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The map label for this gene is lasT [H]

Identifier: 157368927

GI number: 157368927

Start: 749763

End: 750449

Strand: Direct

Name: lasT [H]

Synonym: Spro_0682

Alternate gene names: 157368927

Gene position: 749763-750449 (Clockwise)

Preceding gene: 157368925

Following gene: 157368928

Centisome position: 13.76

GC content: 57.35

Gene sequence:

>687_bases
ATGCAGCTTCATATTGTTTTAGTGGCTCCGGCGCGGCCGGAAAACGTCGGCGCTGCGGCGCGGGCAATGAAAACCATGGG
TTTCACTTCGTTGCGCATTGTCGACAGCGAAGCGCACCTGCAACCGGCGGCTCGCTGGGTGGCTCATGGGGCGGGGGATA
TCCTCGACGGCGTGCAGACTTTCGCCACTCTCGAGCAGGCGCTGGCGGACGTGGATTTCACCGTTGCTACCACTGCCCGC
AGCCGCGCGCGTTTCCATTATTACTGTACGCCGCAGCAACTGTTGGGTCAGTTGAGTGAACGCAGTCAGTGGGTGGGGCA
GGCAGCGTTGGTGTTTGGCCGTGAAGATTCCGGCCTGACCAATGAAGAGTTGGAGTTGGCAGATTTGCTGACCGGTGTGC
CGATGCAGGCAGATTACCCTTCGTTGAATCTCGGCCAGGCGGTGATGGTGTATTGCTATCAATTGTCGGAACTGATGAAG
GTTAGCGCACCGCAGGAACCAGTGGCTAATGAGGGCCAGCTTAAAGCACTGCGTCAGCGTGCCGATGGTTTGCTGGGGGC
ATTGGGGGTGGGTGACGATCAAAAATTGCGCGATTGGCTGCACCAGCGGCTTGGTACCTTGCAGCAGCGCGATACGGCAA
TGTTGCATACTTTGCTGCATGACATCGAAAAAAAACTGACAAAGTGA

Upstream 100 bases:

>100_bases
GTCTACCCTCAATTTCGGGATTATTGTTGCGTAGATGTAAATATAATCAGACTATCCCAAACAGTCAAAAATACGCGATC
TGTCGTTTAGTGGAGTATTG

Downstream 100 bases:

>100_bases
TACCCGCAAACATGGCTAATTAGGCAGTGTGATCCACTGCAAAACTGCCGTCTGTCTGTCTTTTTATCCTGCTATTTGGC
TTTTCGACTGTCGCATTGCC

Product: putative RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MQLHIVLVAPARPENVGAAARAMKTMGFTSLRIVDSEAHLQPAARWVAHGAGDILDGVQTFATLEQALADVDFTVATTAR
SRARFHYYCTPQQLLGQLSERSQWVGQAALVFGREDSGLTNEELELADLLTGVPMQADYPSLNLGQAVMVYCYQLSELMK
VSAPQEPVANEGQLKALRQRADGLLGALGVGDDQKLRDWLHQRLGTLQQRDTAMLHTLLHDIEKKLTK

Sequences:

>Translated_228_residues
MQLHIVLVAPARPENVGAAARAMKTMGFTSLRIVDSEAHLQPAARWVAHGAGDILDGVQTFATLEQALADVDFTVATTAR
SRARFHYYCTPQQLLGQLSERSQWVGQAALVFGREDSGLTNEELELADLLTGVPMQADYPSLNLGQAVMVYCYQLSELMK
VSAPQEPVANEGQLKALRQRADGLLGALGVGDDQKLRDWLHQRLGTLQQRDTAMLHTLLHDIEKKLTK
>Mature_228_residues
MQLHIVLVAPARPENVGAAARAMKTMGFTSLRIVDSEAHLQPAARWVAHGAGDILDGVQTFATLEQALADVDFTVATTAR
SRARFHYYCTPQQLLGQLSERSQWVGQAALVFGREDSGLTNEELELADLLTGVPMQADYPSLNLGQAVMVYCYQLSELMK
VSAPQEPVANEGQLKALRQRADGLLGALGVGDDQKLRDWLHQRLGTLQQRDTAMLHTLLHDIEKKLTK

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=228, Percent_Identity=69.7368421052632, Blast_Score=335, Evalue=1e-93,
Organism=Escherichia coli, GI1788881, Length=155, Percent_Identity=38.0645161290323, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25058; Mature: 25058

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLHIVLVAPARPENVGAAARAMKTMGFTSLRIVDSEAHLQPAARWVAHGAGDILDGVQT
CEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
FATLEQALADVDFTVATTARSRARFHYYCTPQQLLGQLSERSQWVGQAALVFGREDSGLT
HHHHHHHHHHCCHHEEHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
NEELELADLLTGVPMQADYPSLNLGQAVMVYCYQLSELMKVSAPQEPVANEGQLKALRQR
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
ADGLLGALGVGDDQKLRDWLHQRLGTLQQRDTAMLHTLLHDIEKKLTK
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLHIVLVAPARPENVGAAARAMKTMGFTSLRIVDSEAHLQPAARWVAHGAGDILDGVQT
CEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
FATLEQALADVDFTVATTARSRARFHYYCTPQQLLGQLSERSQWVGQAALVFGREDSGLT
HHHHHHHHHHCCHHEEHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
NEELELADLLTGVPMQADYPSLNLGQAVMVYCYQLSELMKVSAPQEPVANEGQLKALRQR
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
ADGLLGALGVGDDQKLRDWLHQRLGTLQQRDTAMLHTLLHDIEKKLTK
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]