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The map label for this gene is pnp [H]

Identifier: 157368740

GI number: 157368740

Start: 544235

End: 546352

Strand: Direct

Name: pnp [H]

Synonym: Spro_0493

Alternate gene names: 157368740

Gene position: 544235-546352 (Clockwise)

Preceding gene: 157368739

Following gene: 157368741

Centisome position: 9.99

GC content: 54.91

Gene sequence:

>2118_bases
TTGCTGACACCGATCATTCGCAAATTCCAGTATGGCCAGCATACCCTCACTATTGAGACCGGTATGATGGCTCGTCAGGC
CACTGCCGCCGTTATGGTAAGCATGGATGACACCGCAGTATTCGTTACCGTAGTTGGCCAGAAAAAAGCCAAACCAGGCC
AGAGCTTCTTCCCACTGACGGTTAACTATCAGGAGCGTACCTACGCTGCTGGTCGTATCCCGGGTAGCTTCTTCCGTCGT
GAAGGCCGTCCGAGCGAAGGTGAAACCCTGACCTCCCGTCTGATTGACCGTCCGATCCGTCCACTGTTCCCGGACAGCTT
CCTGAACGAAGTTCAGGTGATCGCGACCGTTGTTTCCCTTAACCCGCAGGTTAACCCGGACATCGTTGCGATGATCGGTG
CCTCTGCCGCCCTGAGCCTGTCCGGTATTCCGTTCAATGGCCCAATCGGTTCTGCGCGCGTGGGTTACATCAACAATCAA
TACGTATTGAACCCAACCAGCGACGAACTGAAAGAAAGCAGCCTGGATCTGGTGGTTGCCGGTACCGCTGGCGCAGTACT
GATGGTTGAATCCGAAGCTGACGTTCTGAGCGAAGATCAGATGCTGGGCGCGGTGGTGTTTGGCCACGAGCAACAGCAAA
TCGTTATCGAAAACATTAATTCCCTGGTTGCCGAAGCCGGCAAAGCCAAGTGGGACTGGCAGGCACCTGCAGTCAACGAA
GCGCTGCACGCGCGCGTTGCAGAACTGGCAGAAGGCCGCCTGGGCGACGCTTATCACATCACCGAAAAACAAGAGCGTTA
CGCTCAGGTTGATGCGATCAAGAGCAGCGTTGTTGAAACCCTGCTGGCACAGGACGAAACCCTGGACGTGTCTGAAATTC
AGGACATCCTGGGTAGCGTTGAGAAAAACGTCGTTCGTAGCCGTGTGCTGCGTGGCGAGCCGCGTATCGACGGCCGTGAA
AAAGACATGATCCGTGGTCTGGACGTGCGCACCGGCGTTCTGCCGCGTACCCACGGTTCCGCACTGTTCACCCGTGGTGA
GACTCAGGCACTGGTTACCGCAACGCTGGGCACCGCCCGTGACGCGCAGAACCTGGATGAGCTGATGGGCGAAAAGACCG
ACAGCTTCCTGTTCCACTATAACTTCCCTCCGTACTCCGTTGGTGAGACCGGGATGGTAGGTTCGCCAAAACGTCGTGAA
ATTGGTCACGGTCGCCTGGCGAAACGTGGCGTATTGGCTATGATGCCTAAACCAGAAGATTTCCCGTACACGGTGCGTGT
GGTTTCTGAAATCACCGAATCCAACGGTTCTTCTTCAATGGCTTCCGTCTGTGGTGCTTCTCTGGCACTGATGGATGCAG
GTGTGCCAATCAAGGCCGCCGTTGCCGGTATCGCAATGGGCCTGGTGAAAGAACAAGACAACTTTGTTGTTCTGTCCGAC
ATTCTGGGTGACGAAGATCACCTGGGCGACATGGACTTCAAAGTAGCCGGTAGCCGTGACGGTATTACCGCGCTGCAGAT
GGACATTAAAATTGAAGGCATCACCCGCGAAATCATGCAGGTTGCTCTGAACCAGGCCAAGGGCGCGCGTCTGCACATCC
TGGGCGTGATGGAACAGGCTATCAGCACTCCGCGTGGCGATATCTCTCAGTTTGCACCACGTATTCACACTATCCGCATC
AACCCGGACAAGATCAAAGACGTGATTGGTAAAGGCGGTTCTGTCATCCGTGCGCTGACTGAAGAGACCGGCACTACCAT
TGAAATCGAAGATGATGGTACAGTTAAAATTGCTGCTACCGACGGTGAGAAAGCGAAATTCGCTATCCGCCGCATCGAAG
AGATCACTGCCGAGATCGAAGTGGGCCGTATTTACCAGGGTAAAGTTACCCGTATCGTTGATTTCGGCGCATTCGTGGCG
ATCGGCGGCGGTAAAGAAGGTCTGGTGCACATCTCTCAAATCGCTGACAAGCGCGTTGAGAAAGTGACCGACTATCTGCA
GATGGGTCAGGAAGTACCGGTTAAGGTACTGGAAGTTGACCGTCAGGGCCGTGTGCGTCTGAGCATCAAAGAAGCGACCG
CACCAGAAGCAGGTTCACCTGCGCCTGAAGCAGAATAA

Upstream 100 bases:

>100_bases
AGGATTGTCATTAGTCGCGAGGATGTAGTGAGAAGGCAAACCGAGTCACTGGCGTGTCGAGTCGACAATACGATTGCGCG
CCTAATCTAAGGATATAATT

Downstream 100 bases:

>100_bases
CTGTATAGATAGATTTACAGCTCCCGGCCATGGGGTTGGGAGCTGTTCATATAACGCGGGCAGGATGCCTGCGTATTTGC
AAACGGATGAAAGGATGTTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MLTPIIRKFQYGQHTLTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLTVNYQERTYAAGRIPGSFFRR
EGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSLNPQVNPDIVAMIGASAALSLSGIPFNGPIGSARVGYINNQ
YVLNPTSDELKESSLDLVVAGTAGAVLMVESEADVLSEDQMLGAVVFGHEQQQIVIENINSLVAEAGKAKWDWQAPAVNE
ALHARVAELAEGRLGDAYHITEKQERYAQVDAIKSSVVETLLAQDETLDVSEIQDILGSVEKNVVRSRVLRGEPRIDGRE
KDMIRGLDVRTGVLPRTHGSALFTRGETQALVTATLGTARDAQNLDELMGEKTDSFLFHYNFPPYSVGETGMVGSPKRRE
IGHGRLAKRGVLAMMPKPEDFPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEQDNFVVLSD
ILGDEDHLGDMDFKVAGSRDGITALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQAISTPRGDISQFAPRIHTIRI
NPDKIKDVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKFAIRRIEEITAEIEVGRIYQGKVTRIVDFGAFVA
IGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQGRVRLSIKEATAPEAGSPAPEAE

Sequences:

>Translated_705_residues
MLTPIIRKFQYGQHTLTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLTVNYQERTYAAGRIPGSFFRR
EGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSLNPQVNPDIVAMIGASAALSLSGIPFNGPIGSARVGYINNQ
YVLNPTSDELKESSLDLVVAGTAGAVLMVESEADVLSEDQMLGAVVFGHEQQQIVIENINSLVAEAGKAKWDWQAPAVNE
ALHARVAELAEGRLGDAYHITEKQERYAQVDAIKSSVVETLLAQDETLDVSEIQDILGSVEKNVVRSRVLRGEPRIDGRE
KDMIRGLDVRTGVLPRTHGSALFTRGETQALVTATLGTARDAQNLDELMGEKTDSFLFHYNFPPYSVGETGMVGSPKRRE
IGHGRLAKRGVLAMMPKPEDFPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEQDNFVVLSD
ILGDEDHLGDMDFKVAGSRDGITALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQAISTPRGDISQFAPRIHTIRI
NPDKIKDVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKFAIRRIEEITAEIEVGRIYQGKVTRIVDFGAFVA
IGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQGRVRLSIKEATAPEAGSPAPEAE
>Mature_705_residues
MLTPIIRKFQYGQHTLTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLTVNYQERTYAAGRIPGSFFRR
EGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSLNPQVNPDIVAMIGASAALSLSGIPFNGPIGSARVGYINNQ
YVLNPTSDELKESSLDLVVAGTAGAVLMVESEADVLSEDQMLGAVVFGHEQQQIVIENINSLVAEAGKAKWDWQAPAVNE
ALHARVAELAEGRLGDAYHITEKQERYAQVDAIKSSVVETLLAQDETLDVSEIQDILGSVEKNVVRSRVLRGEPRIDGRE
KDMIRGLDVRTGVLPRTHGSALFTRGETQALVTATLGTARDAQNLDELMGEKTDSFLFHYNFPPYSVGETGMVGSPKRRE
IGHGRLAKRGVLAMMPKPEDFPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEQDNFVVLSD
ILGDEDHLGDMDFKVAGSRDGITALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQAISTPRGDISQFAPRIHTIRI
NPDKIKDVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKFAIRRIEEITAEIEVGRIYQGKVTRIVDFGAFVA
IGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQGRVRLSIKEATAPEAGSPAPEAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=709, Percent_Identity=39.3511988716502, Blast_Score=455, Evalue=1e-128,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=90.04329004329, Blast_Score=1275, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=714, Percent_Identity=34.1736694677871, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17535281, Length=75, Percent_Identity=48, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320850, Length=101, Percent_Identity=33.6633663366337, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=37.883008356546, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=37.883008356546, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=37.883008356546, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI161079377, Length=664, Percent_Identity=37.5, Blast_Score=425, Evalue=1e-119,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76252; Mature: 76252

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPIIRKFQYGQHTLTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLT
CCCHHHHHHHCCCEEEEEECCCHHHHCEEEEEEEECCCEEEEEEECCCCCCCCCCEEEEE
VNYQERTYAAGRIPGSFFRREGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSL
EECCHHEEECCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
NPQVNPDIVAMIGASAALSLSGIPFNGPIGSARVGYINNQYVLNPTSDELKESSLDLVVA
CCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHHCCCCCEEEE
GTAGAVLMVESEADVLSEDQMLGAVVFGHEQQQIVIENINSLVAEAGKAKWDWQAPAVNE
CCCCEEEEEECCCCHHCCCHHEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
ALHARVAELAEGRLGDAYHITEKQERYAQVDAIKSSVVETLLAQDETLDVSEIQDILGSV
HHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EKNVVRSRVLRGEPRIDGREKDMIRGLDVRTGVLPRTHGSALFTRGETQALVTATLGTAR
HHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCC
DAQNLDELMGEKTDSFLFHYNFPPYSVGETGMVGSPKRREIGHGRLAKRGVLAMMPKPED
CHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCCHHHCCEEEECCCCCC
FPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEQDNFVVLSD
CCCHHHHHHHHHHCCCCHHHHHHHHHHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEEEH
ILGDEDHLGDMDFKVAGSRDGITALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQA
HCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCEEEEHHHHHHH
ISTPRGDISQFAPRIHTIRINPDKIKDVIGKGGSVIRALTEETGTTIEIEDDGTVKIAAT
HCCCCCCHHHHCCCEEEEEECHHHHHHHHCCCCCEEHHHHHCCCCEEEECCCCEEEEEEC
DGEKAKFAIRRIEEITAEIEVGRIYQGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVE
CCCHHHHHHHHHHHHHHHEEECEEECCCEEEEEECCEEEEECCCCCCCEEHHHHHHHHHH
KVTDYLQMGQEVPVKVLEVDRQGRVRLSIKEATAPEAGSPAPEAE
HHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTPIIRKFQYGQHTLTIETGMMARQATAAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLT
CCCHHHHHHHCCCEEEEEECCCHHHHCEEEEEEEECCCEEEEEEECCCCCCCCCCEEEEE
VNYQERTYAAGRIPGSFFRREGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSL
EECCHHEEECCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
NPQVNPDIVAMIGASAALSLSGIPFNGPIGSARVGYINNQYVLNPTSDELKESSLDLVVA
CCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHHCCCCCEEEE
GTAGAVLMVESEADVLSEDQMLGAVVFGHEQQQIVIENINSLVAEAGKAKWDWQAPAVNE
CCCCEEEEEECCCCHHCCCHHEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
ALHARVAELAEGRLGDAYHITEKQERYAQVDAIKSSVVETLLAQDETLDVSEIQDILGSV
HHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EKNVVRSRVLRGEPRIDGREKDMIRGLDVRTGVLPRTHGSALFTRGETQALVTATLGTAR
HHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCC
DAQNLDELMGEKTDSFLFHYNFPPYSVGETGMVGSPKRREIGHGRLAKRGVLAMMPKPED
CHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCCHHHCCEEEECCCCCC
FPYTVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEQDNFVVLSD
CCCHHHHHHHHHHCCCCHHHHHHHHHHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEEEH
ILGDEDHLGDMDFKVAGSRDGITALQMDIKIEGITREIMQVALNQAKGARLHILGVMEQA
HCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCEEEEHHHHHHH
ISTPRGDISQFAPRIHTIRINPDKIKDVIGKGGSVIRALTEETGTTIEIEDDGTVKIAAT
HCCCCCCHHHHCCCEEEEEECHHHHHHHHCCCCCEEHHHHHCCCCEEEECCCCEEEEEEC
DGEKAKFAIRRIEEITAEIEVGRIYQGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVE
CCCHHHHHHHHHHHHHHHEEECEEECCCEEEEEECCEEEEECCCCCCCEEHHHHHHHHHH
KVTDYLQMGQEVPVKVLEVDRQGRVRLSIKEATAPEAGSPAPEAE
HHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA