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The map label for this gene is ipdC [H]

Identifier: 157369235

GI number: 157369235

Start: 1096106

End: 1097782

Strand: Reverse

Name: ipdC [H]

Synonym: Spro_0992

Alternate gene names: 157369235

Gene position: 1097782-1096106 (Counterclockwise)

Preceding gene: 157369239

Following gene: 157369233

Centisome position: 20.15

GC content: 55.52

Gene sequence:

>1677_bases
ATGAAAATCACTATTGGAGCCTTTATCCTGCAACAGCTCCGCACCCTGAACATCGATCGTATTTATGGCGTACCCGGCGA
CTATAATCTGGCGCTGCTGGAACTGATTGAACAGGATGACAAGCTGGAGTTCATTGGCAACTGTAATGAACTCAATGCCT
CCTACGCAGCCGATGGCTATGCACGGCTAAAAGGCGCCGGAGCGCTAATCACTACCTATGGCGTCGGCGACCTGGCCGCA
CTGTCCGGCATTGCCGGTGCCTACGCCGAATCGGCCCCGGTGGTCTGCCTGTCCGGTACCCCGCCGCTGCATGCCATGAA
AAATCACCTGCTGCTGCACCACACGCTGGCGGACGGTAACTTTGATAACGTGATGAACTGTTTTAAACAATTCACCGTCG
CCCAGGCATTAATTACGCCGGAAAATGCGGCAGTGGAAATTCCCCGCGTTATTTCCCGCGCCTGGACGGAAAAGAAACCG
GTTTATTTGCAATTACCTTCTGATATTTGTGATGTAGAAATTGAAATCACTCAGGCGGTGGCACCACCGCAATTACCCGC
CAGCGACAAATATAATGTTCAACTGGCGGCAATGGCGTTGCTTGCGCGGCTAAAACTGGCAAAAAAACCGATTATTCTGG
TCGACCAAATGGTGGACCGCTTTCAGCTACAACAATTAGTGATCGACGTGGCGCATAAATTCGCCATCCCGCTCACCAAC
ATGCCGACGGCCAAATGCATTATTCCAGAAACCACGCCGGGCTGGATCGGCGGTTACAGCGGTAATCTGTCACGCCCCGA
ACTCTATGAACACATGGCGCACTCAGACTGCGTGCTGAGCTTCGGCGTACGGTTGGTGGACTCCACCACCGGCTATTTCT
CGCATCGCATCCCGTCCGACGCGCAAATCGATATTCAGCCGTTTTCAGTGAAGCTGGGAGAAACTTCTTATCCGGCAGTG
GCGACGGCAGACCTGCTACAGGCGCTGCTGGCATTGGGTGAAGGCGTGGCAGATCATCAGCCGGCCGTACTACCCGAAGC
GCGCCATCGGCTGGCGACGCCGAGCGAAACCCCAATCGATCAGGCTTATTTATGGCAGCGCCTGCAACGCTTTATTCGGG
CCGATGACGTGCTGGTGGTAGAGAACGGCACCTCGGGCGCGGCGATTGGCGGCATGCGGATGCCGGACGGCGTCCAGGTG
GTCAATCAGCCGATTTGGGGCTCGATCGGTTATACCTTACCGGCGCTGCTGGGCACCATGATGGCCGCCCCGGAACGGCG
GCACCTGCTGTTTATTGGCGATGGCTCGTTCCAGCTTACCGCGCAGGAAGTTTCCACCCTGCTGCGTTACGATCAGAAGC
CGATTATCTTCCTGATTAATAACGACGGTTACACCATCGAGCGTTACATTCTCGGCGAAAATTCGTCATACAACGATATT
GGGCCGTGGGACTACGCCAAGCTGCCTGCCGTGCTCAACACCCAGGCGACACCGTTTTGCGTGGCGGTAGAAACCACCCA
ACAGCTGGAGTTGGCGCTCGAGCACGCCTCACGACAGGATCAATTGACCTTTATCGAAATAAAGGTGCCGATGATGGACA
CACCGCCGGTGATGAAGGAGTTCTGCAACCGCTGCAATAACTTTAACTTCGGCCTGACTAACCCGCGCCGCGCTTAG

Upstream 100 bases:

>100_bases
GGGTTCAGCGTTATTTCATGATTCGTTACACAACATCACCGGCAATTAACTTTGCCGTTCTCTGCCTGATAAAAGAAAGA
TAAGAAATCCGGAGAAATAC

Downstream 100 bases:

>100_bases
CTCCATCCGCCGTTGCTCAGGTAACGGCAGATTTACCCAAAACCTGCACCACCTGCTGCTGCAAGGTTTGCAGTATTGGG
TGGTGCCGCCTGGCCCCCAG

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: Indolepyruvate decarboxylase [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MKITIGAFILQQLRTLNIDRIYGVPGDYNLALLELIEQDDKLEFIGNCNELNASYAADGYARLKGAGALITTYGVGDLAA
LSGIAGAYAESAPVVCLSGTPPLHAMKNHLLLHHTLADGNFDNVMNCFKQFTVAQALITPENAAVEIPRVISRAWTEKKP
VYLQLPSDICDVEIEITQAVAPPQLPASDKYNVQLAAMALLARLKLAKKPIILVDQMVDRFQLQQLVIDVAHKFAIPLTN
MPTAKCIIPETTPGWIGGYSGNLSRPELYEHMAHSDCVLSFGVRLVDSTTGYFSHRIPSDAQIDIQPFSVKLGETSYPAV
ATADLLQALLALGEGVADHQPAVLPEARHRLATPSETPIDQAYLWQRLQRFIRADDVLVVENGTSGAAIGGMRMPDGVQV
VNQPIWGSIGYTLPALLGTMMAAPERRHLLFIGDGSFQLTAQEVSTLLRYDQKPIIFLINNDGYTIERYILGENSSYNDI
GPWDYAKLPAVLNTQATPFCVAVETTQQLELALEHASRQDQLTFIEIKVPMMDTPPVMKEFCNRCNNFNFGLTNPRRA

Sequences:

>Translated_558_residues
MKITIGAFILQQLRTLNIDRIYGVPGDYNLALLELIEQDDKLEFIGNCNELNASYAADGYARLKGAGALITTYGVGDLAA
LSGIAGAYAESAPVVCLSGTPPLHAMKNHLLLHHTLADGNFDNVMNCFKQFTVAQALITPENAAVEIPRVISRAWTEKKP
VYLQLPSDICDVEIEITQAVAPPQLPASDKYNVQLAAMALLARLKLAKKPIILVDQMVDRFQLQQLVIDVAHKFAIPLTN
MPTAKCIIPETTPGWIGGYSGNLSRPELYEHMAHSDCVLSFGVRLVDSTTGYFSHRIPSDAQIDIQPFSVKLGETSYPAV
ATADLLQALLALGEGVADHQPAVLPEARHRLATPSETPIDQAYLWQRLQRFIRADDVLVVENGTSGAAIGGMRMPDGVQV
VNQPIWGSIGYTLPALLGTMMAAPERRHLLFIGDGSFQLTAQEVSTLLRYDQKPIIFLINNDGYTIERYILGENSSYNDI
GPWDYAKLPAVLNTQATPFCVAVETTQQLELALEHASRQDQLTFIEIKVPMMDTPPVMKEFCNRCNNFNFGLTNPRRA
>Mature_558_residues
MKITIGAFILQQLRTLNIDRIYGVPGDYNLALLELIEQDDKLEFIGNCNELNASYAADGYARLKGAGALITTYGVGDLAA
LSGIAGAYAESAPVVCLSGTPPLHAMKNHLLLHHTLADGNFDNVMNCFKQFTVAQALITPENAAVEIPRVISRAWTEKKP
VYLQLPSDICDVEIEITQAVAPPQLPASDKYNVQLAAMALLARLKLAKKPIILVDQMVDRFQLQQLVIDVAHKFAIPLTN
MPTAKCIIPETTPGWIGGYSGNLSRPELYEHMAHSDCVLSFGVRLVDSTTGYFSHRIPSDAQIDIQPFSVKLGETSYPAV
ATADLLQALLALGEGVADHQPAVLPEARHRLATPSETPIDQAYLWQRLQRFIRADDVLVVENGTSGAAIGGMRMPDGVQV
VNQPIWGSIGYTLPALLGTMMAAPERRHLLFIGDGSFQLTAQEVSTLLRYDQKPIIFLINNDGYTIERYILGENSSYNDI
GPWDYAKLPAVLNTQATPFCVAVETTQQLELALEHASRQDQLTFIEIKVPMMDTPPVMKEFCNRCNNFNFGLTNPRRA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG3961

COG function: function code GHR; Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=482, Percent_Identity=24.2738589211618, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=497, Percent_Identity=24.5472837022133, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531301, Length=497, Percent_Identity=24.748490945674, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=547, Percent_Identity=37.4771480804388, Blast_Score=355, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6323073, Length=551, Percent_Identity=36.1161524500907, Blast_Score=348, Evalue=9e-97,
Organism=Saccharomyces cerevisiae, GI6321524, Length=551, Percent_Identity=35.2087114337568, Blast_Score=328, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6320123, Length=560, Percent_Identity=32.1428571428571, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6320588, Length=598, Percent_Identity=28.7625418060201, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6323755, Length=313, Percent_Identity=22.6837060702875, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017764
- InterPro:   IPR012110
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.74 [H]

Molecular weight: Translated: 61287; Mature: 61287

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITIGAFILQQLRTLNIDRIYGVPGDYNLALLELIEQDDKLEFIGNCNELNASYAADGY
CEEEHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCH
ARLKGAGALITTYGVGDLAALSGIAGAYAESAPVVCLSGTPPLHAMKNHLLLHHTLADGN
HHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCEEEEEEECCCC
FDNVMNCFKQFTVAQALITPENAAVEIPRVISRAWTEKKPVYLQLPSDICDVEIEITQAV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECC
APPQLPASDKYNVQLAAMALLARLKLAKKPIILVDQMVDRFQLQQLVIDVAHKFAIPLTN
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
MPTAKCIIPETTPGWIGGYSGNLSRPELYEHMAHSDCVLSFGVRLVDSTTGYFSHRIPSD
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHCCCCCC
AQIDIQPFSVKLGETSYPAVATADLLQALLALGEGVADHQPAVLPEARHRLATPSETPID
CEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCH
QAYLWQRLQRFIRADDVLVVENGTSGAAIGGMRMPDGVQVVNQPIWGSIGYTLPALLGTM
HHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHH
MAAPERRHLLFIGDGSFQLTAQEVSTLLRYDQKPIIFLINNDGYTIERYILGENSSYNDI
HCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCC
GPWDYAKLPAVLNTQATPFCVAVETTQQLELALEHASRQDQLTFIEIKVPMMDTPPVMKE
CCCCHHHCCHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHH
FCNRCNNFNFGLTNPRRA
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKITIGAFILQQLRTLNIDRIYGVPGDYNLALLELIEQDDKLEFIGNCNELNASYAADGY
CEEEHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCH
ARLKGAGALITTYGVGDLAALSGIAGAYAESAPVVCLSGTPPLHAMKNHLLLHHTLADGN
HHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCEEEEEEECCCC
FDNVMNCFKQFTVAQALITPENAAVEIPRVISRAWTEKKPVYLQLPSDICDVEIEITQAV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECC
APPQLPASDKYNVQLAAMALLARLKLAKKPIILVDQMVDRFQLQQLVIDVAHKFAIPLTN
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
MPTAKCIIPETTPGWIGGYSGNLSRPELYEHMAHSDCVLSFGVRLVDSTTGYFSHRIPSD
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHCCCCCC
AQIDIQPFSVKLGETSYPAVATADLLQALLALGEGVADHQPAVLPEARHRLATPSETPID
CEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCH
QAYLWQRLQRFIRADDVLVVENGTSGAAIGGMRMPDGVQVVNQPIWGSIGYTLPALLGTM
HHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHH
MAAPERRHLLFIGDGSFQLTAQEVSTLLRYDQKPIIFLINNDGYTIERYILGENSSYNDI
HCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCC
GPWDYAKLPAVLNTQATPFCVAVETTQQLELALEHASRQDQLTFIEIKVPMMDTPPVMKE
CCCCHHHCCHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHH
FCNRCNNFNFGLTNPRRA
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034209 [H]