Click here to switch to the map view.

The map label for this gene is rihA [H]

Identifier: 157369453

GI number: 157369453

Start: 1326924

End: 1327856

Strand: Reverse

Name: rihA [H]

Synonym: Spro_1210

Alternate gene names: 157369453

Gene position: 1327856-1326924 (Counterclockwise)

Preceding gene: 157369454

Following gene: 157369452

Centisome position: 24.37

GC content: 58.95

Gene sequence:

>933_bases
ATGCCTCGCCCTATCATTATCGATTGCGATCCCGGCCTCGACGACGCCATTGCCCTTGCTATGGCACTGAGTTCACCGGA
ACTTGAAGTGAAAGCCATTACCACCTCTGCCGGTAACCAGACGCCGGAGAAAACCCTGCATAACGCGCTCGGTCTGCTGA
CGCTGATGAAACGCGAAGACATTCCTGTCGCCGCCGGTGCTGCGGCCCCGCTAATGCGTGAGCTGGTGATTGCCGACTAT
GTGCATGGCAAAACCGGCATGGGCAATACCCATCTGCCAACGCCCACCATCAAGCCGGTAAAACAAAGCGCCGTCGAACT
GATCGCCAGCCTGTTGCGCAGCAGCCCGCAACCCATCACTCTGGTGGTTACCGGTCCAATGACCAATATCGCCCTGCTGC
TGGCTCAACACCCTGAGCTGAAAGGCAACATCGAGCGTATCGTCTTTATGGGCGGCGGCATGAACGCCGGCAACACCACC
CCGGTGGCGGAATTTAATATTTTCGTCGACCCGGAAGCCGCCGAAATGGTACTCAAATCCGGCGTGCCGCTGACCATGGC
GGGTCTGAACGTCACCCATCAGGCGCTGGTACTGCCGCAGGATATCGAACGAATCCGCCAGATTGACAACCCGGTCGCGC
AGGCGGTAGCAGAAATGCTCGACTTCTACCTGCCGCTGTATCTCAGCCACCCGCGCGGATTGCCCGGCGCCGCGATGCAC
GACCCCTGCACCATCGCCTGGCTGTTGGCACCACGGCTGTTTGGCAGTATCGAACGCTGGGTCGGTGTAGAGACCCAAGG
AGAGTACACCCAGGGGATGACGGTGGTGGATATCTTCGGACAAAACGGCAAAGCGGCCAACGTGGAAGTGCTGACCGATA
TCGATCGTGAAGGGTTTATCAATCTGCTGGCCGAACGCGTCGCGCGTTACTGA

Upstream 100 bases:

>100_bases
CGCTCCATTCGGGGCGCATCGCCCCTCTCCCGGCACTAAAAGCGCTTGTTTCTCCCTGGTTTTGATCCGATGCTCGGAAC
ACAACTACAAGGAGATCTCT

Downstream 100 bases:

>100_bases
CCCGCTGGGACACCAACATAGTCAGCATTCAGTTTTTTTGATTTAACTCAGCGAATTTTTACGCTATCACTGGCCCAGGC
TTGCCTCTAAGATGCTTTAA

Product: ribonucleoside hydrolase 1

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPRPIIIDCDPGLDDAIALAMALSSPELEVKAITTSAGNQTPEKTLHNALGLLTLMKREDIPVAAGAAAPLMRELVIADY
VHGKTGMGNTHLPTPTIKPVKQSAVELIASLLRSSPQPITLVVTGPMTNIALLLAQHPELKGNIERIVFMGGGMNAGNTT
PVAEFNIFVDPEAAEMVLKSGVPLTMAGLNVTHQALVLPQDIERIRQIDNPVAQAVAEMLDFYLPLYLSHPRGLPGAAMH
DPCTIAWLLAPRLFGSIERWVGVETQGEYTQGMTVVDIFGQNGKAANVEVLTDIDREGFINLLAERVARY

Sequences:

>Translated_310_residues
MPRPIIIDCDPGLDDAIALAMALSSPELEVKAITTSAGNQTPEKTLHNALGLLTLMKREDIPVAAGAAAPLMRELVIADY
VHGKTGMGNTHLPTPTIKPVKQSAVELIASLLRSSPQPITLVVTGPMTNIALLLAQHPELKGNIERIVFMGGGMNAGNTT
PVAEFNIFVDPEAAEMVLKSGVPLTMAGLNVTHQALVLPQDIERIRQIDNPVAQAVAEMLDFYLPLYLSHPRGLPGAAMH
DPCTIAWLLAPRLFGSIERWVGVETQGEYTQGMTVVDIFGQNGKAANVEVLTDIDREGFINLLAERVARY
>Mature_309_residues
PRPIIIDCDPGLDDAIALAMALSSPELEVKAITTSAGNQTPEKTLHNALGLLTLMKREDIPVAAGAAAPLMRELVIADYV
HGKTGMGNTHLPTPTIKPVKQSAVELIASLLRSSPQPITLVVTGPMTNIALLLAQHPELKGNIERIVFMGGGMNAGNTTP
VAEFNIFVDPEAAEMVLKSGVPLTMAGLNVTHQALVLPQDIERIRQIDNPVAQAVAEMLDFYLPLYLSHPRGLPGAAMHD
PCTIAWLLAPRLFGSIERWVGVETQGEYTQGMTVVDIFGQNGKAANVEVLTDIDREGFINLLAERVARY

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=310, Percent_Identity=61.2903225806452, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1786213, Length=308, Percent_Identity=46.7532467532467, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1788486, Length=308, Percent_Identity=39.9350649350649, Blast_Score=212, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32565474, Length=194, Percent_Identity=34.020618556701, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565698, Length=195, Percent_Identity=31.7948717948718, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320608, Length=300, Percent_Identity=32, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24641837, Length=334, Percent_Identity=30.8383233532934, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24583915, Length=332, Percent_Identity=26.5060240963855, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24641839, Length=325, Percent_Identity=27.6923076923077, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33303; Mature: 33172

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPIIIDCDPGLDDAIALAMALSSPELEVKAITTSAGNQTPEKTLHNALGLLTLMKRED
CCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
IPVAAGAAAPLMRELVIADYVHGKTGMGNTHLPTPTIKPVKQSAVELIASLLRSSPQPIT
CCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
LVVTGPMTNIALLLAQHPELKGNIERIVFMGGGMNAGNTTPVAEFNIFVDPEAAEMVLKS
EEEECCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEECCHHHHHHHHC
GVPLTMAGLNVTHQALVLPQDIERIRQIDNPVAQAVAEMLDFYLPLYLSHPRGLPGAAMH
CCCEEEECCCCCHHEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DPCTIAWLLAPRLFGSIERWVGVETQGEYTQGMTVVDIFGQNGKAANVEVLTDIDREGFI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEEEECCCCCCCCEEEEECCCHHHHH
NLLAERVARY
HHHHHHHHCC
>Mature Secondary Structure 
PRPIIIDCDPGLDDAIALAMALSSPELEVKAITTSAGNQTPEKTLHNALGLLTLMKRED
CCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
IPVAAGAAAPLMRELVIADYVHGKTGMGNTHLPTPTIKPVKQSAVELIASLLRSSPQPIT
CCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
LVVTGPMTNIALLLAQHPELKGNIERIVFMGGGMNAGNTTPVAEFNIFVDPEAAEMVLKS
EEEECCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEECCHHHHHHHHC
GVPLTMAGLNVTHQALVLPQDIERIRQIDNPVAQAVAEMLDFYLPLYLSHPRGLPGAAMH
CCCEEEECCCCCHHEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DPCTIAWLLAPRLFGSIERWVGVETQGEYTQGMTVVDIFGQNGKAANVEVLTDIDREGFI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEEEECCCCCCCCEEEEECCCHHHHH
NLLAERVARY
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA