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The map label for this gene is emrD [C]

Identifier: 157368824

GI number: 157368824

Start: 643439

End: 644626

Strand: Direct

Name: emrD [C]

Synonym: Spro_0579

Alternate gene names: 157368824

Gene position: 643439-644626 (Clockwise)

Preceding gene: 157368822

Following gene: 157368830

Centisome position: 11.81

GC content: 61.11

Gene sequence:

>1188_bases
ATGCAACGCAGACTGACCCTTACCCTGGCGACACTGATGATGATGTTTCCCCAAATTGTGGAAACGATCTATAGCCCGGC
GCTCACGCACATTGCCGACGGTTTTCACGTCAGCGCGGAGGTCGCGGCGCAAACCCTGTCCTGTTACTTTTTCGCTTTTG
CTTTCGGGGTTGTCATCTGGGGCCGTTTGTGTGACGTGATCGGCCGGCGCCCCACCATTTTGGCCGGTTTGGCGCTGTAT
CTGCTGGCGTCGATCATCGCCATCTTCAGCACCGGCTTTACCCTGCTGTTGGCGGCCAGGGTGCTGGCGGCGTTTGGTGC
AGCGGTGGGGTCCGTAGGCACCCAGACTGCCATTCGCGATCGGTTCGACGGTTATGAGCTGGCGCGGGTGTTTTCCGTGA
TGGGGATTGCGATGGCGGTCAGCCCTGCGGTGGGGGTGCTGAGCGGCGCCCTGTTAACCCATTACTGGGGTTATCAGGGG
GTGTTTGCCGGGTTGGCGGCGTTGGCCGCCTTGCTGCTGGCCTATGCCGCATGGCAGTTGCCGGAAACGCGCCCGGCGCA
GGTGGTGAAAAGCTCCTTCTTCGGCACGTTTGGCAGAATGGTGAAAGACGGCGATATCTGGCGCAGCGCATGGCTGGTGG
CCCTGTTCAACGTCTGCATGTTCAGCTATTACCAACTGGCACCGTTTCGGTTTGAAGCGCTGGGCCTTCCGCCGCAGTGG
TTTGGCTATACCGGGTTGGTGCTGGCCGCCGGCGTCGGGATCGGCGCGGCAATCAACAAGTTTCTCATCGCCAAACAGTG
GCTGTTTACGCCACTGCTGATGCTGGCCTGCGTATTGGCGTTGATCGGCGGCGGCCTGGTGTTCCTGTTGGAAGATAAGC
TGTGGTTCGTGCTGCCGATGATGCTGGTGGTGATGGCCTACGGCATCGCGATCCCAAATATTCTGGCGCGGGCGCTGCGA
AATTATAAGGATTGCATGGGCACCGCGGGCGCAATCCTTGGCCTGATGTACTACCTGATGCTGGCGGCGGGGCTGGTGCT
GGCCGGGTTGGCGCAGCGGCCGGGCATCGTACTGATGACGAGTAGCGCCTGCGCGCTGCTGCTGGCGTTGACCGTGGTGC
GCGGTGAACGCAATATCGCTATCTCAACCCAACCCGGCAAACCGCTCCCGGATCGTCTCCTCCGGTAA

Upstream 100 bases:

>100_bases
CTGTCCGATTTTATAAGTTTTTTGTCTTTTATATTGTAATGGTCAGTGTGGGGAAGGTGCTACTTTGTGGCCTACTTTCT
TTTTGGATACGGGATGTACC

Downstream 100 bases:

>100_bases
GTCCACGCCAATAAACACCAGCGTACTGCGGCGTTCCTCGTCGGGCAGCCACTCGCGGTCCCAGTCGGCGTTATACAGCC
GTTGCACGCCCTGGAACAGC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MQRRLTLTLATLMMMFPQIVETIYSPALTHIADGFHVSAEVAAQTLSCYFFAFAFGVVIWGRLCDVIGRRPTILAGLALY
LLASIIAIFSTGFTLLLAARVLAAFGAAVGSVGTQTAIRDRFDGYELARVFSVMGIAMAVSPAVGVLSGALLTHYWGYQG
VFAGLAALAALLLAYAAWQLPETRPAQVVKSSFFGTFGRMVKDGDIWRSAWLVALFNVCMFSYYQLAPFRFEALGLPPQW
FGYTGLVLAAGVGIGAAINKFLIAKQWLFTPLLMLACVLALIGGGLVFLLEDKLWFVLPMMLVVMAYGIAIPNILARALR
NYKDCMGTAGAILGLMYYLMLAAGLVLAGLAQRPGIVLMTSSACALLLALTVVRGERNIAISTQPGKPLPDRLLR

Sequences:

>Translated_395_residues
MQRRLTLTLATLMMMFPQIVETIYSPALTHIADGFHVSAEVAAQTLSCYFFAFAFGVVIWGRLCDVIGRRPTILAGLALY
LLASIIAIFSTGFTLLLAARVLAAFGAAVGSVGTQTAIRDRFDGYELARVFSVMGIAMAVSPAVGVLSGALLTHYWGYQG
VFAGLAALAALLLAYAAWQLPETRPAQVVKSSFFGTFGRMVKDGDIWRSAWLVALFNVCMFSYYQLAPFRFEALGLPPQW
FGYTGLVLAAGVGIGAAINKFLIAKQWLFTPLLMLACVLALIGGGLVFLLEDKLWFVLPMMLVVMAYGIAIPNILARALR
NYKDCMGTAGAILGLMYYLMLAAGLVLAGLAQRPGIVLMTSSACALLLALTVVRGERNIAISTQPGKPLPDRLLR
>Mature_395_residues
MQRRLTLTLATLMMMFPQIVETIYSPALTHIADGFHVSAEVAAQTLSCYFFAFAFGVVIWGRLCDVIGRRPTILAGLALY
LLASIIAIFSTGFTLLLAARVLAAFGAAVGSVGTQTAIRDRFDGYELARVFSVMGIAMAVSPAVGVLSGALLTHYWGYQG
VFAGLAALAALLLAYAAWQLPETRPAQVVKSSFFGTFGRMVKDGDIWRSAWLVALFNVCMFSYYQLAPFRFEALGLPPQW
FGYTGLVLAAGVGIGAAINKFLIAKQWLFTPLLMLACVLALIGGGLVFLLEDKLWFVLPMMLVVMAYGIAIPNILARALR
NYKDCMGTAGAILGLMYYLMLAAGLVLAGLAQRPGIVLMTSSACALLLALTVVRGERNIAISTQPGKPLPDRLLR

Specific function: Multidrug Resistance Pump That Participates In A Low Energy Shock Adaptative Response. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=181, Percent_Identity=28.7292817679558, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994889, Length=352, Percent_Identity=26.4204545454545, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790146, Length=186, Percent_Identity=27.9569892473118, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788509, Length=245, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322071, Length=189, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42531; Mature: 42531

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCC
>Mature Secondary Structure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CHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA