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The map label for this gene is chbR [H]

Identifier: 157369082

GI number: 157369082

Start: 933015

End: 933878

Strand: Direct

Name: chbR [H]

Synonym: Spro_0837

Alternate gene names: 157369082

Gene position: 933015-933878 (Clockwise)

Preceding gene: 157369081

Following gene: 157369083

Centisome position: 17.12

GC content: 48.84

Gene sequence:

>864_bases
ATGGGAGCGCGCGCGGTGTCTGACAACCTGGTTTCGCTGAATACCAACGACGTTAAGCTGATTCGGGAGCAGGATTTTTT
TAACTGCAAAGATTTCCATCTGTTTATCTATAACAAGGTGGAGAGCGCCACCGGGCTGCATCAGCATGACTATTACGAAT
TCACTATCGTCCTGAGCGGCAAATGCTATCAGGAGATCAACGGCAAGCGGGTGCTGCTGGAGCGGGGGGATTTTGTGTTT
ATCCCCATTGGCTCGCGTCACCAGAGCTTCTATGAGTTCGGCGCCGCGAAAATCTTTAACGTGGCGGTCGGCAAGGTTTT
CTTTGAAGAAAACTACCTGCAGCAACTGCCTCGCTGCTTTGTCGCCTCTCAGGCCTATAGCCTGAAGAATGAGTTTCTGG
CTTATATCGAGTCGGTGGTAGGTTCCCCACAGTTCCGTGAGGATGATTTTGCCGAGTTTCTGGAAACGCTGACCTTTTAT
ATCATCAGCCGCATTCGTCATTATAAAGAAGAGAGTGGCGAGGGGGATGATATACCTCAGTGGCTGAAGAACACCCTGGC
CGGCATGCATGATAAAGCGATGTTTGGTGAAAAGGCGTTGCTCAATATGGTTGAGCTGTCGGGGAAAACCCAGGAGTACC
TGACCCGGGCAATGCGGCGTTATTACCATAAAACGCCGATGCAGGTGATTAATGAAATTCGCATTAATTTCGCTAAAACC
CAGCTTGAGGTCACCAACTATTCGGTCTCGGATATTGCCTTTGATTCGGGCTATGGCGACGTGAGCCTGTTTATTAAAAA
CTTTAAAAGACTCACTGAAGTGACGCCGGGTAATTACCGAAAAAAGTTCTACGGCCCTATTTAG

Upstream 100 bases:

>100_bases
GCTACGGGTTAGTCCGGCTGGAGCGGGATAGCCAGCAACGTACGATTAAAAAGAGCGGCTATTGGTTTGCCGAGACGGCC
AGGCAAAACGGTTTTGATTA

Downstream 100 bases:

>100_bases
GGCCGCTCCCGATAAGCATTAACGCCGATATTCCGAATGCCTTTCAGGCCGCCAATAGCCTGATGAGATAAATTTCTTTG
CTTAAGCGAAGGGGAATTGC

Product: DNA-binding transcriptional regulator ChbR

Products: NA

Alternate protein names: Chb operon repressor [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MGARAVSDNLVSLNTNDVKLIREQDFFNCKDFHLFIYNKVESATGLHQHDYYEFTIVLSGKCYQEINGKRVLLERGDFVF
IPIGSRHQSFYEFGAAKIFNVAVGKVFFEENYLQQLPRCFVASQAYSLKNEFLAYIESVVGSPQFREDDFAEFLETLTFY
IISRIRHYKEESGEGDDIPQWLKNTLAGMHDKAMFGEKALLNMVELSGKTQEYLTRAMRRYYHKTPMQVINEIRINFAKT
QLEVTNYSVSDIAFDSGYGDVSLFIKNFKRLTEVTPGNYRKKFYGPI

Sequences:

>Translated_287_residues
MGARAVSDNLVSLNTNDVKLIREQDFFNCKDFHLFIYNKVESATGLHQHDYYEFTIVLSGKCYQEINGKRVLLERGDFVF
IPIGSRHQSFYEFGAAKIFNVAVGKVFFEENYLQQLPRCFVASQAYSLKNEFLAYIESVVGSPQFREDDFAEFLETLTFY
IISRIRHYKEESGEGDDIPQWLKNTLAGMHDKAMFGEKALLNMVELSGKTQEYLTRAMRRYYHKTPMQVINEIRINFAKT
QLEVTNYSVSDIAFDSGYGDVSLFIKNFKRLTEVTPGNYRKKFYGPI
>Mature_286_residues
GARAVSDNLVSLNTNDVKLIREQDFFNCKDFHLFIYNKVESATGLHQHDYYEFTIVLSGKCYQEINGKRVLLERGDFVFI
PIGSRHQSFYEFGAAKIFNVAVGKVFFEENYLQQLPRCFVASQAYSLKNEFLAYIESVVGSPQFREDDFAEFLETLTFYI
ISRIRHYKEESGEGDDIPQWLKNTLAGMHDKAMFGEKALLNMVELSGKTQEYLTRAMRRYYHKTPMQVINEIRINFAKTQ
LEVTNYSVSDIAFDSGYGDVSLFIKNFKRLTEVTPGNYRKKFYGPI

Specific function: Dual-function repressor/activator of the chbBCARFG operon. In the absence of the inducing sugar chitobiose, together with NagC, represses the chbBCARFG operon for the uptake and metabolism of chitobiose. In association with Crp, and probably in the presen

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788030, Length=271, Percent_Identity=65.3136531365314, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33322; Mature: 33191

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGARAVSDNLVSLNTNDVKLIREQDFFNCKDFHLFIYNKVESATGLHQHDYYEFTIVLSG
CCCCCCCCCEEEECCCCEEEEECCCCCCCCEEEEEEEEHHHHHCCCCCCCCEEEEEEECC
KCYQEINGKRVLLERGDFVFIPIGSRHQSFYEFGAAKIFNVAVGKVFFEENYLQQLPRCF
CHHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASQAYSLKNEFLAYIESVVGSPQFREDDFAEFLETLTFYIISRIRHYKEESGEGDDIPQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
WLKNTLAGMHDKAMFGEKALLNMVELSGKTQEYLTRAMRRYYHKTPMQVINEIRINFAKT
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHE
QLEVTNYSVSDIAFDSGYGDVSLFIKNFKRLTEVTPGNYRKKFYGPI
EEEEECCCHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GARAVSDNLVSLNTNDVKLIREQDFFNCKDFHLFIYNKVESATGLHQHDYYEFTIVLSG
CCCCCCCCEEEECCCCEEEEECCCCCCCCEEEEEEEEHHHHHCCCCCCCCEEEEEEECC
KCYQEINGKRVLLERGDFVFIPIGSRHQSFYEFGAAKIFNVAVGKVFFEENYLQQLPRCF
CHHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASQAYSLKNEFLAYIESVVGSPQFREDDFAEFLETLTFYIISRIRHYKEESGEGDDIPQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
WLKNTLAGMHDKAMFGEKALLNMVELSGKTQEYLTRAMRRYYHKTPMQVINEIRINFAKT
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHE
QLEVTNYSVSDIAFDSGYGDVSLFIKNFKRLTEVTPGNYRKKFYGPI
EEEEECCCHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 9405618 [H]