCGCTGGTGGGGTTCGATGATATCCCCATCGCACGCTATACCAGCCCCAAGCTCACCACGGTGC
GCTATCCCATTGTCACCATGGCGACGCTGGCCACCGAGTTGGCCCTGCTGGGCGCGGCAGGCCGGCTGGAGCCGCAGGAC
CGCCATTTGTTTATGCCCACTCTGGTACGCCGCCATTCTGTGGCTCCGTGGCAAATTGAGGCAGCGGTCACTCTCTGA

Upstream 100 bases:

>100_bases
ATGTAAACGTTTTCTTTCCTGTGACGGGATTCACGCAGAGGAGGATGACAAACGGGGTAGGATAGCGCCACTGTCAACTA
CCCCGACCTCAGGATTTTTC

Downstream 100 bases:

>100_bases
ACATTTAATCTTGTGTAACCGTTTTCAATCTGTGAGAAAATTCACAGATTATTAACATCACGCCGTCTATGCTCTAGTCG
TTTTCCAGCGTAAAGGCACT

Product: DNA-binding transcriptional regulator GalS

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTSVHPTTIRDVAKRAGVSVATVSRVLNHSALTSKETREQVLKAVAELGYRPNANAQALATQSSDTLGVVVMDVSDPFFG
ALVKAVDTVAQQHHKYLLIGNSYHQADKERHAIEVLIRQRCNALIVHAKALCDAELMTFLDQVPGMVLINRIIAGYEHRC
VGLDNVSGAEMAMRLLLSQGHQRIGYLGSNHPIEDGPLRQQGYTQAMAAAGLVTPDNWRAYGSPDLQGGEAAMVELLGRN
LHLSAVFAYNDAMAAGAMAVLKENAITVPQNFSLVGFDDIPIARYTSPKLTTVRYPIVTMATLATELALLGAAGRLEPQD
RHLFMPTLVRRHSVAPWQIEAAVTL

Sequences:

>Translated_345_residues
MTSVHPTTIRDVAKRAGVSVATVSRVLNHSALTSKETREQVLKAVAELGYRPNANAQALATQSSDTLGVVVMDVSDPFFG
ALVKAVDTVAQQHHKYLLIGNSYHQADKERHAIEVLIRQRCNALIVHAKALCDAELMTFLDQVPGMVLINRIIAGYEHRC
VGLDNVSGAEMAMRLLLSQGHQRIGYLGSNHPIEDGPLRQQGYTQAMAAAGLVTPDNWRAYGSPDLQGGEAAMVELLGRN
LHLSAVFAYNDAMAAGAMAVLKENAITVPQNFSLVGFDDIPIARYTSPKLTTVRYPIVTMATLATELALLGAAGRLEPQD
RHLFMPTLVRRHSVAPWQIEAAVTL
>Mature_344_residues
TSVHPTTIRDVAKRAGVSVATVSRVLNHSALTSKETREQVLKAVAELGYRPNANAQALATQSSDTLGVVVMDVSDPFFGA
LVKAVDTVAQQHHKYLLIGNSYHQADKERHAIEVLIRQRCNALIVHAKALCDAELMTFLDQVPGMVLINRIIAGYEHRCV
GLDNVSGAEMAMRLLLSQGHQRIGYLGSNHPIEDGPLRQQGYTQAMAAAGLVTPDNWRAYGSPDLQGGEAAMVELLGRNL
HLSAVFAYNDAMAAGAMAVLKENAITVPQNFSLVGFDDIPIARYTSPKLTTVRYPIVTMATLATELALLGAAGRLEPQDR
HLFMPTLVRRHSVAPWQIEAAVTL

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence (corresponding to residues 15 to 20) [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=70.919881305638, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI1789202, Length=335, Percent_Identity=57.6119402985075, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=36.0119047619048, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1790369, Length=289, Percent_Identity=32.8719723183391, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=31.8318318318318, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1790194, Length=334, Percent_Identity=30.5389221556886, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=27.7258566978193, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=29.5819935691318, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI48994940, Length=305, Percent_Identity=26.2295081967213, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1786540, Length=350, Percent_Identity=28.2857142857143, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787906, Length=307, Percent_Identity=27.3615635179153, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789456, Length=305, Percent_Identity=28.5245901639344, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790689, Length=314, Percent_Identity=24.203821656051, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786268, Length=280, Percent_Identity=26.7857142857143, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37405; Mature: 37273

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVHPTTIRDVAKRAGVSVATVSRVLNHSALTSKETREQVLKAVAELGYRPNANAQALA
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEE
TQSSDTLGVVVMDVSDPFFGALVKAVDTVAQQHHKYLLIGNSYHQADKERHAIEVLIRQR
ECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHH
CNALIVHAKALCDAELMTFLDQVPGMVLINRIIAGYEHRCVGLDNVSGAEMAMRLLLSQG
CCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCC
HQRIGYLGSNHPIEDGPLRQQGYTQAMAAAGLVTPDNWRAYGSPDLQGGEAAMVELLGRN
CCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
LHLSAVFAYNDAMAAGAMAVLKENAITVPQNFSLVGFDDIPIARYTSPKLTTVRYPIVTM
CEEEEEEEECHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCCEECCCCCEEEECHHHHH
ATLATELALLGAAGRLEPQDRHLFMPTLVRRHSVAPWQIEAAVTL
HHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHCCCCCEEEEEEEEC
>Mature Secondary Structure 
TSVHPTTIRDVAKRAGVSVATVSRVLNHSALTSKETREQVLKAVAELGYRPNANAQALA
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEE
TQSSDTLGVVVMDVSDPFFGALVKAVDTVAQQHHKYLLIGNSYHQADKERHAIEVLIRQR
ECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHH
CNALIVHAKALCDAELMTFLDQVPGMVLINRIIAGYEHRCVGLDNVSGAEMAMRLLLSQG
CCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCC
HQRIGYLGSNHPIEDGPLRQQGYTQAMAAAGLVTPDNWRAYGSPDLQGGEAAMVELLGRN
CCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
LHLSAVFAYNDAMAAGAMAVLKENAITVPQNFSLVGFDDIPIARYTSPKLTTVRYPIVTM
CEEEEEEEECHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCCEECCCCCEEEECHHHHH
ATLATELALLGAAGRLEPQDRHLFMPTLVRRHSVAPWQIEAAVTL
HHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1619663; 9278503; 8208287; 8982002 [H]