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The map label for this gene is rsmD [H]

Identifier: 157368469

GI number: 157368469

Start: 247712

End: 248332

Strand: Reverse

Name: rsmD [H]

Synonym: Spro_0220

Alternate gene names: 157368469

Gene position: 248332-247712 (Counterclockwise)

Preceding gene: 157368474

Following gene: 157368468

Centisome position: 4.56

GC content: 60.55

Gene sequence:

>621_bases
ATGACAAGAATATCGCCACGGGCGTCGGCAAAAAAACCGCCTCAGGCTGCGGCCGGGCAGATCCGTATTATCGGCGGCCA
ATGGCGCGGACGGAAACTGCCGGTGCCAAATAGCCCTGGGCTACGTCCAACCACCGATCGCGTCCGTGAAACCCTGTTCA
ACTGGCTGGCCCCGGTGATCCAGGGCGCACGCTGCCTGGACTGTTTCGCCGGTAGCGGCGCACTCGGCCTGGAAGCGCTG
TCCCGCTATGCCGGCAGCGTTACGCTGTTGGAGTTTGAACGCCCGGTGGCGCAGCAGTTGGAAAAGAATCTGGCGCTGCT
GCAAGGCAAAGGCCATGTGGTCAACACCAACGCGCTGAGCTGGCTGGCGAACAATGCCCAACCGTTTGACGTCGTGTTTC
TCGATCCGCCTTTTCGCAAAGGGCTGCTGGCGGAAACCGTCACGCTGCTGGAGCAGCAGGGCTGGCTGGCCGATGAGGCC
TGGATTTACGTAGAGGCCGAAGCCGAAAGCGCCGCCACCGACGTTCCGGCCAACTGGCAGCTACACCGTGAAAAAGTCGC
CGGTCAGGTGGCTTACCGTCTTTATATTCGCTCTCAAGAGAAAACCGACAATGCTGATTAA

Upstream 100 bases:

>100_bases
GTTTTACCGTTTCCCCCGGCGCGTCTCACCGGTAGAATAGAGGCAACTTTTCGCTAACTTGTGCGGCCTTCACCGCCCCA
AACAGAAATAAACCAATCCT

Downstream 100 bases:

>100_bases
TCTGGGTCGCCTGCTGATGCTGTGCGTATGGGGCTTTCTGCTCTCCAATCTGTTTCACCCTTTTCCCAAGCCGCTGAAGT
ACTTCATCGACGTGGCGCTG

Product: 16S rRNA m(2)G966-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTRISPRASAKKPPQAAAGQIRIIGGQWRGRKLPVPNSPGLRPTTDRVRETLFNWLAPVIQGARCLDCFAGSGALGLEAL
SRYAGSVTLLEFERPVAQQLEKNLALLQGKGHVVNTNALSWLANNAQPFDVVFLDPPFRKGLLAETVTLLEQQGWLADEA
WIYVEAEAESAATDVPANWQLHREKVAGQVAYRLYIRSQEKTDNAD

Sequences:

>Translated_206_residues
MTRISPRASAKKPPQAAAGQIRIIGGQWRGRKLPVPNSPGLRPTTDRVRETLFNWLAPVIQGARCLDCFAGSGALGLEAL
SRYAGSVTLLEFERPVAQQLEKNLALLQGKGHVVNTNALSWLANNAQPFDVVFLDPPFRKGLLAETVTLLEQQGWLADEA
WIYVEAEAESAATDVPANWQLHREKVAGQVAYRLYIRSQEKTDNAD
>Mature_205_residues
TRISPRASAKKPPQAAAGQIRIIGGQWRGRKLPVPNSPGLRPTTDRVRETLFNWLAPVIQGARCLDCFAGSGALGLEALS
RYAGSVTLLEFERPVAQQLEKNLALLQGKGHVVNTNALSWLANNAQPFDVVFLDPPFRKGLLAETVTLLEQQGWLADEAW
IYVEAEAESAATDVPANWQLHREKVAGQVAYRLYIRSQEKTDNAD

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=197, Percent_Identity=70.5583756345178, Blast_Score=288, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22671; Mature: 22540

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRISPRASAKKPPQAAAGQIRIIGGQWRGRKLPVPNSPGLRPTTDRVRETLFNWLAPVI
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QGARCLDCFAGSGALGLEALSRYAGSVTLLEFERPVAQQLEKNLALLQGKGHVVNTNALS
CCCHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHEECCCCEEEECHHHH
WLANNAQPFDVVFLDPPFRKGLLAETVTLLEQQGWLADEAWIYVEAEAESAATDVPANWQ
HHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCH
LHREKVAGQVAYRLYIRSQEKTDNAD
HHHHHHHHHHEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TRISPRASAKKPPQAAAGQIRIIGGQWRGRKLPVPNSPGLRPTTDRVRETLFNWLAPVI
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QGARCLDCFAGSGALGLEALSRYAGSVTLLEFERPVAQQLEKNLALLQGKGHVVNTNALS
CCCHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHEECCCCEEEECHHHH
WLANNAQPFDVVFLDPPFRKGLLAETVTLLEQQGWLADEAWIYVEAEAESAATDVPANWQ
HHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCH
LHREKVAGQVAYRLYIRSQEKTDNAD
HHHHHHHHHHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]