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The map label for this gene is truB

Identifier: 157368738

GI number: 157368738

Start: 542642

End: 543586

Strand: Direct

Name: truB

Synonym: Spro_0491

Alternate gene names: 157368738

Gene position: 542642-543586 (Clockwise)

Preceding gene: 157368737

Following gene: 157368739

Centisome position: 9.96

GC content: 58.73

Gene sequence:

>945_bases
ATGAGTCGCCCTCGTCGCCGCGGCCGTGATATCCACGGTGTCCTGTTGCTGGACAAACCGCAGGGCCTGTCATCCAACGA
CGCCCTGCAAAAAGTAAAGCGCCTCTATAACGCCAATCGCGCGGGTCATACCGGCGCGCTCGACCCGCTGGCGACCGGCA
TGCTGCCAATTTGCCTCGGTGAGGCGACCAAGTTTTCACAGTTCCTGCTCGACTCCGACAAACGCTATCGCGTGATTGCC
CGACTGGGTCAGCGTACCGATACCTCGGATGCCGACGGCCAAATTGTGCAGGAGCGGCCGGTGAGTTTCACCCAGGCGCA
GCTCGATGCGGCGCTGGACAGCTTCCGTGGCGATATCAAGCAGGTGCCTTCGATGTATTCGGCGCTGAAATACCAGGGCA
AGAAACTCTATGAGTATGCTCGCCAGGGTATCGAAGTGCCGCGTGAAGCACGCAGCATTACCGTTTATGAACTGCAGTTT
ATTCGCTGGGAAGGGGATGAACTGGAGCTGGAAATTCACTGCTCAAAAGGCACTTACATCCGCACCATCACCGATGACCT
CGGCGAGCTGCTGGGATGTGGCGCGCACGTGATCTACCTGCGTCGTCTGCAGGTGGCGACCTACCCGACCGAGCGCATGG
TCACGCTGGAGCAGTTGAACGAATTGCTGGAGCAGGCTCATCGGCAGGAAATCGCCCCGGCCGAGCTGCTCGATCCGTTG
TTGATGCCGATGGACAGCCCGGTGGAGAACTACCCGGAAGTGAATTTGCTGCCAGTGGTAGCGGGTTACGTTAAGCAAGG
GCAACCGGTTCAGGTTGCCGGTGCACCGGCCTCGGGCATGGTTCGTATCACCGAAGGTGAAGAGCGTAAATTTATCGGCG
TCGGTGATATTGCCGATGACGGTCGTGTGGCGCCGCGCCGCCTGGTAGTTGAATATTTCGACTAA

Upstream 100 bases:

>100_bases
ACTCCCTGGTTGAAGGCATGCGCATGTCCAACCTGGTGACCAACGTAGTGAAGAATGACGCCGAACGCCGTTCCGCATCA
GGCGATGACGAGGAGGCTTA

Downstream 100 bases:

>100_bases
GGGTAGAAAACGCACTGCCTTGCGATCGCACTGCGCTAAGGGTAGAATGGCGCAGCTTATGCATTGGGTTGCTGAATTAG
AGATCGGCGCCTGTATTTAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSRPRRRGRDIHGVLLLDKPQGLSSNDALQKVKRLYNANRAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVIA
RLGQRTDTSDADGQIVQERPVSFTQAQLDAALDSFRGDIKQVPSMYSALKYQGKKLYEYARQGIEVPREARSITVYELQF
IRWEGDELELEIHCSKGTYIRTITDDLGELLGCGAHVIYLRRLQVATYPTERMVTLEQLNELLEQAHRQEIAPAELLDPL
LMPMDSPVENYPEVNLLPVVAGYVKQGQPVQVAGAPASGMVRITEGEERKFIGVGDIADDGRVAPRRLVVEYFD

Sequences:

>Translated_314_residues
MSRPRRRGRDIHGVLLLDKPQGLSSNDALQKVKRLYNANRAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVIA
RLGQRTDTSDADGQIVQERPVSFTQAQLDAALDSFRGDIKQVPSMYSALKYQGKKLYEYARQGIEVPREARSITVYELQF
IRWEGDELELEIHCSKGTYIRTITDDLGELLGCGAHVIYLRRLQVATYPTERMVTLEQLNELLEQAHRQEIAPAELLDPL
LMPMDSPVENYPEVNLLPVVAGYVKQGQPVQVAGAPASGMVRITEGEERKFIGVGDIADDGRVAPRRLVVEYFD
>Mature_313_residues
SRPRRRGRDIHGVLLLDKPQGLSSNDALQKVKRLYNANRAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVIAR
LGQRTDTSDADGQIVQERPVSFTQAQLDAALDSFRGDIKQVPSMYSALKYQGKKLYEYARQGIEVPREARSITVYELQFI
RWEGDELELEIHCSKGTYIRTITDDLGELLGCGAHVIYLRRLQVATYPTERMVTLEQLNELLEQAHRQEIAPAELLDPLL
MPMDSPVENYPEVNLLPVVAGYVKQGQPVQVAGAPASGMVRITEGEERKFIGVGDIADDGRVAPRRLVVEYFD

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=251, Percent_Identity=31.4741035856574, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI215599015, Length=173, Percent_Identity=27.1676300578035, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI4503337, Length=173, Percent_Identity=27.1676300578035, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI2367200, Length=312, Percent_Identity=81.7307692307692, Blast_Score=523, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17553978, Length=210, Percent_Identity=29.0476190476191, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=142, Percent_Identity=37.3239436619718, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=280, Percent_Identity=26.4285714285714, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364189, Length=270, Percent_Identity=25.1851851851852, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364187, Length=270, Percent_Identity=25.1851851851852, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364185, Length=270, Percent_Identity=25.1851851851852, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364183, Length=270, Percent_Identity=25.1851851851852, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62471759, Length=270, Percent_Identity=25.1851851851852, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17975520, Length=270, Percent_Identity=25.1851851851852, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_SERP5 (A8G909)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476727.1
- ProteinModelPortal:   A8G909
- SMR:   A8G909
- STRING:   A8G909
- GeneID:   5606537
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0491
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK05033
- BioCyc:   SPRO399741:SPRO_0491-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 35184; Mature: 35052

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: ACT_SITE 48-48 BINDING 43-43 BINDING 76-76 BINDING 179-179 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPRRRGRDIHGVLLLDKPQGLSSNDALQKVKRLYNANRAGHTGALDPLATGMLPICLG
CCCCCHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDSDKRYRVIARLGQRTDTSDADGQIVQERPVSFTQAQLDAALDSFRGDIK
CHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHH
QVPSMYSALKYQGKKLYEYARQGIEVPREARSITVYELQFIRWEGDELELEIHCSKGTYI
HHHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCEEEEEEEEEEECCCEEEEEEEECCCCEE
RTITDDLGELLGCGAHVIYLRRLQVATYPTERMVTLEQLNELLEQAHRQEIAPAELLDPL
EEHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LMPMDSPVENYPEVNLLPVVAGYVKQGQPVQVAGAPASGMVRITEGEERKFIGVGDIADD
HCCCCCCHHCCCCCEEHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCC
GRVAPRRLVVEYFD
CCCCCHHHEEEECC
>Mature Secondary Structure 
SRPRRRGRDIHGVLLLDKPQGLSSNDALQKVKRLYNANRAGHTGALDPLATGMLPICLG
CCCCHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDSDKRYRVIARLGQRTDTSDADGQIVQERPVSFTQAQLDAALDSFRGDIK
CHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHH
QVPSMYSALKYQGKKLYEYARQGIEVPREARSITVYELQFIRWEGDELELEIHCSKGTYI
HHHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCEEEEEEEEEEECCCEEEEEEEECCCCEE
RTITDDLGELLGCGAHVIYLRRLQVATYPTERMVTLEQLNELLEQAHRQEIAPAELLDPL
EEHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LMPMDSPVENYPEVNLLPVVAGYVKQGQPVQVAGAPASGMVRITEGEERKFIGVGDIADD
HCCCCCCHHCCCCCEEHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCC
GRVAPRRLVVEYFD
CCCCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA