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The map label for this gene is hisJ [H]

Identifier: 157369069

GI number: 157369069

Start: 918680

End: 919450

Strand: Reverse

Name: hisJ [H]

Synonym: Spro_0824

Alternate gene names: 157369069

Gene position: 919450-918680 (Counterclockwise)

Preceding gene: 157369078

Following gene: 157369068

Centisome position: 16.87

GC content: 53.44

Gene sequence:

>771_bases
ATGTTAAAACGCATAATTCTGATTGGCGCCTGCCTGGCCGCCACCCTGTCGGCTTCTGCTCTGGCGGCAGAACCCACTTA
CGTCGTCGGCTCCGGCGGCACCTATCGTCCGTTCGAATTCGAAAACAGCAGCAAACAGCTGGAAGGCTTTGATATTGATA
TCATCAAGGCCGTCGCCAAGGCCGAAGGTTTCCAGGTCAAGCTGGTAAATACACCGTGGGAAGGCATCTTTGCCACGCTG
AACTCCGGCGATCGCGACATCATTATTTCCGGCATCACCATCACCGATAAACGCAAGCAAATGGTTGATTTCTCTGCGCC
TTACTTCCCGGCGGAGCAGACCATCGTGGTTCCCAAAGACTCTCAAGTGAACTCTATTGCCGCGCTGAAAGCGCTGAAAG
TTGGCGTGGTGAACTCCAGCACCGGCGACATCGTGGTATCCGACGTGCTGGGTAAAAACAGCACCGCCATCAAACGTTTC
GACAATACCCCGCTGCTGCTGCAGGAGTTGTACGAAGACGGCATCGGCGCAGCCGTAGGTGACGTTGGCGTAGCCAAGTT
CTACATAAAAACCCACCCGGAAAAAGAGTTCAAATTGGTGTCAGACGCCAAATTCGAACGTCAGTATTTCGGCATTGCCG
TTGCCAAAGGCAACGATGAACTACGCGGTAAAATCAACGCCGGCCTGAAGAAAATCATCGCCGACGGCACCTACGCCAAA
ATTTACCAGACCTGGTTTGACAGCAACGTGCCGACACTGCCTGCCGAATAA

Upstream 100 bases:

>100_bases
ATCAGGTAGGAGTTGTATTTTTATTCATTGAAGGTTATTTTTATGCACACATAGTGCATAAATAAATCCAATGAATAACT
TTTAATTATCGGGGATAGTG

Downstream 100 bases:

>100_bases
GCCTTAAGTGACCGGAGCTCGCACGAGCTCCGGAGCAACACTATCGTGAGAACACCGAGAAGGAACACCCATTGAATTTC
CGTTGGGAGATTATCCAGGA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLKRIILIGACLAATLSASALAAEPTYVVGSGGTYRPFEFENSSKQLEGFDIDIIKAVAKAEGFQVKLVNTPWEGIFATL
NSGDRDIIISGITITDKRKQMVDFSAPYFPAEQTIVVPKDSQVNSIAALKALKVGVVNSSTGDIVVSDVLGKNSTAIKRF
DNTPLLLQELYEDGIGAAVGDVGVAKFYIKTHPEKEFKLVSDAKFERQYFGIAVAKGNDELRGKINAGLKKIIADGTYAK
IYQTWFDSNVPTLPAE

Sequences:

>Translated_256_residues
MLKRIILIGACLAATLSASALAAEPTYVVGSGGTYRPFEFENSSKQLEGFDIDIIKAVAKAEGFQVKLVNTPWEGIFATL
NSGDRDIIISGITITDKRKQMVDFSAPYFPAEQTIVVPKDSQVNSIAALKALKVGVVNSSTGDIVVSDVLGKNSTAIKRF
DNTPLLLQELYEDGIGAAVGDVGVAKFYIKTHPEKEFKLVSDAKFERQYFGIAVAKGNDELRGKINAGLKKIIADGTYAK
IYQTWFDSNVPTLPAE
>Mature_256_residues
MLKRIILIGACLAATLSASALAAEPTYVVGSGGTYRPFEFENSSKQLEGFDIDIIKAVAKAEGFQVKLVNTPWEGIFATL
NSGDRDIIISGITITDKRKQMVDFSAPYFPAEQTIVVPKDSQVNSIAALKALKVGVVNSSTGDIVVSDVLGKNSTAIKRF
DNTPLLLQELYEDGIGAAVGDVGVAKFYIKTHPEKEFKLVSDAKFERQYFGIAVAKGNDELRGKINAGLKKIIADGTYAK
IYQTWFDSNVPTLPAE

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=241, Percent_Identity=34.4398340248963, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=31.6, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1787085, Length=249, Percent_Identity=30.9236947791165, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788228, Length=264, Percent_Identity=29.9242424242424, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=28.9473684210526, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=27.6264591439689, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27673; Mature: 27673

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRIILIGACLAATLSASALAAEPTYVVGSGGTYRPFEFENSSKQLEGFDIDIIKAVAK
CCCHHEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHH
AEGFQVKLVNTPWEGIFATLNSGDRDIIISGITITDKRKQMVDFSAPYFPAEQTIVVPKD
CCCCEEEEECCCCCCEEEEECCCCCEEEEEEEEEECCHHHHEECCCCCCCCCCEEEECCC
SQVNSIAALKALKVGVVNSSTGDIVVSDVLGKNSTAIKRFDNTPLLLQELYEDGIGAAVG
CCCHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHCCCCCHHC
DVGVAKFYIKTHPEKEFKLVSDAKFERQYFGIAVAKGNDELRGKINAGLKKIIADGTYAK
CCCEEEEEEEECCCHHEEEECCCHHCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHH
IYQTWFDSNVPTLPAE
HHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLKRIILIGACLAATLSASALAAEPTYVVGSGGTYRPFEFENSSKQLEGFDIDIIKAVAK
CCCHHEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHH
AEGFQVKLVNTPWEGIFATLNSGDRDIIISGITITDKRKQMVDFSAPYFPAEQTIVVPKD
CCCCEEEEECCCCCCEEEEECCCCCEEEEEEEEEECCHHHHEECCCCCCCCCCEEEECCC
SQVNSIAALKALKVGVVNSSTGDIVVSDVLGKNSTAIKRFDNTPLLLQELYEDGIGAAVG
CCCHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHCCCCCHHC
DVGVAKFYIKTHPEKEFKLVSDAKFERQYFGIAVAKGNDELRGKINAGLKKIIADGTYAK
CCCEEEEEEEECCCHHEEEECCCHHCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHH
IYQTWFDSNVPTLPAE
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]