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The map label for this gene is tolB

Identifier: 157369522

GI number: 157369522

Start: 1405700

End: 1406992

Strand: Direct

Name: tolB

Synonym: Spro_1279

Alternate gene names: 157369522

Gene position: 1405700-1406992 (Clockwise)

Preceding gene: 157369521

Following gene: 157369523

Centisome position: 25.8

GC content: 56.61

Gene sequence:

>1293_bases
ATGAAGCAGGCATTTCGAGTAGCGCTAGGTTTTTTAATACTGTGGGCATCCGTTCTTCACGCGGAAGTTCGCATTGAAAT
CACCCAAGGGGTCGACTCCGCTCGTCCGATTGGCGTGGTGCCGTTTAAATGGGCGGGCCCAGGCACGCCTCCGGAAGATA
TTGGCAAAATTGTCGGCGCAGACCTGCGCAACAGCGGCAAATTCAACCCAATTGACGTGGCGCGTATGCCTCAGCAACCT
ACCACCGCGTCTGAAGTGACGCCGGCGGCCTGGACTGCATTGGGCATTGACGCGGTCGTGGTCGGCCAGGTGCAACCTGG
TGCAGACGGCGGCTACCTGATCTCTTATCAACTGGTGGACACTTCGGGCTCACCAGGCACCGTTCTGGCGCAGAACCAGT
ATAAAGTGACCAAACAATGGTTGCGTTATTCTGCTCACACCGCCAGTGACGAAGTGTTTGAGAAGCTGACCGGTATCAAG
GGCGCATTCCGTACCCGTATCGCATACGTAGTGCAGACCAACGGCGGGAAATTCCCGTATGAGCTGCGCGTAGCGGATTA
CGACGGCTACAACCAGTTTACGGTACATCGTTCACCAGAACCTCTGATGTCCCCGGCCTGGTCGCCGGACGGCAGCAAAC
TGGCGTACGTAACCTTTGAAAGCGGCCGTTCTGCGCTGGTTGTGCAGACCCTGGCTAACGGTGCTATCCGTCAGATCGCT
TCGTTCCCGCGTCACAACGGGGCGCCGGCGTTCTCACCGGACGGCAGCCGCCTGGCGTTTGCGCTGTCTAAAAGCGGTAG
CCTGAACCTGTACGTGATGAATCTTGGCTCTGGCCAGATCACCCAAATCACCGACGGGCGCAGCAACAATACCGAACCGA
CCTGGTTCCCGGATGGGCAGTCTCTGGCCTATACCTCCGATCAGGGCGGCAGACCACAGATTTATAAAGTCAGTGCCAGT
GGCGGTGCGTCACAGCGTCTGACATGGGAAGGCTCTCAGAATCAGGATTCTGAAGTCAGCTCTGATGGTAAATTCCTGGT
GATGGTGAGCTCTAACAGTGGTGCTCAGCATATCGCCAAACAAGATCTCGGATCGGGGGCGGTTCAAGTTTTGACCGGCA
CCTTCCTGGACGAAACGCCTAGTATTGCGCCAAATGGCACCATGGTGATCTATAGCTCCACGCAAGGTATGGGCTCCGTG
CTGCAACTGGTATCGACTGATGGGCGTTTTAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTC
GCCGTATCTGTGA

Upstream 100 bases:

>100_bases
TACTAAGGTAACCAACAGGGATTATGTAGTTTTGTTTGAGTTGGTTTGTTAAAATTCTGCTAATTTATCGCAGGCATTCC
GCCTGGATAAGGGAGATGAG

Downstream 100 bases:

>100_bases
TGCATGTGTAAATATGTATGGCAAACTATAAAAGGATTAAAGAAATGCAACTGAACAAAGTGCTGAAAGGGCTTCTGCTG
GCACTGCCAGTTCTGGCTGT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKQAFRVALGFLILWASVLHAEVRIEITQGVDSARPIGVVPFKWAGPGTPPEDIGKIVGADLRNSGKFNPIDVARMPQQP
TTASEVTPAAWTALGIDAVVVGQVQPGADGGYLISYQLVDTSGSPGTVLAQNQYKVTKQWLRYSAHTASDEVFEKLTGIK
GAFRTRIAYVVQTNGGKFPYELRVADYDGYNQFTVHRSPEPLMSPAWSPDGSKLAYVTFESGRSALVVQTLANGAIRQIA
SFPRHNGAPAFSPDGSRLAFALSKSGSLNLYVMNLGSGQITQITDGRSNNTEPTWFPDGQSLAYTSDQGGRPQIYKVSAS
GGASQRLTWEGSQNQDSEVSSDGKFLVMVSSNSGAQHIAKQDLGSGAVQVLTGTFLDETPSIAPNGTMVIYSSTQGMGSV
LQLVSTDGRFKARLPATDGQVKFPAWSPYL

Sequences:

>Translated_430_residues
MKQAFRVALGFLILWASVLHAEVRIEITQGVDSARPIGVVPFKWAGPGTPPEDIGKIVGADLRNSGKFNPIDVARMPQQP
TTASEVTPAAWTALGIDAVVVGQVQPGADGGYLISYQLVDTSGSPGTVLAQNQYKVTKQWLRYSAHTASDEVFEKLTGIK
GAFRTRIAYVVQTNGGKFPYELRVADYDGYNQFTVHRSPEPLMSPAWSPDGSKLAYVTFESGRSALVVQTLANGAIRQIA
SFPRHNGAPAFSPDGSRLAFALSKSGSLNLYVMNLGSGQITQITDGRSNNTEPTWFPDGQSLAYTSDQGGRPQIYKVSAS
GGASQRLTWEGSQNQDSEVSSDGKFLVMVSSNSGAQHIAKQDLGSGAVQVLTGTFLDETPSIAPNGTMVIYSSTQGMGSV
LQLVSTDGRFKARLPATDGQVKFPAWSPYL
>Mature_430_residues
MKQAFRVALGFLILWASVLHAEVRIEITQGVDSARPIGVVPFKWAGPGTPPEDIGKIVGADLRNSGKFNPIDVARMPQQP
TTASEVTPAAWTALGIDAVVVGQVQPGADGGYLISYQLVDTSGSPGTVLAQNQYKVTKQWLRYSAHTASDEVFEKLTGIK
GAFRTRIAYVVQTNGGKFPYELRVADYDGYNQFTVHRSPEPLMSPAWSPDGSKLAYVTFESGRSALVVQTLANGAIRQIA
SFPRHNGAPAFSPDGSRLAFALSKSGSLNLYVMNLGSGQITQITDGRSNNTEPTWFPDGQSLAYTSDQGGRPQIYKVSAS
GGASQRLTWEGSQNQDSEVSSDGKFLVMVSSNSGAQHIAKQDLGSGAVQVLTGTFLDETPSIAPNGTMVIYSSTQGMGSV
LQLVSTDGRFKARLPATDGQVKFPAWSPYL

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=430, Percent_Identity=84.1860465116279, Blast_Score=760, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_SERP5 (A8GB93)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477511.1
- ProteinModelPortal:   A8GB93
- SMR:   A8GB93
- STRING:   A8GB93
- GeneID:   5604787
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1279
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK03629
- BioCyc:   SPRO399741:SPRO_1279-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 45947; Mature: 45947

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAFRVALGFLILWASVLHAEVRIEITQGVDSARPIGVVPFKWAGPGTPPEDIGKIVGA
CCHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCC
DLRNSGKFNPIDVARMPQQPTTASEVTPAAWTALGIDAVVVGQVQPGADGGYLISYQLVD
CCCCCCCCCCEEHHCCCCCCCCHHCCCCHHHHHCCCCEEEEEEECCCCCCCEEEEEEEEE
TSGSPGTVLAQNQYKVTKQWLRYSAHTASDEVFEKLTGIKGAFRTRIAYVVQTNGGKFPY
CCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHEEEEEEEEECCCCCCCE
ELRVADYDGYNQFTVHRSPEPLMSPAWSPDGSKLAYVTFESGRSALVVQTLANGAIRQIA
EEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHH
SFPRHNGAPAFSPDGSRLAFALSKSGSLNLYVMNLGSGQITQITDGRSNNTEPTWFPDGQ
HCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
SLAYTSDQGGRPQIYKVSASGGASQRLTWEGSQNQDSEVSSDGKFLVMVSSNSGAQHIAK
EEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
QDLGSGAVQVLTGTFLDETPSIAPNGTMVIYSSTQGMGSVLQLVSTDGRFKARLPATDGQ
HHCCCCEEEEEEECHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCC
VKFPAWSPYL
EECCCCCCCC
>Mature Secondary Structure
MKQAFRVALGFLILWASVLHAEVRIEITQGVDSARPIGVVPFKWAGPGTPPEDIGKIVGA
CCHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCC
DLRNSGKFNPIDVARMPQQPTTASEVTPAAWTALGIDAVVVGQVQPGADGGYLISYQLVD
CCCCCCCCCCEEHHCCCCCCCCHHCCCCHHHHHCCCCEEEEEEECCCCCCCEEEEEEEEE
TSGSPGTVLAQNQYKVTKQWLRYSAHTASDEVFEKLTGIKGAFRTRIAYVVQTNGGKFPY
CCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHEEEEEEEEECCCCCCCE
ELRVADYDGYNQFTVHRSPEPLMSPAWSPDGSKLAYVTFESGRSALVVQTLANGAIRQIA
EEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHH
SFPRHNGAPAFSPDGSRLAFALSKSGSLNLYVMNLGSGQITQITDGRSNNTEPTWFPDGQ
HCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
SLAYTSDQGGRPQIYKVSASGGASQRLTWEGSQNQDSEVSSDGKFLVMVSSNSGAQHIAK
EEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
QDLGSGAVQVLTGTFLDETPSIAPNGTMVIYSSTQGMGSVLQLVSTDGRFKARLPATDGQ
HHCCCCEEEEEEECHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCC
VKFPAWSPYL
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA