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The map label for this gene is purH [H]

Identifier: 157368542

GI number: 157368542

Start: 334269

End: 335858

Strand: Reverse

Name: purH [H]

Synonym: Spro_0293

Alternate gene names: 157368542

Gene position: 335858-334269 (Counterclockwise)

Preceding gene: 157368544

Following gene: 157368541

Centisome position: 6.16

GC content: 58.99

Gene sequence:

>1590_bases
ATGCAACAACCTCGTCCAATCCGCCGGGCCCTGCTCAGCGTCTCTGACAAAGCCGGTATCGTTGAATTCGCCGAAGCGCT
GTCCCAGCGTGGCGTTGAACTGCTCTCCACCGGTGGCACCGCCCGCCTGCTGGCAGATGCCGGCCTGCCTGTTACCGAAG
TTTCCGACTACACCGGCTTCCCGGAAATGATGGACGGACGAGTAAAGACCCTGCACCCCAAAGTACACGGCGGTATTCTC
GGCCGCCGCGGCCAGGACGACGCCATCATGGGTCAGCATGACATCAAGCCGATCGACATGGTGGTGGTAAACCTCTATCC
GTTCGCCCAGACCGTGGCGCGCCCGAACTGCTCACTGGAAGACGCGGTCGAGAACATCGACATCGGCGGCCCAACCATGG
TGCGTTCCGCGGCCAAGAACCACAAAGACGTCGCCATCGTGGTAAAGAGCAGCGACTACGCCGCTATTATTACCGAGATG
GATAACAACGACGGTTCACTGCAATACACCACCCGTTTCGATCTGGCCATCAAAGCCTTCGAGCACACCGCCGCTTACGA
CAGCATGATCGCCAACTACTTCGGCGCGCTGGTTCCGGCCTACCACGGCGATACCGAACAACCTGCCGGTCGTTTCCCTC
GCACCCTGAACCTCAACTATATAAAGAAGCAGGATATGCGCTACGGTGAGAACAGCCACCAGCAAGCAGCCTTCTATATA
GAAGAGAACGTTCAGGAAGCCTCTGTCGCCACCGCGGAACAACTGCAAGGCAAAGCGCTGTCCTACAACAACATCGCCGA
CACCGACGCCGCACTGGAATGTGTGAAGGAATTCGCCGAGCCGGCCTGCGTGATCGTCAAGCACGCCAACCCATGCGGTG
TGGCGATCGGCGATGATATTCTGTCTGCCTATGAGCGCGCCTATCAAACCGACCCGACCTCTGCTTTCGGCGGCATCATC
GCCTTTAACCGCGAACTGGACGCCGCTACCGCACAGGCCATTATCAGCCGTCAGTTTGTGGAAGTGATTATCGCGCCGAG
CATCAGTCAGGAAGCTCGCTCCCTGTTGGCAGCCAAACAGAACGTGCGCGTACTGGCCTGCGGCCAATGGCAGCAACGTA
TTGCCGCTCTCGACTTCAAACGTGTCAACGGTGGCCTGCTGGTGCAAGACCGCGATCTGGGTATGGTGAGCGAAGGCGAC
CTGCGCGTGGTATCTGAACGTCAGCCGACCGCGCAGGAACTGCGTGATGCGCTGTTCTGTTGGAAAGTCGCCAAGTTCGT
GAAGTCCAACGCTATCGTCTATGCACGTGACAACATGACCATCGGCATAGGCGCCGGGCAAATGAGCCGCGTTTACTCTG
CCAAGATCGCCGGGATCAAAGCCGCGGACGAAGGCCTGGAAGTCAAAGGCTCCGCCATGGCGTCTGACGCTTTCTTCCCG
TTCCGTGATGGCATCGATGCCGCCGCAGCGGTGGGCATCAGCTGCGTGATCCAGCCAGGCGGTTCGATCCGCGATGATGA
AGTGATTGCCGCCGCCAATGAGCACGGCATCGCAATGATCTTCACCGACATGCGCCACTTCCGCCATTAA

Upstream 100 bases:

>100_bases
TACCCCTTTATAGAGAGAAAAACCTTTCCTTATAAAGAGTAGAAGGTGGAAATTCACGTAACGCTCTTTTATTGCGACTC
CAAAGCCAAGGGATAAAACC

Downstream 100 bases:

>100_bases
TCCCTTTTGCCGCGTGCGCTACGGCGCACGCTCTGCGTGGAGCACCTGATGAATATTTTAATTATCGGCAACGGCGGGCG
TGAACACGCGCTGGCCTGGA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MQQPRPIRRALLSVSDKAGIVEFAEALSQRGVELLSTGGTARLLADAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAIMGQHDIKPIDMVVVNLYPFAQTVARPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYAAIITEM
DNNDGSLQYTTRFDLAIKAFEHTAAYDSMIANYFGALVPAYHGDTEQPAGRFPRTLNLNYIKKQDMRYGENSHQQAAFYI
EENVQEASVATAEQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGDDILSAYERAYQTDPTSAFGGII
AFNRELDAATAQAIISRQFVEVIIAPSISQEARSLLAAKQNVRVLACGQWQQRIAALDFKRVNGGLLVQDRDLGMVSEGD
LRVVSERQPTAQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
FRDGIDAAAAVGISCVIQPGGSIRDDEVIAAANEHGIAMIFTDMRHFRH

Sequences:

>Translated_529_residues
MQQPRPIRRALLSVSDKAGIVEFAEALSQRGVELLSTGGTARLLADAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAIMGQHDIKPIDMVVVNLYPFAQTVARPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYAAIITEM
DNNDGSLQYTTRFDLAIKAFEHTAAYDSMIANYFGALVPAYHGDTEQPAGRFPRTLNLNYIKKQDMRYGENSHQQAAFYI
EENVQEASVATAEQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGDDILSAYERAYQTDPTSAFGGII
AFNRELDAATAQAIISRQFVEVIIAPSISQEARSLLAAKQNVRVLACGQWQQRIAALDFKRVNGGLLVQDRDLGMVSEGD
LRVVSERQPTAQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
FRDGIDAAAAVGISCVIQPGGSIRDDEVIAAANEHGIAMIFTDMRHFRH
>Mature_529_residues
MQQPRPIRRALLSVSDKAGIVEFAEALSQRGVELLSTGGTARLLADAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAIMGQHDIKPIDMVVVNLYPFAQTVARPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYAAIITEM
DNNDGSLQYTTRFDLAIKAFEHTAAYDSMIANYFGALVPAYHGDTEQPAGRFPRTLNLNYIKKQDMRYGENSHQQAAFYI
EENVQEASVATAEQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGDDILSAYERAYQTDPTSAFGGII
AFNRELDAATAQAIISRQFVEVIIAPSISQEARSLLAAKQNVRVLACGQWQQRIAALDFKRVNGGLLVQDRDLGMVSEGD
LRVVSERQPTAQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
FRDGIDAAAAVGISCVIQPGGSIRDDEVIAAANEHGIAMIFTDMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=488, Percent_Identity=38.9344262295082, Blast_Score=280, Evalue=3e-75,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=87.7126654064272, Blast_Score=965, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=33.5504885993485, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71985574, Length=352, Percent_Identity=27.8409090909091, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=115, Percent_Identity=37.3913043478261, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=485, Percent_Identity=35.4639175257732, Blast_Score=261, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6323056, Length=497, Percent_Identity=34.20523138833, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24649832, Length=611, Percent_Identity=34.860883797054, Blast_Score=301, Evalue=1e-81,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57336; Mature: 57336

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQPRPIRRALLSVSDKAGIVEFAEALSQRGVELLSTGGTARLLADAGLPVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCEEEEECCCCCHHCCCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDAIMGQHDIKPIDMVVVNLYPFAQTVARPNCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYAAIITEMDNNDGSLQYTTRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEEEEEEHHH
EHTAAYDSMIANYFGALVPAYHGDTEQPAGRFPRTLNLNYIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCEEEEE
EENVQEASVATAEQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGDDI
ECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECHHH
LSAYERAYQTDPTSAFGGIIAFNRELDAATAQAIISRQFVEVIIAPSISQEARSLLAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
NVRVLACGQWQQRIAALDFKRVNGGLLVQDRDLGMVSEGDLRVVSERQPTAQELRDALFC
CCEEEEECCHHHHHHHHHHEECCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
HHHHHHHHCCCEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCEEECCCCCC
FRDGIDAAAAVGISCVIQPGGSIRDDEVIAAANEHGIAMIFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MQQPRPIRRALLSVSDKAGIVEFAEALSQRGVELLSTGGTARLLADAGLPVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCEEEEECCCCCHHCCCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDAIMGQHDIKPIDMVVVNLYPFAQTVARPNCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYAAIITEMDNNDGSLQYTTRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEEEEEEHHH
EHTAAYDSMIANYFGALVPAYHGDTEQPAGRFPRTLNLNYIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCEEEEE
EENVQEASVATAEQLQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGDDI
ECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECHHH
LSAYERAYQTDPTSAFGGIIAFNRELDAATAQAIISRQFVEVIIAPSISQEARSLLAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
NVRVLACGQWQQRIAALDFKRVNGGLLVQDRDLGMVSEGDLRVVSERQPTAQELRDALFC
CCEEEEECCHHHHHHHHHHEECCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
HHHHHHHHCCCEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCEEECCCCCC
FRDGIDAAAAVGISCVIQPGGSIRDDEVIAAANEHGIAMIFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA