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The map label for this gene is ascG [H]

Identifier: 157368822

GI number: 157368822

Start: 641536

End: 642570

Strand: Direct

Name: ascG [H]

Synonym: Spro_0577

Alternate gene names: 157368822

Gene position: 641536-642570 (Clockwise)

Preceding gene: 157368813

Following gene: 157368824

Centisome position: 11.77

GC content: 59.32

Gene sequence:

>1035_bases
ATGGCAACAATACTTGAGGTAGCGAACAGGGCGGGCGTGTCGAAAGCGACGGTGTCCCGCGTGTTGTCAGGCAGCGGCTA
CGTCAGCCCGGAGAAGCGTGAACGGGTGGATAAAGCCATCGCCGAAACGGGTTACCGGCCCAATCTGCTGGCGCGCAATC
TGGCGACCAAAACCACCCAGACCATCGGGCTGGTGGTGACCAACACGCTGTACAGCGGTAACTACTTCAGCGAGCTGATG
TTTCAGTCGGCGCGCATCATGGAAGAGCACGGCCGCCAACTGATTTTGGCGGACGGCAAGCACACCGCCGCAGAAGAAAA
AGCCGCCATTGAATACCTGCTGGATATGCGCTGCGACGGCGTGATCATCTACCCGCGTTTTCTGTCGATTAGCGAAATGG
ACGAGATCATCTCCCGGCACAAGCAGCCGATCCTGGTCATCAACCGTCGGCTCAGGCTGAACGACAGCTACTGCATTTTC
AGCGATCAACACGCCGCCAGCGTGACGGCGGTGCAGCAGCTGATCGAGCGGGGGCATCGCGATATCGCTTTTATCACCGG
CTCGCTGGATTCCCCTACCGGGCTGGAGCGGCTCTCAGGCTACAAATCTGCGCTGGCGGAACATGATATTGGCGTAAAAG
ACGCGCTGATTGTCGAAGGGAAGTGGAACGCGCAAAGCGGCATGGCGGCGGTCGATAAGCTGTTGGCCGGCGGCCACGCC
TTTAGCGCGATTGTTGCCAGCAACGACGAGATGGCCATCGGCGCCATCAAGCGGCTGACGGAATGCCAAATCCCGGTGCC
GTCCGCCGTGTCGGTGATAGGTTTTGATGATATTCCGCTGGCCCCTTATACCATTCCCTCACTCTCCAGCGTGAAAATGC
CGGTGTCCGAAATGATTAAGGAAACCATCCATCGGCTGGTATCGATGCTGGACGGCGGCGAACTGAGCAAGCGCCCCAGC
TTCCCGGCCAGCCTGATGCTGCGTGATTCGGTGGCGGCGGGGCCTTATTGGTTGGCAGAGAAGGCGCGCGGTTAA

Upstream 100 bases:

>100_bases
TTTGGTTCAAGGGTGGCGACGGGAGCGGGCGTGAAAACATTTCAAACCGGGGGCGTCTTGGGTAGACTGCGCTGACTCAA
TCGACTTTGACGGCGCAGAG

Downstream 100 bases:

>100_bases
CGGCGGATCAGCATATAGGCGCGCGGCGAACTGCCGGTCATATTTTTAAAGAAGGTTGAGAAGGCGCTGTCGCTGGAGAA
TTCAAGCTTACCGGCGATAT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MATILEVANRAGVSKATVSRVLSGSGYVSPEKRERVDKAIAETGYRPNLLARNLATKTTQTIGLVVTNTLYSGNYFSELM
FQSARIMEEHGRQLILADGKHTAAEEKAAIEYLLDMRCDGVIIYPRFLSISEMDEIISRHKQPILVINRRLRLNDSYCIF
SDQHAASVTAVQQLIERGHRDIAFITGSLDSPTGLERLSGYKSALAEHDIGVKDALIVEGKWNAQSGMAAVDKLLAGGHA
FSAIVASNDEMAIGAIKRLTECQIPVPSAVSVIGFDDIPLAPYTIPSLSSVKMPVSEMIKETIHRLVSMLDGGELSKRPS
FPASLMLRDSVAAGPYWLAEKARG

Sequences:

>Translated_344_residues
MATILEVANRAGVSKATVSRVLSGSGYVSPEKRERVDKAIAETGYRPNLLARNLATKTTQTIGLVVTNTLYSGNYFSELM
FQSARIMEEHGRQLILADGKHTAAEEKAAIEYLLDMRCDGVIIYPRFLSISEMDEIISRHKQPILVINRRLRLNDSYCIF
SDQHAASVTAVQQLIERGHRDIAFITGSLDSPTGLERLSGYKSALAEHDIGVKDALIVEGKWNAQSGMAAVDKLLAGGHA
FSAIVASNDEMAIGAIKRLTECQIPVPSAVSVIGFDDIPLAPYTIPSLSSVKMPVSEMIKETIHRLVSMLDGGELSKRPS
FPASLMLRDSVAAGPYWLAEKARG
>Mature_343_residues
ATILEVANRAGVSKATVSRVLSGSGYVSPEKRERVDKAIAETGYRPNLLARNLATKTTQTIGLVVTNTLYSGNYFSELMF
QSARIMEEHGRQLILADGKHTAAEEKAAIEYLLDMRCDGVIIYPRFLSISEMDEIISRHKQPILVINRRLRLNDSYCIFS
DQHAASVTAVQQLIERGHRDIAFITGSLDSPTGLERLSGYKSALAEHDIGVKDALIVEGKWNAQSGMAAVDKLLAGGHAF
SAIVASNDEMAIGAIKRLTECQIPVPSAVSVIGFDDIPLAPYTIPSLSSVKMPVSEMIKETIHRLVSMLDGGELSKRPSF
PASLMLRDSVAAGPYWLAEKARG

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=62.4624624624625, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=35.9281437125749, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=32.7327327327327, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=30.791788856305, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=32.1766561514196, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=30.0884955752212, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=29.7805642633229, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=30.3571428571429, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1787906, Length=302, Percent_Identity=28.1456953642384, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=26.0869565217391, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790715, Length=302, Percent_Identity=23.841059602649, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=24.9230769230769, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37460; Mature: 37328

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATILEVANRAGVSKATVSRVLSGSGYVSPEKRERVDKAIAETGYRPNLLARNLATKTTQ
CCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
TIGLVVTNTLYSGNYFSELMFQSARIMEEHGRQLILADGKHTAAEEKAAIEYLLDMRCDG
HEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
VIIYPRFLSISEMDEIISRHKQPILVINRRLRLNDSYCIFSDQHAASVTAVQQLIERGHR
EEEEECCCCHHHHHHHHHHCCCCEEEEECEEEECCCEEEECCCHHHHHHHHHHHHHCCCC
DIAFITGSLDSPTGLERLSGYKSALAEHDIGVKDALIVEGKWNAQSGMAAVDKLLAGGHA
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCH
FSAIVASNDEMAIGAIKRLTECQIPVPSAVSVIGFDDIPLAPYTIPSLSSVKMPVSEMIK
HEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
ETIHRLVSMLDGGELSKRPSFPASLMLRDSVAAGPYWLAEKARG
HHHHHHHHHHCCCCCCCCCCCCHHHEEHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
ATILEVANRAGVSKATVSRVLSGSGYVSPEKRERVDKAIAETGYRPNLLARNLATKTTQ
CHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
TIGLVVTNTLYSGNYFSELMFQSARIMEEHGRQLILADGKHTAAEEKAAIEYLLDMRCDG
HEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
VIIYPRFLSISEMDEIISRHKQPILVINRRLRLNDSYCIFSDQHAASVTAVQQLIERGHR
EEEEECCCCHHHHHHHHHHCCCCEEEEECEEEECCCEEEECCCHHHHHHHHHHHHHCCCC
DIAFITGSLDSPTGLERLSGYKSALAEHDIGVKDALIVEGKWNAQSGMAAVDKLLAGGHA
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCH
FSAIVASNDEMAIGAIKRLTECQIPVPSAVSVIGFDDIPLAPYTIPSLSSVKMPVSEMIK
HEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
ETIHRLVSMLDGGELSKRPSFPASLMLRDSVAAGPYWLAEKARG
HHHHHHHHHHCCCCCCCCCCCCHHHEEHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]