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The map label for this gene is ampE [H]

Identifier: 157369025

GI number: 157369025

Start: 869452

End: 870306

Strand: Direct

Name: ampE [H]

Synonym: Spro_0780

Alternate gene names: 157369025

Gene position: 869452-870306 (Clockwise)

Preceding gene: 157369020

Following gene: 157369027

Centisome position: 15.96

GC content: 58.25

Gene sequence:

>855_bases
ATGACGCTGTTTACGCTGTTACTGGTTCTGGCGTGGGAACGTTTGTTCAAGCTGGGTGAGCATTGGCAGTTGGACCATCG
GCTGGAGGTGGTGTTTCATCGGCTGCATCGGTTCTCGCTGGCGCAGACGTTGCTGATGACCCTGGCCTGGATGGTGGTGG
TCGGGGGCATTCTGTGGCTGTCTCAAGGGCTGTTTTTCGGCGTAGTGACGCTGTTGCTGTGGATTATCATTGATTTGCTG
TGTGTGGGTGCGGGCATCAAGCGTAAACACTATCGCGCCTATCTGAAAGCGGCGCGGCAGGGAGATGCTCATGCCAGCGA
CCAGATGGCGGAGGAGCTGGCACTGATCCACGGTTTGCCGGTGGATTGCAGCGAAGAGCTGCGTCTGCGTGAACTGCAAA
ATGCGTTGCTGTGGATTAACTTCCGTTATTATCTGGCACCGCTGTTCTGGTTCGTGGTCTGCGGGCCTTATGGGCCGATT
GCGCTGGCGGGCTACTCCTTCCTGCGTGCTTACCAGACCTGGTTGGCGCGCCATAATACCCCGCTGGAGCGTTCGCAGTC
GGGCATCGATCATCTGTTGAACTGGCTGGACTGGATCCCGGTGCGTCTGGCCGGTGTGGCTTACGCCCTGTTTGGACATG
GTGAGAGAGCACTGCCGGCATGGTTTGCATCGCTGGGCGATCTCCATTCTTCGCAGTATCAGGTGCTGACGCGGCTGGCA
CAGTTCTCACTGGCGCGTGATCCCCATATGGATCCGGTGCAGACCCCGCGGGCGGCGGTAACCCTGGCACGTAAGGTGAC
GATGATTATCGTGGTGGTGGTAGCCCTGCTGACGATTTACGGTACTTTGCTCTGA

Upstream 100 bases:

>100_bases
CGGCGATATTTTACCGGGATTTAAATCTGGCATCTCTGGAACAACCCGGCAAGCCCGATGACGCTATGCTTGGAACAATG
GAAAGGGAGAGGAGTCGCTA

Downstream 100 bases:

>100_bases
CGGCGAAATTTGGAAATAAAAACGGGCGCTGAGCGCCCGTTTTTATTGGATAGCCGAAACTTACAGTTTGGCGAAGCAGC
GGCGCGCGGCATCTATAGTG

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTLFTLLLVLAWERLFKLGEHWQLDHRLEVVFHRLHRFSLAQTLLMTLAWMVVVGGILWLSQGLFFGVVTLLLWIIIDLL
CVGAGIKRKHYRAYLKAARQGDAHASDQMAEELALIHGLPVDCSEELRLRELQNALLWINFRYYLAPLFWFVVCGPYGPI
ALAGYSFLRAYQTWLARHNTPLERSQSGIDHLLNWLDWIPVRLAGVAYALFGHGERALPAWFASLGDLHSSQYQVLTRLA
QFSLARDPHMDPVQTPRAAVTLARKVTMIIVVVVALLTIYGTLL

Sequences:

>Translated_284_residues
MTLFTLLLVLAWERLFKLGEHWQLDHRLEVVFHRLHRFSLAQTLLMTLAWMVVVGGILWLSQGLFFGVVTLLLWIIIDLL
CVGAGIKRKHYRAYLKAARQGDAHASDQMAEELALIHGLPVDCSEELRLRELQNALLWINFRYYLAPLFWFVVCGPYGPI
ALAGYSFLRAYQTWLARHNTPLERSQSGIDHLLNWLDWIPVRLAGVAYALFGHGERALPAWFASLGDLHSSQYQVLTRLA
QFSLARDPHMDPVQTPRAAVTLARKVTMIIVVVVALLTIYGTLL
>Mature_283_residues
TLFTLLLVLAWERLFKLGEHWQLDHRLEVVFHRLHRFSLAQTLLMTLAWMVVVGGILWLSQGLFFGVVTLLLWIIIDLLC
VGAGIKRKHYRAYLKAARQGDAHASDQMAEELALIHGLPVDCSEELRLRELQNALLWINFRYYLAPLFWFVVCGPYGPIA
LAGYSFLRAYQTWLARHNTPLERSQSGIDHLLNWLDWIPVRLAGVAYALFGHGERALPAWFASLGDLHSSQYQVLTRLAQ
FSLARDPHMDPVQTPRAAVTLARKVTMIIVVVVALLTIYGTLL

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=284, Percent_Identity=65.8450704225352, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 32380; Mature: 32248

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLFTLLLVLAWERLFKLGEHWQLDHRLEVVFHRLHRFSLAQTLLMTLAWMVVVGGILWL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQGLFFGVVTLLLWIIIDLLCVGAGIKRKHYRAYLKAARQGDAHASDQMAEELALIHGLP
HCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
VDCSEELRLRELQNALLWINFRYYLAPLFWFVVCGPYGPIALAGYSFLRAYQTWLARHNT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PLERSQSGIDHLLNWLDWIPVRLAGVAYALFGHGERALPAWFASLGDLHSSQYQVLTRLA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHH
QFSLARDPHMDPVQTPRAAVTLARKVTMIIVVVVALLTIYGTLL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]