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The map label for this gene is cysI

Identifier: 157369056

GI number: 157369056

Start: 907146

End: 908861

Strand: Direct

Name: cysI

Synonym: Spro_0811

Alternate gene names: 157369056

Gene position: 907146-908861 (Clockwise)

Preceding gene: 157369055

Following gene: 157369057

Centisome position: 16.65

GC content: 58.51

Gene sequence:

>1716_bases
ATGAGTGATAAACACCCGGGGCCTCTGGTGGTTGAAGGCAAATTGGCCGATGCCGAGCGTTTGAAGAAAGAAAGCAACTT
CCTGCGCGGCACCATCGCTGAAGATTTGAAGGACGGTTTGACCGGTGGTTTTACCGGTGACAACTTCCTGCTGATCCGTT
TTCACGGCATGTACCAGCAGGACGACCGTGATATTCGCGCCGAACGCGCCGAGCAGAAGCTGGAGCCGCGCCACGCGATG
ATGCTGCGCTGCCGTCTGCCGGGGGGGATTATCTCACCCCAACAGTGGCTGGGCATTGATAAGTTCGCGCAGGAAAGCAC
CCTGTACGGCAGCATTCGTATCACCAACCGCCAGACCTTCCAGTTCCATGGCATTTTGAAGGGCAACGTGAAGCCGGTTC
ACCAACTGCTGGGCCGCCTGGGGCTGGACGCGCTGGCTACCGCCAACGACGTTAACCGTAACGTGCTTTGTACTTCTAAC
CCGGTGGAGTCAGAACTGCATCAGGAAGCCTACGAGTGGGCGAAGAAGATTTCCGAACATCTGTTGCCGCGCACCCGTGC
TTACGCGGAAGTGTGGCTGGATCAGGAAAAAGTGGCGACCACCGACGAAGAACCGATCCTCGGGGCGACCTATCTGCCGC
GCAAGTTCAAAACCACGGTGGTGATCCCACCGCAGAATGACGTTGATCTGCACGCCAACGACATGAACTTTGTGGCGATC
GCCAAAAACGGCAAGCTGGTGGGCTTCAACCTGCTGGTGGGCGGCGGTCTGTCGATTGAACATGGCAACAAGAAAACTTA
TGCGCGCCAGGCCAGCGAGTTCGGCTACATCCCGCTGGAGCACACGCTGGCAGTCGCCGAAGCGGTAGTGACCACCCAGC
GCGACTGGGGTAATCGTACCGATCGTAAAAATGCCAAAACCAAATACACGCTGGAGCGCGTCGGGGTGGATACTTTCCGC
GCCGAAGTAGAAAAACGGGCAGGCATCACTTTTGAGCCGATCCGCCCGTACGAATTCACCGGTCGCGGCGATCGCATTGG
TTGGGTGAAAGGTATTGATAACCAATGGCACCTGACGCTGTTTATTGAGAACGGCCGGTTATTGGATTACCCGGGGCGTC
CGCTGAAAACCGGCGTTGCCGAGATCGCGCGGATCCACAAAGGGGATTTCCGCCTGACGGCCAACCAGAACCTGATCGTC
GCCGGTGTGCCGGAAAGCGAGAAGGCGAAGATCGAAGCTCTGGCGCGAGATCATGGGCTGATCGACGATGGGGTCAGCGT
ACAGCGCCAAAACTCGATGGCCTGCGTATCCTTCCCGACCTGCCCGTTGGCAATGGCGGAGGCCGAGCGTTTCTTGCCGG
AGTTCGTCACCAAGGTGGAAGGGATCCTGCATCGGCACGGCGTGGGGGATGAGCACATCGTATTGCGCATCACCGGCTGC
CCGAATGGCTGTGGCCGCGCACTGCTGGCGGAGATTGGCCTGGTGGGTAAAGCGGTAGGTCGCTATAACCTGCATTTGGG
CGGTAACCGGGAAGGCACGCGTATTCCGCGCATGTACCGAGAAAATATTAACGAAGAAGAAATTCTGCGAGAGATTGACC
AACTGGTGGGCCGTTGGGCCACCGAGCGCACGGCAGGCGAAGGCTTTGGTGATTTCACCATCCGCGCCGGTGTGGTCAGG
CCGGTGGTCGATCCGGCGCAGGATTTCTGGGACTGA

Upstream 100 bases:

>100_bases
TGGAACTGGTGGCCGAGCACGGTGGTATGGATACCGAGCTGGCGGATGAATTTTTAAGTGAGCTGCGCCTTGAGCGCCGT
TATCAGCGAGATGTTTACTG

Downstream 100 bases:

>100_bases
CGCCATTTGGCCCCGCTGGTACAGGGGGCTGGATCTGCGCGGTATGATTCAAGCATCAGGAGGCAGGCAACATGGCTGAA
TTCAATCTGGCGGAGCTGAA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MSDKHPGPLVVEGKLADAERLKKESNFLRGTIAEDLKDGLTGGFTGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAM
MLRCRLPGGIISPQQWLGIDKFAQESTLYGSIRITNRQTFQFHGILKGNVKPVHQLLGRLGLDALATANDVNRNVLCTSN
PVESELHQEAYEWAKKISEHLLPRTRAYAEVWLDQEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDMNFVAI
AKNGKLVGFNLLVGGGLSIEHGNKKTYARQASEFGYIPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVDTFR
AEVEKRAGITFEPIRPYEFTGRGDRIGWVKGIDNQWHLTLFIENGRLLDYPGRPLKTGVAEIARIHKGDFRLTANQNLIV
AGVPESEKAKIEALARDHGLIDDGVSVQRQNSMACVSFPTCPLAMAEAERFLPEFVTKVEGILHRHGVGDEHIVLRITGC
PNGCGRALLAEIGLVGKAVGRYNLHLGGNREGTRIPRMYRENINEEEILREIDQLVGRWATERTAGEGFGDFTIRAGVVR
PVVDPAQDFWD

Sequences:

>Translated_571_residues
MSDKHPGPLVVEGKLADAERLKKESNFLRGTIAEDLKDGLTGGFTGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAM
MLRCRLPGGIISPQQWLGIDKFAQESTLYGSIRITNRQTFQFHGILKGNVKPVHQLLGRLGLDALATANDVNRNVLCTSN
PVESELHQEAYEWAKKISEHLLPRTRAYAEVWLDQEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDMNFVAI
AKNGKLVGFNLLVGGGLSIEHGNKKTYARQASEFGYIPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVDTFR
AEVEKRAGITFEPIRPYEFTGRGDRIGWVKGIDNQWHLTLFIENGRLLDYPGRPLKTGVAEIARIHKGDFRLTANQNLIV
AGVPESEKAKIEALARDHGLIDDGVSVQRQNSMACVSFPTCPLAMAEAERFLPEFVTKVEGILHRHGVGDEHIVLRITGC
PNGCGRALLAEIGLVGKAVGRYNLHLGGNREGTRIPRMYRENINEEEILREIDQLVGRWATERTAGEGFGDFTIRAGVVR
PVVDPAQDFWD
>Mature_570_residues
SDKHPGPLVVEGKLADAERLKKESNFLRGTIAEDLKDGLTGGFTGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMM
LRCRLPGGIISPQQWLGIDKFAQESTLYGSIRITNRQTFQFHGILKGNVKPVHQLLGRLGLDALATANDVNRNVLCTSNP
VESELHQEAYEWAKKISEHLLPRTRAYAEVWLDQEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDMNFVAIA
KNGKLVGFNLLVGGGLSIEHGNKKTYARQASEFGYIPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVDTFRA
EVEKRAGITFEPIRPYEFTGRGDRIGWVKGIDNQWHLTLFIENGRLLDYPGRPLKTGVAEIARIHKGDFRLTANQNLIVA
GVPESEKAKIEALARDHGLIDDGVSVQRQNSMACVSFPTCPLAMAEAERFLPEFVTKVEGILHRHGVGDEHIVLRITGCP
NGCGRALLAEIGLVGKAVGRYNLHLGGNREGTRIPRMYRENINEEEILREIDQLVGRWATERTAGEGFGDFTIRAGVVRP
VVDPAQDFWD

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=571, Percent_Identity=83.8879159369527, Blast_Score=1028, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=587, Percent_Identity=43.4412265758092, Blast_Score=500, Evalue=1e-142,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_SERP5 (A8G9X7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477045.1
- ProteinModelPortal:   A8G9X7
- SMR:   A8G9X7
- STRING:   A8G9X7
- GeneID:   5603584
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0811
- eggNOG:   COG0155
- HOGENOM:   HBG626671
- OMA:   VGDEHIV
- ProtClustDB:   PRK13504
- BioCyc:   SPRO399741:SPRO_0811-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 63874; Mature: 63742

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKHPGPLVVEGKLADAERLKKESNFLRGTIAEDLKDGLTGGFTGDNFLLIRFHGMYQQ
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
DDRDIRAERAEQKLEPRHAMMLRCRLPGGIISPQQWLGIDKFAQESTLYGSIRITNRQTF
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHCCEEEEEEEECCCEEE
QFHGILKGNVKPVHQLLGRLGLDALATANDVNRNVLCTSNPVESELHQEAYEWAKKISEH
EEEEEEECCCHHHHHHHHHHCCHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LLPRTRAYAEVWLDQEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDMNFVAI
HCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCEEEEE
AKNGKLVGFNLLVGGGLSIEHGNKKTYARQASEFGYIPLEHTLAVAEAVVTTQRDWGNRT
ECCCEEEEEEEEEECCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
DRKNAKTKYTLERVGVDTFRAEVEKRAGITFEPIRPYEFTGRGDRIGWVKGIDNQWHLTL
CCCCCCCEEEHHHHCHHHHHHHHHHHCCCEECCCCCEEECCCCCEEEEEECCCCCEEEEE
FIENGRLLDYPGRPLKTGVAEIARIHKGDFRLTANQNLIVAGVPESEKAKIEALARDHGL
EEECCEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
IDDGVSVQRQNSMACVSFPTCPLAMAEAERFLPEFVTKVEGILHRHGVGDEHIVLRITGC
CCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
PNGCGRALLAEIGLVGKAVGRYNLHLGGNREGTRIPRMYRENINEEEILREIDQLVGRWA
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
TERTAGEGFGDFTIRAGVVRPVVDPAQDFWD
HHCCCCCCCCCEEEECCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SDKHPGPLVVEGKLADAERLKKESNFLRGTIAEDLKDGLTGGFTGDNFLLIRFHGMYQQ
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
DDRDIRAERAEQKLEPRHAMMLRCRLPGGIISPQQWLGIDKFAQESTLYGSIRITNRQTF
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHCCEEEEEEEECCCEEE
QFHGILKGNVKPVHQLLGRLGLDALATANDVNRNVLCTSNPVESELHQEAYEWAKKISEH
EEEEEEECCCHHHHHHHHHHCCHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LLPRTRAYAEVWLDQEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDMNFVAI
HCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCEEEEE
AKNGKLVGFNLLVGGGLSIEHGNKKTYARQASEFGYIPLEHTLAVAEAVVTTQRDWGNRT
ECCCEEEEEEEEEECCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
DRKNAKTKYTLERVGVDTFRAEVEKRAGITFEPIRPYEFTGRGDRIGWVKGIDNQWHLTL
CCCCCCCEEEHHHHCHHHHHHHHHHHCCCEECCCCCEEECCCCCEEEEEECCCCCEEEEE
FIENGRLLDYPGRPLKTGVAEIARIHKGDFRLTANQNLIVAGVPESEKAKIEALARDHGL
EEECCEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
IDDGVSVQRQNSMACVSFPTCPLAMAEAERFLPEFVTKVEGILHRHGVGDEHIVLRITGC
CCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
PNGCGRALLAEIGLVGKAVGRYNLHLGGNREGTRIPRMYRENINEEEILREIDQLVGRWA
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
TERTAGEGFGDFTIRAGVVRPVVDPAQDFWD
HHCCCCCCCCCEEEECCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA