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The map label for this gene is phrB [H]

Identifier: 157369495

GI number: 157369495

Start: 1377858

End: 1379288

Strand: Direct

Name: phrB [H]

Synonym: Spro_1252

Alternate gene names: 157369495

Gene position: 1377858-1379288 (Clockwise)

Preceding gene: 157369494

Following gene: 157369496

Centisome position: 25.29

GC content: 57.44

Gene sequence:

>1431_bases
ATGACCACGCATCTGGTCTGGTTGCGTAACGATCTGCGTATTACCGACAACAAAGCGTTGCATGCCGCTTGCAGCGATCC
CGACGCCCGCGTGCTGGCGGTGTTTATCGCCACCCCGCAACAATGGCGACAGCACGAAATGGCCCCCCGTCAGGTTGCAT
TTATCCATGCCAACCTGCTGCAGGTGCAGCAGGCTCTTGCCGAACGCGGCATTCCGCTTGGCTACCACCAGTGTGATGAT
TTTGCCGCCGCGGTTGACTGGCTGACGGCGTATTGCGCGCAGCAGCAGGTGGATAGGCTGTTTTACAATCGCCAGTATGA
AATCAATGAGCGTCAGCGCGACCAACGGCTGGAACAGCGCTTGTCCGGGCAGGCGATTTGCCAGAGTTTTGATGACAGCC
TGCTGTTACCGCCGGGTAGCGTGCAGACCGGTAGCGGCGAAATGTACAAAATCTACACGCCATTTCGTAAAGCCTTTATC
CAGCGTCTGACTGAATCCGACATTCAATCCTTGCCTGCGCCAAAACCACGGGCAGGCGGCGCATTACCGGTAACCGCTAT
CCCAGAGGCATTTGATTATCCGCCGGCAGAGCCGAACGAGGATTTCCCCGCCGGGGAAGAGGCGGCGCTACAGCGGCTAC
GCAGCTTTTGCCGTGAGCAGGTGCAGGATTACCTCGAGCAGCGGGATTTGCCCGCCATTGCCGGGACCAGCAGCCTGTCG
CCTTATCTGGCAATTGGCGTTTTGTCGCCGCGCCAATGTTTTAACCGCCTGCGAGCTGAATGTCCGCAACTGCTGGAAAA
TCATGACAGCGGTGCCTTCGGCTGGCTCAATGAGCTGATCTGGCGCGAGTTTTATCGGCATTTGATGGTGGCCTATCCCG
CGTTATGCAAGCACCGTCCCTTTATCGAATGGACGGATAGGGTACGCTGGCAGGATAACCAAGAACTGTTGCAGGCCTGG
CAGCAGGGCGTGACCGGTTATCCGATTGTCGATGCAGCCATGCGCCAATTGAATACCACCGGCTGGATGCACAACCGATT
ACGCATGATCAGCGCCAGCTTTCTGGTGAAAGACTTGTTGATCGACTGGCGTGCCGGTGAAAGCTATTTCATGTCACAGC
TATTGGACGGCGATCTGGCGGCCAATAATGGCGGTTGGCAGTGGGCGGCCTCAACCGGCACTGACGCTGCGCCGTACTTC
CGCATCTTTAACCCGACCACTCAGGGCGAACGTTTTGATCCGCAAGGCACTTTTATCCGTAAATGGTTGTCCGAGCTGGC
GGATGTACCGGACAATGCTATCCATCAACCCCATCGCTGGGCAGAACAACAGCAGCGCGTGCTGGATTATCCGCTGCCGA
TCGTCGACCACAAACAGGCGCGGCTGGAGACGCTGGCGGCCTTTGAAGCGGCGAAGCGCGGGGAATATTAA

Upstream 100 bases:

>100_bases
GGCCGAATACCCCGATCGCTATCTGGCCGATCAGCGTTATTTCGAGCCCTATCCCGAGGCGTTGCGCCTGCGTTATGGCC
ATTAATTGACAAGGAGTTTT

Downstream 100 bases:

>100_bases
GGCAACAGCAAGGAGCGAAATAATGATGAAAAAGGTGCTGGCCCTGTGCCTGAGCGGCTATTGCGCCTTGGCGCAGGCCG
GCTTTGACGTGGTGGCGCTG

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTTHLVWLRNDLRITDNKALHAACSDPDARVLAVFIATPQQWRQHEMAPRQVAFIHANLLQVQQALAERGIPLGYHQCDD
FAAAVDWLTAYCAQQQVDRLFYNRQYEINERQRDQRLEQRLSGQAICQSFDDSLLLPPGSVQTGSGEMYKIYTPFRKAFI
QRLTESDIQSLPAPKPRAGGALPVTAIPEAFDYPPAEPNEDFPAGEEAALQRLRSFCREQVQDYLEQRDLPAIAGTSSLS
PYLAIGVLSPRQCFNRLRAECPQLLENHDSGAFGWLNELIWREFYRHLMVAYPALCKHRPFIEWTDRVRWQDNQELLQAW
QQGVTGYPIVDAAMRQLNTTGWMHNRLRMISASFLVKDLLIDWRAGESYFMSQLLDGDLAANNGGWQWAASTGTDAAPYF
RIFNPTTQGERFDPQGTFIRKWLSELADVPDNAIHQPHRWAEQQQRVLDYPLPIVDHKQARLETLAAFEAAKRGEY

Sequences:

>Translated_476_residues
MTTHLVWLRNDLRITDNKALHAACSDPDARVLAVFIATPQQWRQHEMAPRQVAFIHANLLQVQQALAERGIPLGYHQCDD
FAAAVDWLTAYCAQQQVDRLFYNRQYEINERQRDQRLEQRLSGQAICQSFDDSLLLPPGSVQTGSGEMYKIYTPFRKAFI
QRLTESDIQSLPAPKPRAGGALPVTAIPEAFDYPPAEPNEDFPAGEEAALQRLRSFCREQVQDYLEQRDLPAIAGTSSLS
PYLAIGVLSPRQCFNRLRAECPQLLENHDSGAFGWLNELIWREFYRHLMVAYPALCKHRPFIEWTDRVRWQDNQELLQAW
QQGVTGYPIVDAAMRQLNTTGWMHNRLRMISASFLVKDLLIDWRAGESYFMSQLLDGDLAANNGGWQWAASTGTDAAPYF
RIFNPTTQGERFDPQGTFIRKWLSELADVPDNAIHQPHRWAEQQQRVLDYPLPIVDHKQARLETLAAFEAAKRGEY
>Mature_475_residues
TTHLVWLRNDLRITDNKALHAACSDPDARVLAVFIATPQQWRQHEMAPRQVAFIHANLLQVQQALAERGIPLGYHQCDDF
AAAVDWLTAYCAQQQVDRLFYNRQYEINERQRDQRLEQRLSGQAICQSFDDSLLLPPGSVQTGSGEMYKIYTPFRKAFIQ
RLTESDIQSLPAPKPRAGGALPVTAIPEAFDYPPAEPNEDFPAGEEAALQRLRSFCREQVQDYLEQRDLPAIAGTSSLSP
YLAIGVLSPRQCFNRLRAECPQLLENHDSGAFGWLNELIWREFYRHLMVAYPALCKHRPFIEWTDRVRWQDNQELLQAWQ
QGVTGYPIVDAAMRQLNTTGWMHNRLRMISASFLVKDLLIDWRAGESYFMSQLLDGDLAANNGGWQWAASTGTDAAPYFR
IFNPTTQGERFDPQGTFIRKWLSELADVPDNAIHQPHRWAEQQQRVLDYPLPIVDHKQARLETLAAFEAAKRGEY

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=272, Percent_Identity=33.0882352941176, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI188536103, Length=270, Percent_Identity=31.4814814814815, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI188536100, Length=270, Percent_Identity=31.4814814814815, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI1786926, Length=478, Percent_Identity=61.2970711297071, Blast_Score=600, Evalue=1e-173,
Organism=Saccharomyces cerevisiae, GI6324962, Length=504, Percent_Identity=32.1428571428571, Blast_Score=265, Evalue=9e-72,
Organism=Drosophila melanogaster, GI17137248, Length=287, Percent_Identity=34.4947735191638, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24585455, Length=287, Percent_Identity=34.4947735191638, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24648152, Length=521, Percent_Identity=26.4875239923225, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54572; Mature: 54441

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHLVWLRNDLRITDNKALHAACSDPDARVLAVFIATPQQWRQHEMAPRQVAFIHANLL
CCCEEEEEECCCEECCCCCEEECCCCCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHHH
QVQQALAERGIPLGYHQCDDFAAAVDWLTAYCAQQQVDRLFYNRQYEINERQRDQRLEQR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LSGQAICQSFDDSLLLPPGSVQTGSGEMYKIYTPFRKAFIQRLTESDIQSLPAPKPRAGG
HHHHHHHHHCCCCEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCC
ALPVTAIPEAFDYPPAEPNEDFPAGEEAALQRLRSFCREQVQDYLEQRDLPAIAGTSSLS
CCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PYLAIGVLSPRQCFNRLRAECPQLLENHDSGAFGWLNELIWREFYRHLMVAYPALCKHRP
CHHEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FIEWTDRVRWQDNQELLQAWQQGVTGYPIVDAAMRQLNTTGWMHNRLRMISASFLVKDLL
CCCHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDWRAGESYFMSQLLDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGERFDPQGTFIR
HHCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
KWLSELADVPDNAIHQPHRWAEQQQRVLDYPLPIVDHKQARLETLAAFEAAKRGEY
HHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTHLVWLRNDLRITDNKALHAACSDPDARVLAVFIATPQQWRQHEMAPRQVAFIHANLL
CCEEEEEECCCEECCCCCEEECCCCCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHHH
QVQQALAERGIPLGYHQCDDFAAAVDWLTAYCAQQQVDRLFYNRQYEINERQRDQRLEQR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LSGQAICQSFDDSLLLPPGSVQTGSGEMYKIYTPFRKAFIQRLTESDIQSLPAPKPRAGG
HHHHHHHHHCCCCEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCC
ALPVTAIPEAFDYPPAEPNEDFPAGEEAALQRLRSFCREQVQDYLEQRDLPAIAGTSSLS
CCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PYLAIGVLSPRQCFNRLRAECPQLLENHDSGAFGWLNELIWREFYRHLMVAYPALCKHRP
CHHEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FIEWTDRVRWQDNQELLQAWQQGVTGYPIVDAAMRQLNTTGWMHNRLRMISASFLVKDLL
CCCHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDWRAGESYFMSQLLDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGERFDPQGTFIR
HHCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
KWLSELADVPDNAIHQPHRWAEQQQRVLDYPLPIVDHKQARLETLAAFEAAKRGEY
HHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840665; 11677609 [H]