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The map label for this gene is tolA [H]

Identifier: 157369521

GI number: 157369521

Start: 1404329

End: 1405567

Strand: Direct

Name: tolA [H]

Synonym: Spro_1278

Alternate gene names: 157369521

Gene position: 1404329-1405567 (Clockwise)

Preceding gene: 157369520

Following gene: 157369522

Centisome position: 25.77

GC content: 56.26

Gene sequence:

>1239_bases
TTGGTAAAGGCAACTGAGCAAAACGATAAGCTGAACCGCGCCGTTATTGTTTCGGTCGTTCTGCACTTTGTATTGATTGC
CCTGCTGATTTGGGGATCGCTGCAAGAAAACGTCGACATGAACAGTGCCGGCGGTGGCGGCGAAGGTTCGGTCGTCGGTG
CAGTGATGGTCGATCCCGGGGCAGTGGTGGAGCAGTACAACCGCCAGCAGCAGCAGAGTAACGACGCGCAGCGTGCTGAG
AAACAGCGCCAGAAAAAAGCGGAACAGCAGGCCGAAGAACTTCAGCAGAAGCAGGCGGCGGAACAACAGCGCCTGAAAGA
GCTGGAGAAAGAGCGTTTGACCGCGCAGGAAAAAGCGGCTCAGGACGCGAAAGAACAGGCTGAACAGCAAAAGCAGGCCG
CAGAGCAACAAAAGCAGGCAGCTGAACAGCAAAAAGTGGCCGAGAACGCCGCGGCGAAAGCCAAAGAACAACAGAAAGTA
GCGGAAGCCGCTGCCGCCAAGGCCAAGGCCGAAGCGGATAAAGTCGCTAAAGCACAGGCCGACGCGCAGAAGAAAGCCGA
GTCTGAAGCCAAGAAAGAAGCTGCCGCCGCGGCTACCGCCAAGAAACAGGCGGAAGAAGCGAAGAAAGCCGCTGCCGCTG
AAGCTGCAGAGAAAAAAGCCGCTGCCGACGCCGAGAAGAAGGCTGCCGCAGAAGCTGAAAAGGCTGAGAAAAAAGCGGCT
GCTGATGCCGAGAAGAAAGCGGCCGCAGAGGCGGAGAAAAAAGCCGCTGCTGCTGAGAAGGCCGCAGCCAAGAAAGCCGC
AGATGCCAAGAAGAAAGCGGCTGCAGCCGCAGCTGAGCAGTCTTCGGAAGTTGATGATTTGTTTGGTGGCCTGGCGGACG
GTAAAAACGCGCCGAAAGGCGGCGGTGCCAAAGCGAAGGGTGACGGTAAACCGGCCGGTCAGGGCAATGCCAAGACTGCG
GGTGACAAGGGGGTTGATAGCAGTGGTTACGAGGGACAGATTCGCACAGCGATTCAGAACAAGTTCTATGACGCCAGCTC
ATTCGCCGGCAAGACTTGCGATCTACGTATAAAGCTGGGGCCTGATGGCTTTTTGATTGACGTGAAAGCGGTGGGTGGTG
ATCCTGCTCTGTGCCAGGCGGCGGTAGCCGCAGCGAATCAGGCGAGGATTCCTAAACCACCAAGTGATGCCGTTTATCAG
AAAGTTAAGAATTTTACCCTTGTATTCAAACCGCAATAA

Upstream 100 bases:

>100_bases
GATGACCCAGCCAATTTGACCCTAAGGTCAGTGGCAACCCGGTTCCTGCGCTGCGGTGCAGGAACCCCGTATAAGCAACA
CCAGTTTTTGGGAATCTATT

Downstream 100 bases:

>100_bases
CGGTTGATTAGCTAAGTCATATCGCAGGGATGTACTAAGGTAACCAACAGGGATTATGTAGTTTTGTTTGAGTTGGTTTG
TTAAAATTCTGCTAATTTAT

Product: cell envelope integrity inner membrane protein TolA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MVKATEQNDKLNRAVIVSVVLHFVLIALLIWGSLQENVDMNSAGGGGEGSVVGAVMVDPGAVVEQYNRQQQQSNDAQRAE
KQRQKKAEQQAEELQQKQAAEQQRLKELEKERLTAQEKAAQDAKEQAEQQKQAAEQQKQAAEQQKVAENAAAKAKEQQKV
AEAAAAKAKAEADKVAKAQADAQKKAESEAKKEAAAAATAKKQAEEAKKAAAAEAAEKKAAADAEKKAAAEAEKAEKKAA
ADAEKKAAAEAEKKAAAAEKAAAKKAADAKKKAAAAAAEQSSEVDDLFGGLADGKNAPKGGGAKAKGDGKPAGQGNAKTA
GDKGVDSSGYEGQIRTAIQNKFYDASSFAGKTCDLRIKLGPDGFLIDVKAVGGDPALCQAAVAAANQARIPKPPSDAVYQ
KVKNFTLVFKPQ

Sequences:

>Translated_412_residues
MVKATEQNDKLNRAVIVSVVLHFVLIALLIWGSLQENVDMNSAGGGGEGSVVGAVMVDPGAVVEQYNRQQQQSNDAQRAE
KQRQKKAEQQAEELQQKQAAEQQRLKELEKERLTAQEKAAQDAKEQAEQQKQAAEQQKQAAEQQKVAENAAAKAKEQQKV
AEAAAAKAKAEADKVAKAQADAQKKAESEAKKEAAAAATAKKQAEEAKKAAAAEAAEKKAAADAEKKAAAEAEKAEKKAA
ADAEKKAAAEAEKKAAAAEKAAAKKAADAKKKAAAAAAEQSSEVDDLFGGLADGKNAPKGGGAKAKGDGKPAGQGNAKTA
GDKGVDSSGYEGQIRTAIQNKFYDASSFAGKTCDLRIKLGPDGFLIDVKAVGGDPALCQAAVAAANQARIPKPPSDAVYQ
KVKNFTLVFKPQ
>Mature_412_residues
MVKATEQNDKLNRAVIVSVVLHFVLIALLIWGSLQENVDMNSAGGGGEGSVVGAVMVDPGAVVEQYNRQQQQSNDAQRAE
KQRQKKAEQQAEELQQKQAAEQQRLKELEKERLTAQEKAAQDAKEQAEQQKQAAEQQKQAAEQQKVAENAAAKAKEQQKV
AEAAAAKAKAEADKVAKAQADAQKKAESEAKKEAAAAATAKKQAEEAKKAAAAEAAEKKAAADAEKKAAAEAEKAEKKAA
ADAEKKAAAEAEKKAAAAEKAAAKKAADAKKKAAAAAAEQSSEVDDLFGGLADGKNAPKGGGAKAKGDGKPAGQGNAKTA
GDKGVDSSGYEGQIRTAIQNKFYDASSFAGKTCDLRIKLGPDGFLIDVKAVGGDPALCQAAVAAANQARIPKPPSDAVYQ
KVKNFTLVFKPQ

Specific function: Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA [H]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786960, Length=125, Percent_Identity=62.4, Blast_Score=161, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014161 [H]

Pfam domain/function: PF06519 TolA [H]

EC number: NA

Molecular weight: Translated: 42907; Mature: 42907

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEECCC
>Mature Secondary Structure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CCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2687247; 8905232; 9278503; 2068069; 8978668; 10404600 [H]