Click here to switch to the map view.

The map label for this gene is argT [H]

Identifier: 157369611

GI number: 157369611

Start: 1497105

End: 1497884

Strand: Reverse

Name: argT [H]

Synonym: Spro_1368

Alternate gene names: 157369611

Gene position: 1497884-1497105 (Counterclockwise)

Preceding gene: 157369612

Following gene: 157369609

Centisome position: 27.49

GC content: 59.36

Gene sequence:

>780_bases
ATGAAATCACTGAAAACGTTGATCGCCGCAGGCTGCCTGCTGGCCGCCGGTTCCACGCTGGCGGCGGAAAACACCCTGCG
CTTCGGGCTGGAAGCGCTGTACCCACCGTTCGAATCCAAATCGGCCAGCGGCAAGCTGGAAGGTTTTGATATCGATCTGG
GCAACGCGGTGTGTGCCGCCGCCCAGTTAAAATGCAGTTGGGTCGAGACCTCGTTCGACAGCCTGATCCCGGCGTTGAAG
GCGCGCAAGTTCGACGCCATCAATTCGGCGATGAACGTCACCGACCAGCGTCGTCAGGCAATAGACTTTACCGATTCAAT
CTATCAGGTGCCGAATCGCCTGATCGCCAAGGCCGACAGCGGTCTGCTGCCAAACGCCACCTCGCTGGCGGGCAAGCATG
TCGGCGTGCTGCAAGGCTCGATTCAGGAAACCTACGCCAAGGCCCACTGGGCGCCGCAGGGTGTGGACGTGGTGTCCTAT
CAGGATCAGAACCAGGCTTATCTTGACCTGACCGCCGGGCGGCTCGACGCAACGCTGGTCATGGCACCGTCAGGCCAAAG
CGGTTTCCTGGCGCACCCGGACGGTAAGGGTTTCGCCTTTGTCGGCGATGCGGTCAGCGACGACAAAATCCTCGGTGAGG
GCATTGCCTTCGGCCTGCGTAAGGGTGATGACGCGCTGAAGAAAAAGCTGAATGAGGCGATCGTCAAAGTGAAGCAGCAA
GGCACCGTGAGCGAGCTGGCGAAGAAGTATTTTGGCGATATTGACGTGACGGTGAAGTAA

Upstream 100 bases:

>100_bases
TTTTGCAAAAGACAATGAGACCCTGCTGGAGGGCGCACGCCGTCTGTGTCAGGTATAAGAAATTTATTTTTGCTTTGACC
ATGGGAAAAGGGCTCCAACT

Downstream 100 bases:

>100_bases
GAAACCAGCTTCAATCGCCATTGACTATAAAACAGACTGAGCGGTTTGTAATCAAACAAAACACCGGGTACACTGAAAAA
AGGATGACAAGTATGTGCGA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKSLKTLIAAGCLLAAGSTLAAENTLRFGLEALYPPFESKSASGKLEGFDIDLGNAVCAAAQLKCSWVETSFDSLIPALK
ARKFDAINSAMNVTDQRRQAIDFTDSIYQVPNRLIAKADSGLLPNATSLAGKHVGVLQGSIQETYAKAHWAPQGVDVVSY
QDQNQAYLDLTAGRLDATLVMAPSGQSGFLAHPDGKGFAFVGDAVSDDKILGEGIAFGLRKGDDALKKKLNEAIVKVKQQ
GTVSELAKKYFGDIDVTVK

Sequences:

>Translated_259_residues
MKSLKTLIAAGCLLAAGSTLAAENTLRFGLEALYPPFESKSASGKLEGFDIDLGNAVCAAAQLKCSWVETSFDSLIPALK
ARKFDAINSAMNVTDQRRQAIDFTDSIYQVPNRLIAKADSGLLPNATSLAGKHVGVLQGSIQETYAKAHWAPQGVDVVSY
QDQNQAYLDLTAGRLDATLVMAPSGQSGFLAHPDGKGFAFVGDAVSDDKILGEGIAFGLRKGDDALKKKLNEAIVKVKQQ
GTVSELAKKYFGDIDVTVK
>Mature_259_residues
MKSLKTLIAAGCLLAAGSTLAAENTLRFGLEALYPPFESKSASGKLEGFDIDLGNAVCAAAQLKCSWVETSFDSLIPALK
ARKFDAINSAMNVTDQRRQAIDFTDSIYQVPNRLIAKADSGLLPNATSLAGKHVGVLQGSIQETYAKAHWAPQGVDVVSY
QDQNQAYLDLTAGRLDATLVMAPSGQSGFLAHPDGKGFAFVGDAVSDDKILGEGIAFGLRKGDDALKKKLNEAIVKVKQQ
GTVSELAKKYFGDIDVTVK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=241, Percent_Identity=50.207468879668, Blast_Score=241, Evalue=3e-65,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=46.9827586206897, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1787085, Length=248, Percent_Identity=32.6612903225806, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=35.4581673306773, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788228, Length=257, Percent_Identity=31.5175097276265, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787031, Length=254, Percent_Identity=33.4645669291339, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27500; Mature: 27500

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLKTLIAAGCLLAAGSTLAAENTLRFGLEALYPPFESKSASGKLEGFDIDLGNAVCAA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHH
AQLKCSWVETSFDSLIPALKARKFDAINSAMNVTDQRRQAIDFTDSIYQVPNRLIAKADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHCCC
GLLPNATSLAGKHVGVLQGSIQETYAKAHWAPQGVDVVSYQDQNQAYLDLTAGRLDATLV
CCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCEEEE
MAPSGQSGFLAHPDGKGFAFVGDAVSDDKILGEGIAFGLRKGDDALKKKLNEAIVKVKQQ
ECCCCCCCCEECCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GTVSELAKKYFGDIDVTVK
CCHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKSLKTLIAAGCLLAAGSTLAAENTLRFGLEALYPPFESKSASGKLEGFDIDLGNAVCAA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHH
AQLKCSWVETSFDSLIPALKARKFDAINSAMNVTDQRRQAIDFTDSIYQVPNRLIAKADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHCCC
GLLPNATSLAGKHVGVLQGSIQETYAKAHWAPQGVDVVSYQDQNQAYLDLTAGRLDATLV
CCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCEEEE
MAPSGQSGFLAHPDGKGFAFVGDAVSDDKILGEGIAFGLRKGDDALKKKLNEAIVKVKQQ
ECCCCCCCCEECCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GTVSELAKKYFGDIDVTVK
CCHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]