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The map label for this gene is rpoS [H]

Identifier: 157369077

GI number: 157369077

Start: 925441

End: 926439

Strand: Direct

Name: rpoS [H]

Synonym: Spro_0832

Alternate gene names: 157369077

Gene position: 925441-926439 (Clockwise)

Preceding gene: 157369076

Following gene: 157369079

Centisome position: 16.98

GC content: 53.05

Gene sequence:

>999_bases
ATGAGCCAAAATACGCTGAAAGTTAACGAGTTACATGACGATGCGGATTTCGACGAGAACGCAACGGAAGCGGAATCATT
CGACGAAAAAGCACTGGTTGAAGAAGAGGCCAGTGAGAGTGATTTAGCGGAAGAAGAGTTGTTGTCACAAGGCGTGACCC
AACGCGTATTGGATGCGACGCAGCTCTATCTTGGCGAAATCGGTTATTCTCCGCTGCTGACCGCAGAGGAAGAAGTTTAT
TTTGCGCGGCGTGCACTGCGCGGTGACGTGCCGTCCCGCCGCCGAATGATTGAAAGTAACCTGCGGTTGGTGGTGAAAAT
CGCCCGCCGTTACAGCAATCGCGGCCTGGCGCTGCTGGATCTGATTGAAGAGGGTAACCTCGGTCTGATCCGCGCAGTGG
AAAAGTTTGACCCAGAACGCGGTTTCCGTTTCTCGACTTACGCAACCTGGTGGATCCGGCAGACGATTGAACGGGCGATC
ATGAACCAAACCCGTACTATCCGCTTGCCTATCCACATTGTTAAAGAACTGAATGTCTATTTGCGCACCGCGCGCGAGCT
TTCTCATAAACTGGATCATGAACCGAGCGCTGAAGAGATTGCAGAGCAACTCGACAAGCCGGTCGATGACGTCAGCCGTA
TGCTGCGCCTGAATGAACGTATCACTTCTGTCGATACGCCATTGGGTGGGGATTCGGAGAAAGCCTTGCTGGACATTCTG
GCCGACGAGAAAGACAACGGACCAGAAGACACCACGCAAGACGATGACATGAAACAGAGCATCGTTAAATGGTTGTTCGA
ACTGAACGCCAAACAGCGTGAAGTGCTGGCGCGTCGTTTTGGCCTGCTGGGCTACGAAGCGGCAACGCTTGAAGATGTGG
GTCGTGAAATTGGCCTGACTCGCGAGCGAGTACGTCAGATCCAGGTTGAAGGTTTACGCCGTCTGCGTGAAATTCTGCAA
ATGCAGGGCCTGAGCATTGAGGCACTGTTCCGCGAGTAA

Upstream 100 bases:

>100_bases
ACAAGGGGAAATCCGTAAACCCGCTGCGTTATCTTCCGCAGCGATAGATTGGGCAGAATACGCTGTATTCTGCTCGCGGT
ATCACGGGTAGGAGCAGCAT

Downstream 100 bases:

>100_bases
CCCAGGCAGTTGAATCGCATCAAAATAAAAACGGTGAGCACTATGCTCACCGTTTTTTTATGCCTGCGACCTGCGAAGGG
TCAGACCATATTCTTCAGGC

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSQNTLKVNELHDDADFDENATEAESFDEKALVEEEASESDLAEEELLSQGVTQRVLDATQLYLGEIGYSPLLTAEEEVY
FARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAI
MNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
ADEKDNGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQ
MQGLSIEALFRE

Sequences:

>Translated_332_residues
MSQNTLKVNELHDDADFDENATEAESFDEKALVEEEASESDLAEEELLSQGVTQRVLDATQLYLGEIGYSPLLTAEEEVY
FARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAI
MNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
ADEKDNGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQ
MQGLSIEALFRE
>Mature_331_residues
SQNTLKVNELHDDADFDENATEAESFDEKALVEEEASESDLAEEELLSQGVTQRVLDATQLYLGEIGYSPLLTAEEEVYF
ARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIM
NQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILA
DEKDNGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQM
QGLSIEALFRE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=332, Percent_Identity=93.9759036144578, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1789448, Length=247, Percent_Identity=42.1052631578947, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1789871, Length=267, Percent_Identity=26.9662921348315, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38198; Mature: 38066

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNTLKVNELHDDADFDENATEAESFDEKALVEEEASESDLAEEELLSQGVTQRVLDAT
CCCCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QLYLGEIGYSPLLTAEEEVYFARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLD
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
LIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVY
HHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
LRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ADEKDNGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLT
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RERVRQIQVEGLRRLREILQMQGLSIEALFRE
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure 
SQNTLKVNELHDDADFDENATEAESFDEKALVEEEASESDLAEEELLSQGVTQRVLDAT
CCCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QLYLGEIGYSPLLTAEEEVYFARRALRGDVPSRRRMIESNLRLVVKIARRYSNRGLALLD
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
LIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVY
HHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
LRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDIL
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
ADEKDNGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLT
HCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RERVRQIQVEGLRRLREILQMQGLSIEALFRE
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]