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The map label for this gene is azoR [H]

Identifier: 157369452

GI number: 157369452

Start: 1326077

End: 1326775

Strand: Reverse

Name: azoR [H]

Synonym: Spro_1209

Alternate gene names: 157369452

Gene position: 1326775-1326077 (Counterclockwise)

Preceding gene: 157369453

Following gene: 157369450

Centisome position: 24.35

GC content: 54.51

Gene sequence:

>699_bases
ATGAGTCGTATACTGGTGCTGAAATCAAGTATCCTGGGCGATTACTCCCAGTCTGGAAAATTAGTCGATTTTTTTGTTCA
ACAGTGGCGTGAAGCACACCCAAGCGACAGTTTTACCTTCCGCGATCTGGCCAACCCGACCCTGCCGGAGCTGGATGGTG
AAGTGATTGGCGGCTTTAGCGCCGGTGATAAGCCCTTAACGCCACATCAGCAAAAAACGCTGGCGCTTTCCGATGAGCTG
ATTGCCGAGCTGAAATCGCACGACACCATTATCATCAGCGCCCCAATGTACAACTTCAATATCCCGACCCAGTTGAAAAT
CTATTTCGACCTGGTCGCGCGTGCCGGCCAAACCTTCCGTTATACCTCCGAGGGTTCCGAAGGCCTGGTCACCGGTAAAA
AAGCGATTGTGATCTCCAGCCGCGGCGGTATCCATGCCGAGACACCAACCGACCTGATCACGCCTTACCTGAAGCTGTTC
CTGGGGTTCATCGGTATTACTGACGTGGAGTTCGTGTTGGCGGAAGGTTTTGCTTACGGGCCGGAAGCGGCAGAAAAAGC
AACGCAGGACAGCCGCATAGCGGTTGCGCAGAAAGTGCCTGCGACGGTATACGCCGCCCTCGCAAACCAGCCTGAACTGG
CCACAGCGCCGGCCGGCGGCGGTTTCCTCAGTAACCTGATGAAAAAACTGTTCGGTTAA

Upstream 100 bases:

>100_bases
CAGCGAATTTTTACGCTATCACTGGCCCAGGCTTGCCTCTAAGATGCTTTAAACGCACAGCGAACGCTGACAACCTTAGA
GCAAGCCTGGAGATATCCGT

Downstream 100 bases:

>100_bases
TCACGATCTCCCTGCCCCGGCCCTTTCCGCACAGGAAAAGGCCAGGGTGCGGGAAAACTCAAGGTTCGAAAGGCCGACGC
TGAAACTGGTCATGGCCACA

Product: NAD(P)H dehydrogenase (quinone)

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSRILVLKSSILGDYSQSGKLVDFFVQQWREAHPSDSFTFRDLANPTLPELDGEVIGGFSAGDKPLTPHQQKTLALSDEL
IAELKSHDTIIISAPMYNFNIPTQLKIYFDLVARAGQTFRYTSEGSEGLVTGKKAIVISSRGGIHAETPTDLITPYLKLF
LGFIGITDVEFVLAEGFAYGPEAAEKATQDSRIAVAQKVPATVYAALANQPELATAPAGGGFLSNLMKKLFG

Sequences:

>Translated_232_residues
MSRILVLKSSILGDYSQSGKLVDFFVQQWREAHPSDSFTFRDLANPTLPELDGEVIGGFSAGDKPLTPHQQKTLALSDEL
IAELKSHDTIIISAPMYNFNIPTQLKIYFDLVARAGQTFRYTSEGSEGLVTGKKAIVISSRGGIHAETPTDLITPYLKLF
LGFIGITDVEFVLAEGFAYGPEAAEKATQDSRIAVAQKVPATVYAALANQPELATAPAGGGFLSNLMKKLFG
>Mature_231_residues
SRILVLKSSILGDYSQSGKLVDFFVQQWREAHPSDSFTFRDLANPTLPELDGEVIGGFSAGDKPLTPHQQKTLALSDELI
AELKSHDTIIISAPMYNFNIPTQLKIYFDLVARAGQTFRYTSEGSEGLVTGKKAIVISSRGGIHAETPTDLITPYLKLFL
GFIGITDVEFVLAEGFAYGPEAAEKATQDSRIAVAQKVPATVYAALANQPELATAPAGGGFLSNLMKKLFG

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=201, Percent_Identity=68.6567164179104, Blast_Score=287, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 25057; Mature: 24926

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRILVLKSSILGDYSQSGKLVDFFVQQWREAHPSDSFTFRDLANPTLPELDGEVIGGFS
CCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCCCEEECCC
AGDKPLTPHQQKTLALSDELIAELKSHDTIIISAPMYNFNIPTQLKIYFDLVARAGQTFR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCHHHHHHHHHHHCCCEEE
YTSEGSEGLVTGKKAIVISSRGGIHAETPTDLITPYLKLFLGFIGITDVEFVLAEGFAYG
ECCCCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
PEAAEKATQDSRIAVAQKVPATVYAALANQPELATAPAGGGFLSNLMKKLFG
CHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SRILVLKSSILGDYSQSGKLVDFFVQQWREAHPSDSFTFRDLANPTLPELDGEVIGGFS
CEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCCCEEECCC
AGDKPLTPHQQKTLALSDELIAELKSHDTIIISAPMYNFNIPTQLKIYFDLVARAGQTFR
CCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCHHHHHHHHHHHCCCEEE
YTSEGSEGLVTGKKAIVISSRGGIHAETPTDLITPYLKLFLGFIGITDVEFVLAEGFAYG
ECCCCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
PEAAEKATQDSRIAVAQKVPATVYAALANQPELATAPAGGGFLSNLMKKLFG
CHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA