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The map label for this gene is gcvA [H]

Identifier: 157369582

GI number: 157369582

Start: 1469678

End: 1470616

Strand: Reverse

Name: gcvA [H]

Synonym: Spro_1339

Alternate gene names: 157369582

Gene position: 1470616-1469678 (Counterclockwise)

Preceding gene: 157369584

Following gene: 157369581

Centisome position: 26.99

GC content: 63.26

Gene sequence:

>939_bases
ATGAAACTCAAGCTGAACGCTCAACCTCGCCTGCCCTCCCTGAAAGCCTTGCACACCTTCGAGGTGGCGGCACGCTGCGC
CAGTTTTACCGAGGCAGCGGAGGAGTTGAACGTCACGCTGGGGGCGGTCAGCCGCCAGATACGTCACCTGGAAAATGAAC
TGGGCGTCGAGCTGTTTCACCGCAAGGGGAACGCGGTGGCGCTAAACGACACCGGCAAACAACTGGCCATCGACGTGACT
CGCGCACTGACGTTGCTGCAAAAAGCCAGCGATCGCGTCCGGCCAGAGCAACAGCAAACGCTGAAGCTGACCTGCACGCT
GGGCATTGCCAGCCATTGGCTGGCGGCACGACTACCGGCACTGCGCCGTGCCGATCCTGATGTTTCACTGGTGCTGGACG
CCAACGAAAGCCTGCGTGACCTGGCCAATGGCGAAGCAGACATCGCCATCCGTTACAGCCCCACCGCCAATCCGCCGCCA
CACTCCCGCGAGCTGCTAGCCGATACCTTCGCGCCGCTGGCAAGCCCGGCCTTTCTTGCCGCGCACCCTCGTCGCATTAC
GGCCACCGATTTGGCGCTGTCGCAATTGATCCACTCGCCGTGGAAGGATGGACAGAAGATGGGCATAGCCGGTTGGCAAG
ACTGGTTCGCCGCCTTTGGCGAAGGCACCCCGGCACCGCGCGGTATTCTCACCTTTAACAGCGTCGGCCATGCCCTGCAG
GAAGCGATAGAAGGTAACGGCATCGTACTCGGCAGCCTGGCAGTCGCGCAGGATGCCCTGGCCACCGGTAAGCTGGTTCG
GGTCTTTGGCGACGACCGACAACTGCCCGCCGCCTACCACTACCGCCTGTGCTGGGCGGACAGCGCGTTCGTTAGCGCCA
CCACCCGCCGCTGGGTGAATCTGCTGCTGCAGGCGGCAGACCAACCCCCAGCGTTCTGA

Upstream 100 bases:

>100_bases
GCCCCATCACAATGTCCACACGATACTCCCGTCGAATATTTGCCCTAAAAGTAAGCAAAAAATCACTTGTGGAACATCGA
TAGATTGGCAATATGGTTGA

Downstream 100 bases:

>100_bases
CGCAGCACGTATTGCGCCCACAGCAGTGAAAAATCCAACTATTTTATAATCAATTTTTTTGCTGCGAGACACACCGTGGA
CAGACAACGCTACCTGCAAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKLKLNAQPRLPSLKALHTFEVAARCASFTEAAEELNVTLGAVSRQIRHLENELGVELFHRKGNAVALNDTGKQLAIDVT
RALTLLQKASDRVRPEQQQTLKLTCTLGIASHWLAARLPALRRADPDVSLVLDANESLRDLANGEADIAIRYSPTANPPP
HSRELLADTFAPLASPAFLAAHPRRITATDLALSQLIHSPWKDGQKMGIAGWQDWFAAFGEGTPAPRGILTFNSVGHALQ
EAIEGNGIVLGSLAVAQDALATGKLVRVFGDDRQLPAAYHYRLCWADSAFVSATTRRWVNLLLQAADQPPAF

Sequences:

>Translated_312_residues
MKLKLNAQPRLPSLKALHTFEVAARCASFTEAAEELNVTLGAVSRQIRHLENELGVELFHRKGNAVALNDTGKQLAIDVT
RALTLLQKASDRVRPEQQQTLKLTCTLGIASHWLAARLPALRRADPDVSLVLDANESLRDLANGEADIAIRYSPTANPPP
HSRELLADTFAPLASPAFLAAHPRRITATDLALSQLIHSPWKDGQKMGIAGWQDWFAAFGEGTPAPRGILTFNSVGHALQ
EAIEGNGIVLGSLAVAQDALATGKLVRVFGDDRQLPAAYHYRLCWADSAFVSATTRRWVNLLLQAADQPPAF
>Mature_312_residues
MKLKLNAQPRLPSLKALHTFEVAARCASFTEAAEELNVTLGAVSRQIRHLENELGVELFHRKGNAVALNDTGKQLAIDVT
RALTLLQKASDRVRPEQQQTLKLTCTLGIASHWLAARLPALRRADPDVSLVLDANESLRDLANGEADIAIRYSPTANPPP
HSRELLADTFAPLASPAFLAAHPRRITATDLALSQLIHSPWKDGQKMGIAGWQDWFAAFGEGTPAPRGILTFNSVGHALQ
EAIEGNGIVLGSLAVAQDALATGKLVRVFGDDRQLPAAYHYRLCWADSAFVSATTRRWVNLLLQAADQPPAF

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=306, Percent_Identity=30.718954248366, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788706, Length=262, Percent_Identity=33.969465648855, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786448, Length=253, Percent_Identity=30.4347826086957, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081978, Length=312, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=25, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789440, Length=266, Percent_Identity=27.4436090225564, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=262, Percent_Identity=25.9541984732824, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=163, Percent_Identity=30.0613496932515, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788887, Length=213, Percent_Identity=29.1079812206573, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33877; Mature: 33877

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKLNAQPRLPSLKALHTFEVAARCASFTEAAEELNVTLGAVSRQIRHLENELGVELFH
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEE
RKGNAVALNDTGKQLAIDVTRALTLLQKASDRVRPEQQQTLKLTCTLGIASHWLAARLPA
CCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHHHHHHCCH
LRRADPDVSLVLDANESLRDLANGEADIAIRYSPTANPPPHSRELLADTFAPLASPAFLA
HHCCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHH
AHPRRITATDLALSQLIHSPWKDGQKMGIAGWQDWFAAFGEGTPAPRGILTFNSVGHALQ
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEHHHHHHHH
EAIEGNGIVLGSLAVAQDALATGKLVRVFGDDRQLPAAYHYRLCWADSAFVSATTRRWVN
HHHCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCCHHHEEEEEECCCHHHHHHHHHHHH
LLLQAADQPPAF
HHHHHCCCCCCC
>Mature Secondary Structure
MKLKLNAQPRLPSLKALHTFEVAARCASFTEAAEELNVTLGAVSRQIRHLENELGVELFH
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEE
RKGNAVALNDTGKQLAIDVTRALTLLQKASDRVRPEQQQTLKLTCTLGIASHWLAARLPA
CCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHHHHHHCCH
LRRADPDVSLVLDANESLRDLANGEADIAIRYSPTANPPPHSRELLADTFAPLASPAFLA
HHCCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHH
AHPRRITATDLALSQLIHSPWKDGQKMGIAGWQDWFAAFGEGTPAPRGILTFNSVGHALQ
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEHHHHHHHH
EAIEGNGIVLGSLAVAQDALATGKLVRVFGDDRQLPAAYHYRLCWADSAFVSATTRRWVN
HHHCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCCHHHEEEEEECCCHHHHHHHHHHHH
LLLQAADQPPAF
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]