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The map label for this gene is mhpC [H]

Identifier: 157369233

GI number: 157369233

Start: 1094256

End: 1095152

Strand: Reverse

Name: mhpC [H]

Synonym: Spro_0990

Alternate gene names: 157369233

Gene position: 1095152-1094256 (Counterclockwise)

Preceding gene: 157369235

Following gene: 157369232

Centisome position: 20.1

GC content: 54.52

Gene sequence:

>897_bases
ATGAGTATGGTTTGCGTACAACACGGCAACCGGATAATCAACATGAACGGCTTAACATCTGTGCTTAATCAGCAGGTTAT
TGTTAATGGCCATCGTATTGCCACCGGCATACATGGCACCGGCAACCCCCTGGTATTGGTACACGGCACCCCTGCCCACT
CAATCATCTGGCGGGATTTGCTGCCCAGCCTGACGGCGGCGGGCTTTAGGGTCCATATCTACGATTTATTGGGTTACGGC
GCATCAGAACGCCCATTGTCGGCTGACACTTCAATTGCTGCACAGGCAGATTTACTGATCGACCTGCTGGACCATTGGCA
ACTGGAGTCCACCCACGTGTTTGGTCACGATATTGGGGGCGCATTGTCGCTACGCGCAGCGTTTGGGCACTCCCGGCGCT
TTCGCTCGCTGACCATCGCGGATATTTGCAGTTATGATTCCTGGCCTTCGCCAAGCTGGCGCGGCATCCGCGATAATTAT
CATCAGTACGCCGTTATGGACGCCTCACAGCATGAGCAAACCCTGGCTCGCCAACTTAAAATGGCGGTGTTCAACAAATC
GCGGATGTCCGGAGAGCTATTACAGCACTATCTGGCACCGATCGTCGGCGTGGTCGGTCAACCGGCATTTTATCAACATC
AAATCGCCCATTACGACGCCCGCTATACTGAAGACTTCGCCCAGCGCCTGCCTGAACTACGTCTGCCGGTGCAAATCCTG
TGGGGTGAGAACGATGAGTGGCAACCGGTCAGTTACGCCTATCGTTTGCAGGGCGACATTCCCGGTGCCAGGCTGCAGGT
GATCCCTCAGGCGGGCCATTTTTTAATGGAAGACGCGCCGGAAACGGTAGCCCAACGGCTGGTGACGTTTATTCATTCAC
TTAAATCGGGCAAATAG

Upstream 100 bases:

>100_bases
CGCCCGTAAAGCATCAATGGATAAGCGCTGGTTCATAGACACCTCATGACAAAAATCGGATTTACTCACCATAACTTGTC
ATTGGCGTCATAGGAAGACG

Downstream 100 bases:

>100_bases
GCTCAGGAGAACGTGATGCAACTGATTGGCATGCTGGATTCACCCTATGTACGCCGGGTGGCAATTTGTCTGAAATTATT
AGAGATAGAGTTTGAGCACC

Product: alpha/beta hydrolase fold domain-containing protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSMVCVQHGNRIINMNGLTSVLNQQVIVNGHRIATGIHGTGNPLVLVHGTPAHSIIWRDLLPSLTAAGFRVHIYDLLGYG
ASERPLSADTSIAAQADLLIDLLDHWQLESTHVFGHDIGGALSLRAAFGHSRRFRSLTIADICSYDSWPSPSWRGIRDNY
HQYAVMDASQHEQTLARQLKMAVFNKSRMSGELLQHYLAPIVGVVGQPAFYQHQIAHYDARYTEDFAQRLPELRLPVQIL
WGENDEWQPVSYAYRLQGDIPGARLQVIPQAGHFLMEDAPETVAQRLVTFIHSLKSGK

Sequences:

>Translated_298_residues
MSMVCVQHGNRIINMNGLTSVLNQQVIVNGHRIATGIHGTGNPLVLVHGTPAHSIIWRDLLPSLTAAGFRVHIYDLLGYG
ASERPLSADTSIAAQADLLIDLLDHWQLESTHVFGHDIGGALSLRAAFGHSRRFRSLTIADICSYDSWPSPSWRGIRDNY
HQYAVMDASQHEQTLARQLKMAVFNKSRMSGELLQHYLAPIVGVVGQPAFYQHQIAHYDARYTEDFAQRLPELRLPVQIL
WGENDEWQPVSYAYRLQGDIPGARLQVIPQAGHFLMEDAPETVAQRLVTFIHSLKSGK
>Mature_297_residues
SMVCVQHGNRIINMNGLTSVLNQQVIVNGHRIATGIHGTGNPLVLVHGTPAHSIIWRDLLPSLTAAGFRVHIYDLLGYGA
SERPLSADTSIAAQADLLIDLLDHWQLESTHVFGHDIGGALSLRAAFGHSRRFRSLTIADICSYDSWPSPSWRGIRDNYH
QYAVMDASQHEQTLARQLKMAVFNKSRMSGELLQHYLAPIVGVVGQPAFYQHQIAHYDARYTEDFAQRLPELRLPVQILW
GENDEWQPVSYAYRLQGDIPGARLQVIPQAGHFLMEDAPETVAQRLVTFIHSLKSGK

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MhpC family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=257, Percent_Identity=21.4007782101167, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33314; Mature: 33183

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMVCVQHGNRIINMNGLTSVLNQQVIVNGHRIATGIHGTGNPLVLVHGTPAHSIIWRDL
CCEEEEECCCEEEEECHHHHHHCCCEEECCEEEEEECCCCCCCEEEEECCCHHHHHHHHH
LPSLTAAGFRVHIYDLLGYGASERPLSADTSIAAQADLLIDLLDHWQLESTHVFGHDIGG
HHHHHHCCEEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCC
ALSLRAAFGHSRRFRSLTIADICSYDSWPSPSWRGIRDNYHQYAVMDASQHEQTLARQLK
CEEHHHHHCCCHHHHEEHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
MAVFNKSRMSGELLQHYLAPIVGVVGQPAFYQHQIAHYDARYTEDFAQRLPELRLPVQIL
HHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
WGENDEWQPVSYAYRLQGDIPGARLQVIPQAGHFLMEDAPETVAQRLVTFIHSLKSGK
ECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SMVCVQHGNRIINMNGLTSVLNQQVIVNGHRIATGIHGTGNPLVLVHGTPAHSIIWRDL
CEEEEECCCEEEEECHHHHHHCCCEEECCEEEEEECCCCCCCEEEEECCCHHHHHHHHH
LPSLTAAGFRVHIYDLLGYGASERPLSADTSIAAQADLLIDLLDHWQLESTHVFGHDIGG
HHHHHHCCEEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCC
ALSLRAAFGHSRRFRSLTIADICSYDSWPSPSWRGIRDNYHQYAVMDASQHEQTLARQLK
CEEHHHHHCCCHHHHEEHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
MAVFNKSRMSGELLQHYLAPIVGVVGQPAFYQHQIAHYDARYTEDFAQRLPELRLPVQIL
HHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
WGENDEWQPVSYAYRLQGDIPGARLQVIPQAGHFLMEDAPETVAQRLVTFIHSLKSGK
ECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA