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The map label for this gene is kefF [H]

Identifier: 157368960

GI number: 157368960

Start: 792631

End: 793188

Strand: Direct

Name: kefF [H]

Synonym: Spro_0715

Alternate gene names: 157368960

Gene position: 792631-793188 (Clockwise)

Preceding gene: 157368959

Following gene: 157368961

Centisome position: 14.55

GC content: 56.81

Gene sequence:

>558_bases
ATGATCCTGATAATTTACGCCCATCCTTATCCCCGGCATTCTCATGCCAACCACCGCCTGTTACAGGCGGTCAAGGATCT
TCCCGAGGTGGAAGTCCGTTCGCTGTATGAGCTGTATCCCGATTTCAATATCGATATCAACGCCGAACAGCAAGCGCTGG
AGCGTGCCGACCTGGTGGTGCTGCAGCATCCGATGCAGTGGTACAGCCAACCGCCGTTGCTGAAACTGTGGATCGACAAA
GTGCTGGAGCACGGTTGGGCCTATGGCCACGAAGGCGATGCCCTGGTAGGCAAAGATCTGCTGTGGGCGGTAACCAGCGG
TGGCGATGAGCACCATTTCGAACTGGGCGATTACCCGAATTTTGCCGCTCTGGGGCAGCCGCTGCAGGCCACCGCGCTGT
ATTGCGGTATGCATTGGCAGCCGTATTTTGCGGTGCATAATACTTTTACCTGCAATGAACCGGCGCTGATTGCCGCCGGA
GAAGCCTACCGGCGGCGGCTGGTGGATTACCTGATGGAACACAGTGAACCGGCGGAGCAGGGAGTAGAACATGGATAA

Upstream 100 bases:

>100_bases
GCGTTAAAAATTTCCGCTATCAATCATTCAGCAAACTGTCACTCTGTGTGATTGGTATATGCTTGATATATTGGCAAGGA
ACGAAGGAACGACGACAACC

Downstream 100 bases:

>100_bases
TCACCATATGATGATTGAGGGGCTGATCTATCTGGGATCGGCCGCGCTGTTCGTGCCGATCGCGGTGCGGCTGGGGTTGG
GCTCGGTACTGGGTTATCTG

Product: glutathione-regulated potassium-efflux system ancillary protein KefF

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MILIIYAHPYPRHSHANHRLLQAVKDLPEVEVRSLYELYPDFNIDINAEQQALERADLVVLQHPMQWYSQPPLLKLWIDK
VLEHGWAYGHEGDALVGKDLLWAVTSGGDEHHFELGDYPNFAALGQPLQATALYCGMHWQPYFAVHNTFTCNEPALIAAG
EAYRRRLVDYLMEHSEPAEQGVEHG

Sequences:

>Translated_185_residues
MILIIYAHPYPRHSHANHRLLQAVKDLPEVEVRSLYELYPDFNIDINAEQQALERADLVVLQHPMQWYSQPPLLKLWIDK
VLEHGWAYGHEGDALVGKDLLWAVTSGGDEHHFELGDYPNFAALGQPLQATALYCGMHWQPYFAVHNTFTCNEPALIAAG
EAYRRRLVDYLMEHSEPAEQGVEHG
>Mature_185_residues
MILIIYAHPYPRHSHANHRLLQAVKDLPEVEVRSLYELYPDFNIDINAEQQALERADLVVLQHPMQWYSQPPLLKLWIDK
VLEHGWAYGHEGDALVGKDLLWAVTSGGDEHHFELGDYPNFAALGQPLQATALYCGMHWQPYFAVHNTFTCNEPALIAAG
EAYRRRLVDYLMEHSEPAEQGVEHG

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786231, Length=174, Percent_Identity=65.5172413793103, Blast_Score=242, Evalue=1e-65,
Organism=Escherichia coli, GI1789750, Length=141, Percent_Identity=41.8439716312057, Blast_Score=100, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.- [C]

Molecular weight: Translated: 21179; Mature: 21179

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIIYAHPYPRHSHANHRLLQAVKDLPEVEVRSLYELYPDFNIDINAEQQALERADLVV
CEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
LQHPMQWYSQPPLLKLWIDKVLEHGWAYGHEGDALVGKDLLWAVTSGGDEHHFELGDYPN
EECCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEECCCCCCCEECCCCCC
FAALGQPLQATALYCGMHWQPYFAVHNTFTCNEPALIAAGEAYRRRLVDYLMEHSEPAEQ
HHHHCCCHHHHHHHHCCCCCEEEEECCEEECCCCCEEECCHHHHHHHHHHHHHCCCCHHC
GVEHG
CCCCC
>Mature Secondary Structure
MILIIYAHPYPRHSHANHRLLQAVKDLPEVEVRSLYELYPDFNIDINAEQQALERADLVV
CEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
LQHPMQWYSQPPLLKLWIDKVLEHGWAYGHEGDALVGKDLLWAVTSGGDEHHFELGDYPN
EECCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEECCCCCCCEECCCCCC
FAALGQPLQATALYCGMHWQPYFAVHNTFTCNEPALIAAGEAYRRRLVDYLMEHSEPAEQ
HHHHCCCHHHHHHHHCCCCCEEEEECCEEECCCCCEEECCHHHHHHHHHHHHHCCCCHHC
GVEHG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA