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The map label for this gene is pcm

Identifier: 157369075

GI number: 157369075

Start: 923450

End: 924076

Strand: Direct

Name: pcm

Synonym: Spro_0830

Alternate gene names: 157369075

Gene position: 923450-924076 (Clockwise)

Preceding gene: 157369074

Following gene: 157369076

Centisome position: 16.95

GC content: 55.02

Gene sequence:

>627_bases
ATGGTGAACAAGCGTATGCAAACATTGCTGACGCAGCTGCGTCAACAGGGGATCCGGGACGAACGGTTGCTACAGGCGAT
CGAAGCAGTGCCGCGTGAACGCTTTGTTGATGAGGCACTTGAACACAAGGCTTATGAGAATACGGCGTTACCTATTGGCT
CCGGCCAAACTATCTCTCAACCTTATATGGTTGCACGCATGACCGAACTGCTGAATCTGACGCCAACCTCCAGAGTATTG
GAGATCGGCACCGGTTCTGGCTATCAAACCGCTATCCTGGCGCATCTGGTGCAGCATGTTTGTTCCGTTGAACGTATTAA
GGGGCTGCAATGGCAGGCCAAGCGGCGGCTGAAACAACTCGATCTTCACAATGTTTCCACTCGTCATGGCGATGGTTGGC
AGGGTTGGGCATCGCGAGGGCCGTTTGATGCCATCATTGTCACGGCAGCGCCGCCGGAGATCCCTCCGGCGCTGATGGAG
CAGTTGGACGACGGTGGCATTCTGGTGTTACCTGTCGGTGAGCAGGCCCAAACTCTGAAGTACATTCGGCGACAGGGAAG
CGAGTTTGTTATCGATACCGTCGAAGCGGTGCGTTTTGTTCCCCTGGTGAAGGGTGAACTGGCCTAG

Upstream 100 bases:

>100_bases
AGGGTTATGTGGCCATCACGCCACTGCAGGTGGATTTAACCGCTTATGCAGCCCAGGACGTGGTAAAAACGTGGTTAACC
AAAGCAGGGGTTGGCGGGGA

Downstream 100 bases:

>100_bases
CGATTGTCTGAATTTTCGGCAGGAAACGAAATCTGTTGCACTTTCATCACCTCCGGTTAATGTTTTAACGTTTCAATAAA
ACTAATTTTCAATACAATTG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MVNKRMQTLLTQLRQQGIRDERLLQAIEAVPRERFVDEALEHKAYENTALPIGSGQTISQPYMVARMTELLNLTPTSRVL
EIGTGSGYQTAILAHLVQHVCSVERIKGLQWQAKRRLKQLDLHNVSTRHGDGWQGWASRGPFDAIIVTAAPPEIPPALME
QLDDGGILVLPVGEQAQTLKYIRRQGSEFVIDTVEAVRFVPLVKGELA

Sequences:

>Translated_208_residues
MVNKRMQTLLTQLRQQGIRDERLLQAIEAVPRERFVDEALEHKAYENTALPIGSGQTISQPYMVARMTELLNLTPTSRVL
EIGTGSGYQTAILAHLVQHVCSVERIKGLQWQAKRRLKQLDLHNVSTRHGDGWQGWASRGPFDAIIVTAAPPEIPPALME
QLDDGGILVLPVGEQAQTLKYIRRQGSEFVIDTVEAVRFVPLVKGELA
>Mature_208_residues
MVNKRMQTLLTQLRQQGIRDERLLQAIEAVPRERFVDEALEHKAYENTALPIGSGQTISQPYMVARMTELLNLTPTSRVL
EIGTGSGYQTAILAHLVQHVCSVERIKGLQWQAKRRLKQLDLHNVSTRHGDGWQGWASRGPFDAIIVTAAPPEIPPALME
QLDDGGILVLPVGEQAQTLKYIRRQGSEFVIDTVEAVRFVPLVKGELA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=212, Percent_Identity=32.5471698113208, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1789100, Length=208, Percent_Identity=82.2115384615385, Blast_Score=355, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71983477, Length=212, Percent_Identity=32.0754716981132, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=211, Percent_Identity=31.2796208530806, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17981723, Length=221, Percent_Identity=31.6742081447964, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_SERP5 (A8G9Z6)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477064.1
- ProteinModelPortal:   A8G9Z6
- SMR:   A8G9Z6
- STRING:   A8G9Z6
- GeneID:   5602884
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0830
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   YMVARMS
- ProtClustDB:   PRK00312
- BioCyc:   SPRO399741:SPRO_0830-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23224; Mature: 23224

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 59-59

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNKRMQTLLTQLRQQGIRDERLLQAIEAVPRERFVDEALEHKAYENTALPIGSGQTISQ
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PYMVARMTELLNLTPTSRVLEIGTGSGYQTAILAHLVQHVCSVERIKGLQWQAKRRLKQL
HHHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DLHNVSTRHGDGWQGWASRGPFDAIIVTAAPPEIPPALMEQLDDGGILVLPVGEQAQTLK
HHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEECCCHHHHHH
YIRRQGSEFVIDTVEAVRFVPLVKGELA
HHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVNKRMQTLLTQLRQQGIRDERLLQAIEAVPRERFVDEALEHKAYENTALPIGSGQTISQ
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PYMVARMTELLNLTPTSRVLEIGTGSGYQTAILAHLVQHVCSVERIKGLQWQAKRRLKQL
HHHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DLHNVSTRHGDGWQGWASRGPFDAIIVTAAPPEIPPALMEQLDDGGILVLPVGEQAQTLK
HHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEECCCHHHHHH
YIRRQGSEFVIDTVEAVRFVPLVKGELA
HHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA