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The map label for this gene is yflS [H]

Identifier: 157368986

GI number: 157368986

Start: 824227

End: 825699

Strand: Direct

Name: yflS [H]

Synonym: Spro_0741

Alternate gene names: 157368986

Gene position: 824227-825699 (Clockwise)

Preceding gene: 157368985

Following gene: 157368991

Centisome position: 15.13

GC content: 57.98

Gene sequence:

>1473_bases
ATGGATAAACTAACGCCGCTCAAGCCCGTCCCTTCTCTGTGTGCCGTCGCCGCTGCTTTACTCATCTGGTTTGTGATCCC
GGTGCCTGAAGGCGTGGCACCCAATGCCTGGCATTTGCTGGCGCTGTTTATCGGCACCATCATCGCCATCATCGGCAAAG
CGATGCCGATTGGTGCCGTTTCGGTGGTGGCGATCGCGCTGGTGGCGGTAACCGGCGTCACCAATCCCGGCAAACCCGGT
GCTGCGCTCAATGACGCGCTCAGCGGATTTTCCAACCAGTTGATCTGGCTGATTGGTTTTTCGATCATGATCTCACTCAG
CCTGAACAAAACCGGGCTGGGGGCGCGCATCGGTTATTACTTCATCTCGCTGTTTGGTAAAAAGACGCTGGGCATTGCTT
ATGCATTGACGCTGGCGGAAACTACCCTGGCGCCGGTGACCCCAAGTAATACCGCGCGTGGCGGCGGGATTATCCACCCG
ATCATGAAGTCGATTGCCGACAGCTTCGGCTCCAAACCCGAGCTCAATACCTCCGGCAAGATTGGCCGCTATCTGTCGCT
GGTCAATTACAATATCAACCCGATCACTTCGGCGATGTTTATCACCGCTACCGCGCCGAACCCGTTGATCGTCAGCCTGA
TTGCCAAGGGCACTCATGGCAACTTTGAGCTGTCCTGGTCCATGTGGGCGGTGGCGGCACTGGTGCCTGGGCTGTGTTCG
CTGATTGTCATGCCCTTGGTGATTTATCTGCTCTACCCGCCAGAGGTGAAAAGCACCCCGGACGCGCCGCGTTTCGCTCG
TGAAAAGCTGCAGGCTCTGGGGCCGGTAACGCTGCCGGAGAAAATCACGCTGGCGGTGTTTGCCCTGCTGTTGGTGCTGT
GGGCTGGTATTCCGGCGATGATCTTCGGCCCGGCACTGGCGGTTAACCCAACCACGGCGGCGCTGATTGGTCTGGCGGTG
TTGCTCGCAACCGGGGTATTGAGCTGGGAAGATGTGCTCAGGCACAAGGGGGCCTGGGACACGGTGGTGTGGTTCTCCGC
GTTGGTGATGATGGCCAGCTTCCTCGGCAAGTTGGGGCTGATTGGTTGGCTGTCGCAAACCGTGGGCAACGGTATTGATC
ATATGGGAATGAGTTGGGTTGGCGGCACCATCCTGTTGACCCTTATCTATCTGTATTCGCATTACTTCTTTGCCAGCACC
ACCGCACACGTTACCGCCATGTTTGCCGCGTTCTTTGCCGCCGGCATTGCGCTGGGTGCTCCACCCGCGTTGCTGGGCTT
GATCCTGGCGTTCTCTTCCTCGCTGATGATGTCGCTGACCCACTACGGTACCGGCACGGCACCGATTGTCTTTGGCTCCG
GCTATGCGACCTTGGGTGAGTGGTGGAAAGCCGGTTTTGTGATGAGCGTGGTCAACCTGCTGATTTGGATATTGATTGGT
GGAGCGTGGTGGAAGTGGCTGGGCTACTGGTAA

Upstream 100 bases:

>100_bases
TACGCCCGCCCCCCAAGCTGAACGGACCCGGAACGCGATAAGTGCGTTCCCGCCCATCTTGCTGCCAAGATGCCGATCCG
TTTAGAAAAGTGGGTATGTT

Downstream 100 bases:

>100_bases
GCGGTTATTAATAATAAAAAAACACCCGCCAGGTGGCGGGTGATTAATATCAGTTCAGGAAGGCGGGTTGCTGCGCCTCG
TAGCGGGAAATATCCGCTTC

Product: anion transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MDKLTPLKPVPSLCAVAAALLIWFVIPVPEGVAPNAWHLLALFIGTIIAIIGKAMPIGAVSVVAIALVAVTGVTNPGKPG
AALNDALSGFSNQLIWLIGFSIMISLSLNKTGLGARIGYYFISLFGKKTLGIAYALTLAETTLAPVTPSNTARGGGIIHP
IMKSIADSFGSKPELNTSGKIGRYLSLVNYNINPITSAMFITATAPNPLIVSLIAKGTHGNFELSWSMWAVAALVPGLCS
LIVMPLVIYLLYPPEVKSTPDAPRFAREKLQALGPVTLPEKITLAVFALLLVLWAGIPAMIFGPALAVNPTTAALIGLAV
LLATGVLSWEDVLRHKGAWDTVVWFSALVMMASFLGKLGLIGWLSQTVGNGIDHMGMSWVGGTILLTLIYLYSHYFFAST
TAHVTAMFAAFFAAGIALGAPPALLGLILAFSSSLMMSLTHYGTGTAPIVFGSGYATLGEWWKAGFVMSVVNLLIWILIG
GAWWKWLGYW

Sequences:

>Translated_490_residues
MDKLTPLKPVPSLCAVAAALLIWFVIPVPEGVAPNAWHLLALFIGTIIAIIGKAMPIGAVSVVAIALVAVTGVTNPGKPG
AALNDALSGFSNQLIWLIGFSIMISLSLNKTGLGARIGYYFISLFGKKTLGIAYALTLAETTLAPVTPSNTARGGGIIHP
IMKSIADSFGSKPELNTSGKIGRYLSLVNYNINPITSAMFITATAPNPLIVSLIAKGTHGNFELSWSMWAVAALVPGLCS
LIVMPLVIYLLYPPEVKSTPDAPRFAREKLQALGPVTLPEKITLAVFALLLVLWAGIPAMIFGPALAVNPTTAALIGLAV
LLATGVLSWEDVLRHKGAWDTVVWFSALVMMASFLGKLGLIGWLSQTVGNGIDHMGMSWVGGTILLTLIYLYSHYFFAST
TAHVTAMFAAFFAAGIALGAPPALLGLILAFSSSLMMSLTHYGTGTAPIVFGSGYATLGEWWKAGFVMSVVNLLIWILIG
GAWWKWLGYW
>Mature_490_residues
MDKLTPLKPVPSLCAVAAALLIWFVIPVPEGVAPNAWHLLALFIGTIIAIIGKAMPIGAVSVVAIALVAVTGVTNPGKPG
AALNDALSGFSNQLIWLIGFSIMISLSLNKTGLGARIGYYFISLFGKKTLGIAYALTLAETTLAPVTPSNTARGGGIIHP
IMKSIADSFGSKPELNTSGKIGRYLSLVNYNINPITSAMFITATAPNPLIVSLIAKGTHGNFELSWSMWAVAALVPGLCS
LIVMPLVIYLLYPPEVKSTPDAPRFAREKLQALGPVTLPEKITLAVFALLLVLWAGIPAMIFGPALAVNPTTAALIGLAV
LLATGVLSWEDVLRHKGAWDTVVWFSALVMMASFLGKLGLIGWLSQTVGNGIDHMGMSWVGGTILLTLIYLYSHYFFAST
TAHVTAMFAAFFAAGIALGAPPALLGLILAFSSSLMMSLTHYGTGTAPIVFGSGYATLGEWWKAGFVMSVVNLLIWILIG
GAWWKWLGYW

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1786986, Length=467, Percent_Identity=36.6167023554604, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1786829, Length=479, Percent_Identity=31.9415448851775, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789444, Length=474, Percent_Identity=33.3333333333333, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 51824; Mature: 51824

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLTPLKPVPSLCAVAAALLIWFVIPVPEGVAPNAWHLLALFIGTIIAIIGKAMPIGAV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
SVVAIALVAVTGVTNPGKPGAALNDALSGFSNQLIWLIGFSIMISLSLNKTGLGARIGYY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
FISLFGKKTLGIAYALTLAETTLAPVTPSNTARGGGIIHPIMKSIADSFGSKPELNTSGK
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
IGRYLSLVNYNINPITSAMFITATAPNPLIVSLIAKGTHGNFELSWSMWAVAALVPGLCS
HHHHHHHHCCCCCCCCCEEEEEECCCCCEEEHEECCCCCCCEEEHHHHHHHHHHHHHHHH
LIVMPLVIYLLYPPEVKSTPDAPRFAREKLQALGPVTLPEKITLAVFALLLVLWAGIPAM
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHH
IFGPALAVNPTTAALIGLAVLLATGVLSWEDVLRHKGAWDTVVWFSALVMMASFLGKLGL
HHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGWLSQTVGNGIDHMGMSWVGGTILLTLIYLYSHYFFASTTAHVTAMFAAFFAAGIALGA
HHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPALLGLILAFSSSLMMSLTHYGTGTAPIVFGSGYATLGEWWKAGFVMSVVNLLIWILIG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAWWKWLGYW
HHHHHHHCCC
>Mature Secondary Structure
MDKLTPLKPVPSLCAVAAALLIWFVIPVPEGVAPNAWHLLALFIGTIIAIIGKAMPIGAV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
SVVAIALVAVTGVTNPGKPGAALNDALSGFSNQLIWLIGFSIMISLSLNKTGLGARIGYY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
FISLFGKKTLGIAYALTLAETTLAPVTPSNTARGGGIIHPIMKSIADSFGSKPELNTSGK
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
IGRYLSLVNYNINPITSAMFITATAPNPLIVSLIAKGTHGNFELSWSMWAVAALVPGLCS
HHHHHHHHCCCCCCCCCEEEEEECCCCCEEEHEECCCCCCCEEEHHHHHHHHHHHHHHHH
LIVMPLVIYLLYPPEVKSTPDAPRFAREKLQALGPVTLPEKITLAVFALLLVLWAGIPAM
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHH
IFGPALAVNPTTAALIGLAVLLATGVLSWEDVLRHKGAWDTVVWFSALVMMASFLGKLGL
HHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGWLSQTVGNGIDHMGMSWVGGTILLTLIYLYSHYFFASTTAHVTAMFAAFFAAGIALGA
HHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPALLGLILAFSSSLMMSLTHYGTGTAPIVFGSGYATLGEWWKAGFVMSVVNLLIWILIG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAWWKWLGYW
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]