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The map label for this gene is leuO [H]

Identifier: 157368991

GI number: 157368991

Start: 831312

End: 832259

Strand: Direct

Name: leuO [H]

Synonym: Spro_0746

Alternate gene names: 157368991

Gene position: 831312-832259 (Clockwise)

Preceding gene: 157368986

Following gene: 157368992

Centisome position: 15.26

GC content: 47.36

Gene sequence:

>948_bases
ATGTCGGAATGTAATTCAGGAGTTGTTATCGATAAAGAAACCAGCGAACTGCATTTGCGCAGCGTCGATCTGAATTTGCT
CACCGTGTTTGATGCCGTTATGCAGATGCAGAATATTACCCGGGCAGCGAGCTTTTTGGGGATGTCGCAGCCGGCGGTGA
GCAACGCCGTTGCACGTTTAAAGCTGATGTTTAATGACGAGCTGTTCGTCCGCTGTGGGCGTGGGATCCAGCCTACGGTG
CGGGCTAAGCAGCTGTTTGGCCCGGTTAGGCAGGCGTTGCAGTTGGTGCAAAATGAGTTGCCCGGAGCGGAGTTTGAACC
CTTTAGCAGTGAGCGAGCTTTCTCTATCGCCATTTGCAGTCCGCTTGATTTGCGTTTGGGGGCTACCATAATCAATCACA
TGAGGGAAATAGCGCCTCATACCCATTTGAAAATAAAGTCATATATAACCAATAACATCGATAACCAATTACGCTACCAG
GAGGTTGAGTTCGTTGTTGGCTATGCCAGCTTTGAGTCTTCAGAGTTTAAAAGTATGGCGCTTTTTGATGACGAGCTGGT
GCTGGTATTTTCCAGGAAACATCCGAGGATAGAAAAATCCATTACGCCGGAACAAATCCTGGCGGAGCAACACGCGGTGG
TTTCACTCGAGAGTTTTTATTCTTTCAGTAAACCCTACTATATAGAAGAAGCTATGCAACGAGTCGTGGATCAACAATGT
ACTGATTTATACAGCGTGCTGAATATAGTATCCAGTACTGACATGATTGCCATTGTTCCGGCCTGGCTGGCTCGACAGCA
GGCTGAATCCTTAAAGCTGCGTTTTATTCCTTTACCCTGGAATGAAAACAAGGTGACCTGTTATCTGTCCTGGCATGAGT
CGGCTGAACGGGATAAGGGACATCAATGGATGAAACTGATGCTTGGCCAGTCTGAGTTGACTGAATAA

Upstream 100 bases:

>100_bases
GAGAGATAACTAATTCTACCGTGTGAAATCAAGATGGCGCTGTTTTGTCATTTTTATTGCGCGTGGGGAATTATCTACAC
AGGACTTAGTGGAGTGGGAT

Downstream 100 bases:

>100_bases
ATCAGCCTATTATTACCCCCCCCGAGGTGGTGGTTTTCCATAGGGGGAATAATTTTCCCGTGCTTTGGTTAAGAATTATC
TTGGGTTTATCTAAGATATA

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSECNSGVVIDKETSELHLRSVDLNLLTVFDAVMQMQNITRAASFLGMSQPAVSNAVARLKLMFNDELFVRCGRGIQPTV
RAKQLFGPVRQALQLVQNELPGAEFEPFSSERAFSIAICSPLDLRLGATIINHMREIAPHTHLKIKSYITNNIDNQLRYQ
EVEFVVGYASFESSEFKSMALFDDELVLVFSRKHPRIEKSITPEQILAEQHAVVSLESFYSFSKPYYIEEAMQRVVDQQC
TDLYSVLNIVSSTDMIAIVPAWLARQQAESLKLRFIPLPWNENKVTCYLSWHESAERDKGHQWMKLMLGQSELTE

Sequences:

>Translated_315_residues
MSECNSGVVIDKETSELHLRSVDLNLLTVFDAVMQMQNITRAASFLGMSQPAVSNAVARLKLMFNDELFVRCGRGIQPTV
RAKQLFGPVRQALQLVQNELPGAEFEPFSSERAFSIAICSPLDLRLGATIINHMREIAPHTHLKIKSYITNNIDNQLRYQ
EVEFVVGYASFESSEFKSMALFDDELVLVFSRKHPRIEKSITPEQILAEQHAVVSLESFYSFSKPYYIEEAMQRVVDQQC
TDLYSVLNIVSSTDMIAIVPAWLARQQAESLKLRFIPLPWNENKVTCYLSWHESAERDKGHQWMKLMLGQSELTE
>Mature_314_residues
SECNSGVVIDKETSELHLRSVDLNLLTVFDAVMQMQNITRAASFLGMSQPAVSNAVARLKLMFNDELFVRCGRGIQPTVR
AKQLFGPVRQALQLVQNELPGAEFEPFSSERAFSIAICSPLDLRLGATIINHMREIAPHTHLKIKSYITNNIDNQLRYQE
VEFVVGYASFESSEFKSMALFDDELVLVFSRKHPRIEKSITPEQILAEQHAVVSLESFYSFSKPYYIEEAMQRVVDQQCT
DLYSVLNIVSSTDMIAIVPAWLARQQAESLKLRFIPLPWNENKVTCYLSWHESAERDKGHQWMKLMLGQSELTE

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=296, Percent_Identity=61.1486486486487, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1786820, Length=285, Percent_Identity=27.0175438596491, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081766, Length=285, Percent_Identity=24.9122807017544, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788887, Length=279, Percent_Identity=24.3727598566308, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35927; Mature: 35795

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSECNSGVVIDKETSELHLRSVDLNLLTVFDAVMQMQNITRAASFLGMSQPAVSNAVARL
CCCCCCCEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KLMFNDELFVRCGRGIQPTVRAKQLFGPVRQALQLVQNELPGAEFEPFSSERAFSIAICS
HHEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
PLDLRLGATIINHMREIAPHTHLKIKSYITNNIDNQLRYQEVEFVVGYASFESSEFKSMA
CCCHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCHHHHHHHEEEEEEEECCCCCCCCEEE
LFDDELVLVFSRKHPRIEKSITPEQILAEQHAVVSLESFYSFSKPYYIEEAMQRVVDQQC
EECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEHHHHHCCCCCHHHHHHHHHHHHHHH
TDLYSVLNIVSSTDMIAIVPAWLARQQAESLKLRFIPLPWNENKVTCYLSWHESAERDKG
HHHHHHHHHHCCCCHHEEHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCCCH
HQWMKLMLGQSELTE
HHHHHHHHCHHHCCC
>Mature Secondary Structure 
SECNSGVVIDKETSELHLRSVDLNLLTVFDAVMQMQNITRAASFLGMSQPAVSNAVARL
CCCCCCEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KLMFNDELFVRCGRGIQPTVRAKQLFGPVRQALQLVQNELPGAEFEPFSSERAFSIAICS
HHEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
PLDLRLGATIINHMREIAPHTHLKIKSYITNNIDNQLRYQEVEFVVGYASFESSEFKSMA
CCCHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCHHHHHHHEEEEEEEECCCCCCCCEEE
LFDDELVLVFSRKHPRIEKSITPEQILAEQHAVVSLESFYSFSKPYYIEEAMQRVVDQQC
EECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEHHHHHCCCCCHHHHHHHHHHHHHHH
TDLYSVLNIVSSTDMIAIVPAWLARQQAESLKLRFIPLPWNENKVTCYLSWHESAERDKG
HHHHHHHHHHCCCCHHEEHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCCCH
HQWMKLMLGQSELTE
HHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]