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The map label for this gene is cysJ

Identifier: 157369055

GI number: 157369055

Start: 905347

End: 907146

Strand: Direct

Name: cysJ

Synonym: Spro_0810

Alternate gene names: 157369055

Gene position: 905347-907146 (Clockwise)

Preceding gene: 157369053

Following gene: 157369056

Centisome position: 16.62

GC content: 58.89

Gene sequence:

>1800_bases
ATGACGACTCAGGCACCTCCAACATCTTTGCTCCCGCTGACGCCCGAACAGCTGGCGCGCCTGCAGGCGGCGATTGGTGA
ATATTCACCAACGCAGCTGGCGTGGCTGTCAGGTTATTTCTGGGGAATGGTTAATCAACAGCCTGGCAGCGTTGCCATTG
CGCCGGTGGCTTCCGCTGCCGCCAGCATCACGGTGATCTCTGCTTCACAGACTGGCAATGCACGTCGCCTGGCGGAACAG
CTGCGCGACGATCTGTTGGCCGCCAAGCTGAGCGTCACCCTGGTGAATGCCGGTGACTACAAGTTCAAGCAGATTGCCCA
GGAACGCTTGCTGGTGATCGTTGCCTCCACACAGGGGGAAGGCGAGCCGGCGGAAGAGGCGGTTGCGCTGCATAAATTCC
TGTTCTCGAAGAAGGCACCCAAGCTTAACGAAACCGCGTTTGCGGTCTTCGGGCTGGGGGATACTTCCTACGAAAACTTC
TGTCAGTCGGGCAAAGACTTCGACGGCAAGCTGGCCGAACTGGGTGCCGAGCGCCTGGTCGAGCGGGTAGATGCCGACGT
GGAATATCAGGAACTGGCCACGGCCTGGCGTAAACAGGTAGTGGAAGTGCTGAAAGCCCGTGCGCCGGCGGAAAATGCCG
CACCGGGCGTGTTGGCCAGCGGCGCGGTTGACCTGCTCGATAGCAGCCCATACAGCAAAGAACAGCCGCTGACCGCGCAA
CTGGCGGTGAAGCAAAAAATTACCGGCCGTGCGTCAGATAAAGACGTGCGCCATATCGAGATTGATCTGGGCGATTCGGG
TCTGCGCTACCGACCGGGTGACGCGCTGGGCGTCTGGTTCGATAACGATCCTGCGCTGGTGGACGAACTGGTGCAACTGC
TGTGGTTGAAAGGCGACGAGCCGGTAGAGGTCGAAGGTAAAACTCTGCCGCTGGCGCAAGCGTTGCGCAGCCATTTCGAA
TTGACGCAGAACACTACGCCGATCGTCGACAAATACGCGGCGCTGTCGCGCGACGAGAAATTGATTGGCCTACTGGCAGA
TAAAGCCGCGCTGCAGCATTACGCGCACAACACGCCGATTGTCGACATGGTGCGTCAGGCACCGGCCGATCTCAATGCCG
AACAGTTGATTGGCCTGTTGCGCCCTCTGACGCCGCGCCTGTATTCCATCGCTTCGTCGCAGGCTGAGAACGAAAACGAA
GTGCACGTTACCGTCGGCGTGGTGCGCTACGACATTGACGGTCGCGCTCGTGCCGGTGGCGCTTCCAGCTTCCTGGCCGA
TCGCCTGGAAGAGGACGGTGATGTGCGGGTGTTCATCGAGCACAACGACAACTTCCGCCTGCCGGCCAACCCGGAAACGC
CGGTGATCATGATTGGCCCTGGCACCGGCATTGCGCCGTTCCGCGCCTTTATGCAGCAACGTGACGCTGATGGCGCCGGC
GGTAAGAATTGGCTGTTCTTCGGCAACCCGCACTTTACCGAAGATTTCCTGTATCAGGTCGAATGGCAGCGCTATGTGAA
GGATGGCCTGCTGACTCGTATCGATCTGGCCTGGTCACGCGATCAACAACATAAAGTCTATGTTCAAGACAAACTGCGCG
AACAGGGCGCGGAAGTGTGGCGTTGGATCCAGGAAGGCGCGCATATTTACGTCTGTGGCGATGCCAACCGCATGGCGAAG
GACGTGGAAAACACATTACTGGAACTGGTGGCCGAGCACGGTGGTATGGATACCGAGCTGGCGGATGAATTTTTAAGTGA
GCTGCGCCTTGAGCGCCGTTATCAGCGAGATGTTTACTGA

Upstream 100 bases:

>100_bases
TCCTGATCTTTTCAGGACCGCAGACCGAACGAAAAAATAGCGGCTGACACTGGCGAGCACCTGGCTGGATAGGTGCCATG
TATAGGAAATAAGTACTGCA

Downstream 100 bases:

>100_bases
TGAGTGATAAACACCCGGGGCCTCTGGTGGTTGAAGGCAAATTGGCCGATGCCGAGCGTTTGAAGAAAGAAAGCAACTTC
CTGCGCGGCACCATCGCTGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTTQAPPTSLLPLTPEQLARLQAAIGEYSPTQLAWLSGYFWGMVNQQPGSVAIAPVASAAASITVISASQTGNARRLAEQ
LRDDLLAAKLSVTLVNAGDYKFKQIAQERLLVIVASTQGEGEPAEEAVALHKFLFSKKAPKLNETAFAVFGLGDTSYENF
CQSGKDFDGKLAELGAERLVERVDADVEYQELATAWRKQVVEVLKARAPAENAAPGVLASGAVDLLDSSPYSKEQPLTAQ
LAVKQKITGRASDKDVRHIEIDLGDSGLRYRPGDALGVWFDNDPALVDELVQLLWLKGDEPVEVEGKTLPLAQALRSHFE
LTQNTTPIVDKYAALSRDEKLIGLLADKAALQHYAHNTPIVDMVRQAPADLNAEQLIGLLRPLTPRLYSIASSQAENENE
VHVTVGVVRYDIDGRARAGGASSFLADRLEEDGDVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRDADGAG
GKNWLFFGNPHFTEDFLYQVEWQRYVKDGLLTRIDLAWSRDQQHKVYVQDKLREQGAEVWRWIQEGAHIYVCGDANRMAK
DVENTLLELVAEHGGMDTELADEFLSELRLERRYQRDVY

Sequences:

>Translated_599_residues
MTTQAPPTSLLPLTPEQLARLQAAIGEYSPTQLAWLSGYFWGMVNQQPGSVAIAPVASAAASITVISASQTGNARRLAEQ
LRDDLLAAKLSVTLVNAGDYKFKQIAQERLLVIVASTQGEGEPAEEAVALHKFLFSKKAPKLNETAFAVFGLGDTSYENF
CQSGKDFDGKLAELGAERLVERVDADVEYQELATAWRKQVVEVLKARAPAENAAPGVLASGAVDLLDSSPYSKEQPLTAQ
LAVKQKITGRASDKDVRHIEIDLGDSGLRYRPGDALGVWFDNDPALVDELVQLLWLKGDEPVEVEGKTLPLAQALRSHFE
LTQNTTPIVDKYAALSRDEKLIGLLADKAALQHYAHNTPIVDMVRQAPADLNAEQLIGLLRPLTPRLYSIASSQAENENE
VHVTVGVVRYDIDGRARAGGASSFLADRLEEDGDVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRDADGAG
GKNWLFFGNPHFTEDFLYQVEWQRYVKDGLLTRIDLAWSRDQQHKVYVQDKLREQGAEVWRWIQEGAHIYVCGDANRMAK
DVENTLLELVAEHGGMDTELADEFLSELRLERRYQRDVY
>Mature_598_residues
TTQAPPTSLLPLTPEQLARLQAAIGEYSPTQLAWLSGYFWGMVNQQPGSVAIAPVASAAASITVISASQTGNARRLAEQL
RDDLLAAKLSVTLVNAGDYKFKQIAQERLLVIVASTQGEGEPAEEAVALHKFLFSKKAPKLNETAFAVFGLGDTSYENFC
QSGKDFDGKLAELGAERLVERVDADVEYQELATAWRKQVVEVLKARAPAENAAPGVLASGAVDLLDSSPYSKEQPLTAQL
AVKQKITGRASDKDVRHIEIDLGDSGLRYRPGDALGVWFDNDPALVDELVQLLWLKGDEPVEVEGKTLPLAQALRSHFEL
TQNTTPIVDKYAALSRDEKLIGLLADKAALQHYAHNTPIVDMVRQAPADLNAEQLIGLLRPLTPRLYSIASSQAENENEV
HVTVGVVRYDIDGRARAGGASSFLADRLEEDGDVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRDADGAGG
KNWLFFGNPHFTEDFLYQVEWQRYVKDGLLTRIDLAWSRDQQHKVYVQDKLREQGAEVWRWIQEGAHIYVCGDANRMAKD
VENTLLELVAEHGGMDTELADEFLSELRLERRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain

Homologues:

Organism=Homo sapiens, GI127139033, Length=613, Percent_Identity=30.8319738988581, Blast_Score=253, Evalue=4e-67,
Organism=Homo sapiens, GI24041029, Length=596, Percent_Identity=32.2147651006711, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI7657393, Length=597, Percent_Identity=30.6532663316583, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI221316709, Length=597, Percent_Identity=30.6532663316583, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI221316705, Length=606, Percent_Identity=30.1980198019802, Blast_Score=217, Evalue=3e-56,
Organism=Homo sapiens, GI10835173, Length=645, Percent_Identity=28.6821705426357, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI221316707, Length=531, Percent_Identity=30.8851224105461, Blast_Score=196, Evalue=7e-50,
Organism=Homo sapiens, GI40254422, Length=656, Percent_Identity=28.0487804878049, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI169790956, Length=437, Percent_Identity=28.8329519450801, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI169790958, Length=437, Percent_Identity=28.8329519450801, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1789123, Length=601, Percent_Identity=78.5357737104825, Blast_Score=960, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=636, Percent_Identity=30.0314465408805, Blast_Score=257, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17566446, Length=576, Percent_Identity=24.6527777777778, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17531441, Length=397, Percent_Identity=27.455919395466, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321832, Length=632, Percent_Identity=30.2215189873418, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6321143, Length=412, Percent_Identity=29.6116504854369, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6325305, Length=635, Percent_Identity=23.7795275590551, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137192, Length=609, Percent_Identity=29.3924466338259, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24582192, Length=510, Percent_Identity=30.7843137254902, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI78706872, Length=524, Percent_Identity=32.824427480916, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24583543, Length=524, Percent_Identity=32.6335877862595, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI78706876, Length=524, Percent_Identity=32.6335877862595, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24660907, Length=598, Percent_Identity=26.9230769230769, Blast_Score=149, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24660903, Length=598, Percent_Identity=26.9230769230769, Blast_Score=149, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24647438, Length=427, Percent_Identity=22.9508196721311, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSJ_SERP5 (A8G9X6)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477044.1
- ProteinModelPortal:   A8G9X6
- SMR:   A8G9X6
- STRING:   A8G9X6
- GeneID:   5603583
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0810
- eggNOG:   COG0369
- HOGENOM:   HBG736048
- OMA:   HVEIDLG
- ProtClustDB:   PRK10953
- BioCyc:   SPRO399741:SPRO_0810-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01541
- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.20.990.10
- PIRSF:   PIRSF000207
- PRINTS:   PR00369
- PRINTS:   PR00371
- TIGRFAMs:   TIGR01931

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.8.1.2

Molecular weight: Translated: 66202; Mature: 66071

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS51384 FAD_FR; PS50902 FLAVODOXIN_LIKE

Important sites: BINDING 489-489

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQAPPTSLLPLTPEQLARLQAAIGEYSPTQLAWLSGYFWGMVNQQPGSVAIAPVASAA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEHHHCC
ASITVISASQTGNARRLAEQLRDDLLAAKLSVTLVNAGDYKFKQIAQERLLVIVASTQGE
EEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHCCEEEEEEECCCC
GEPAEEAVALHKFLFSKKAPKLNETAFAVFGLGDTSYENFCQSGKDFDGKLAELGAERLV
CCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHH
ERVDADVEYQELATAWRKQVVEVLKARAPAENAAPGVLASGAVDLLDSSPYSKEQPLTAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHH
LAVKQKITGRASDKDVRHIEIDLGDSGLRYRPGDALGVWFDNDPALVDELVQLLWLKGDE
HHHHHHHCCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVEVEGKTLPLAQALRSHFELTQNTTPIVDKYAALSRDEKLIGLLADKAALQHYAHNTPI
CEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCH
VDMVRQAPADLNAEQLIGLLRPLTPRLYSIASSQAENENEVHVTVGVVRYDIDGRARAGG
HHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRLEEDGDVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRDADGAG
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKDGLLTRIDLAWSRDQQHKVYVQDKLREQGAEVW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHH
RWIQEGAHIYVCGDANRMAKDVENTLLELVAEHGGMDTELADEFLSELRLERRYQRDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQAPPTSLLPLTPEQLARLQAAIGEYSPTQLAWLSGYFWGMVNQQPGSVAIAPVASAA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEHHHCC
ASITVISASQTGNARRLAEQLRDDLLAAKLSVTLVNAGDYKFKQIAQERLLVIVASTQGE
EEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHCCEEEEEEECCCC
GEPAEEAVALHKFLFSKKAPKLNETAFAVFGLGDTSYENFCQSGKDFDGKLAELGAERLV
CCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHH
ERVDADVEYQELATAWRKQVVEVLKARAPAENAAPGVLASGAVDLLDSSPYSKEQPLTAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHH
LAVKQKITGRASDKDVRHIEIDLGDSGLRYRPGDALGVWFDNDPALVDELVQLLWLKGDE
HHHHHHHCCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVEVEGKTLPLAQALRSHFELTQNTTPIVDKYAALSRDEKLIGLLADKAALQHYAHNTPI
CEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCH
VDMVRQAPADLNAEQLIGLLRPLTPRLYSIASSQAENENEVHVTVGVVRYDIDGRARAGG
HHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRLEEDGDVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRDADGAG
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKDGLLTRIDLAWSRDQQHKVYVQDKLREQGAEVW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHH
RWIQEGAHIYVCGDANRMAKDVENTLLELVAEHGGMDTELADEFLSELRLERRYQRDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA