Click here to switch to the map view.

The map label for this gene is rbfA

Identifier: 157368737

GI number: 157368737

Start: 542232

End: 542642

Strand: Direct

Name: rbfA

Synonym: Spro_0490

Alternate gene names: 157368737

Gene position: 542232-542642 (Clockwise)

Preceding gene: 157368736

Following gene: 157368738

Centisome position: 9.95

GC content: 53.53

Gene sequence:

>411_bases
ATGGCAAAAGAATTCAGCCGCGGTCAGCGCGTGGCACAAGAGATGCAAAAAGAGATTGCTATCATTCTGCAGCGTGAAGT
CAAAGATCCGCGTGTCGGCATGGCTACCGTTTCTGGTGTAGAAGTTTCTCGTGATTTGGCGTATGCCAAAGTTTACGTCA
CCTTCCTGAACGTATTGACCGAAAACGCCGATCCGAATCTGGTGCCTAACGGCATCAAAGCTCTGGAAGACGCTTCCGGC
TACATCCGCACGCTGCTGGGTAAAGCCATGCGCCTGCGCGTAGTACCTGAGCTGACCTTCGCTTACGACAACTCCCTGGT
TGAAGGCATGCGCATGTCCAACCTGGTGACCAACGTAGTGAAGAATGACGCCGAACGCCGTTCCGCATCAGGCGATGACG
AGGAGGCTTAA

Upstream 100 bases:

>100_bases
AAATCATCGAGATCCAACGCACCATCGCTTAACGCTAGCACATCTGCTTAAGTCTGTTCTGGGGGGCCTTGTGCCCCCCG
ATTTGTCTGGAGAATCCATA

Downstream 100 bases:

>100_bases
TGAGTCGCCCTCGTCGCCGCGGCCGTGATATCCACGGTGTCCTGTTGCTGGACAAACCGCAGGGCCTGTCATCCAACGAC
GCCCTGCAAAAAGTAAAGCG

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MAKEFSRGQRVAQEMQKEIAIILQREVKDPRVGMATVSGVEVSRDLAYAKVYVTFLNVLTENADPNLVPNGIKALEDASG
YIRTLLGKAMRLRVVPELTFAYDNSLVEGMRMSNLVTNVVKNDAERRSASGDDEEA

Sequences:

>Translated_136_residues
MAKEFSRGQRVAQEMQKEIAIILQREVKDPRVGMATVSGVEVSRDLAYAKVYVTFLNVLTENADPNLVPNGIKALEDASG
YIRTLLGKAMRLRVVPELTFAYDNSLVEGMRMSNLVTNVVKNDAERRSASGDDEEA
>Mature_135_residues
AKEFSRGQRVAQEMQKEIAIILQREVKDPRVGMATVSGVEVSRDLAYAKVYVTFLNVLTENADPNLVPNGIKALEDASGY
IRTLLGKAMRLRVVPELTFAYDNSLVEGMRMSNLVTNVVKNDAERRSASGDDEEA

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family

Homologues:

Organism=Escherichia coli, GI1789558, Length=136, Percent_Identity=75, Blast_Score=199, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RBFA_SERP5 (A8G908)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476726.1
- ProteinModelPortal:   A8G908
- SMR:   A8G908
- STRING:   A8G908
- GeneID:   5606536
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0490
- eggNOG:   COG0858
- HOGENOM:   HBG751055
- OMA:   SIEYGNR
- ProtClustDB:   PRK00521
- BioCyc:   SPRO399741:SPRO_0490-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00003
- InterPro:   IPR015946
- InterPro:   IPR000238
- InterPro:   IPR020053
- Gene3D:   G3DSA:3.30.300.20
- ProDom:   PD007327
- TIGRFAMs:   TIGR00082

Pfam domain/function: PF02033 RBFA; SSF89919 Rib_bd_factA

EC number: NA

Molecular weight: Translated: 15063; Mature: 14932

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEFSRGQRVAQEMQKEIAIILQREVKDPRVGMATVSGVEVSRDLAYAKVYVTFLNVLT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHH
ENADPNLVPNGIKALEDASGYIRTLLGKAMRLRVVPELTFAYDNSLVEGMRMSNLVTNVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNDAERRSASGDDEEA
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AKEFSRGQRVAQEMQKEIAIILQREVKDPRVGMATVSGVEVSRDLAYAKVYVTFLNVLT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHH
ENADPNLVPNGIKALEDASGYIRTLLGKAMRLRVVPELTFAYDNSLVEGMRMSNLVTNVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNDAERRSASGDDEEA
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA