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The map label for this gene is hemC

Identifier: 157368427

GI number: 157368427

Start: 201395

End: 202336

Strand: Reverse

Name: hemC

Synonym: Spro_0178

Alternate gene names: 157368427

Gene position: 202336-201395 (Counterclockwise)

Preceding gene: 157368429

Following gene: 157368426

Centisome position: 3.71

GC content: 60.3

Gene sequence:

>942_bases
ATGTTAGACAAAATTATTCGAATTGCCACCCGACAAAGCCCACTCGCTCTCTGGCAGGCACATTATGTGCAGCAGCGCCT
GATGGCCAACCACCCTGGCCTGCAGGTTGAACTGGTGCCAATGGTCACTAAGGGCGACATTATTCTGGATACGCCACTGG
CGAAGGTCGGTGGTAAAGGGCTGTTTGTTAAGGAACTTGAGCTGGCGTTACTCGATGGCCGTGCCGATATCGCCGTCCAT
TCGATGAAAGACGTCCCGGTAGATTTCCCCGCCGGCCTGGGTTTGGTGACCATTTGCGAGCGCGACGATCCACGCGACGC
ATTTGTATCCAATCGTTTCACCTCGCTCGATCAACTGCCGCAGGGCAGCGTGGTTGGCACCTCCAGCCTGCGCCGCCAGT
GCCAGTTACGTGAGCGTCGTCCGGACCTGATCGTGCGCGATCTGCGCGGCAACGTTGGCACCCGTTTGTCAAAACTGGAC
AACGGTGATTACGACGCCATTATTCTGGCGGTCGCCGGGCTCAAGCGCCTGGGTCTGGAACAGCGCATCCGCAGCCCGCT
TAGCCCGGAAGAGTGCTTGCCGGCGGTCGGTCAGGGTGCTGTGGGCATTGAGTGCCGCCTCGACGATGAAACCACCCGTG
CGCTACTGGCCCCGCTCAATCATGCCGTCACCGAAACCCGCGTTCGTGCCGAACGTGCGATGAATACCCGGTTGGAAGGC
GGTTGTCAGGTGCCGATCGGCAGCTATGCCGAACTCGATGGCGACAGCCTGTGGCTGCGTGCATTGGTAGGCTCCCCGGA
CGGCAGTCAGATGGTGCGCGGCGAACGTCGCGGCCCGGCAGCGCTGGCGGAACAAATGGGCATTGAGCTGGCGGAAGAGT
TGCTGGCGCAGGGCGCACGCGAAATTCTACAGGATGTTTATCAGGGAAATCCTCCGGCATGA

Upstream 100 bases:

>100_bases
CGTTGATTCCGTTGTCCTTTCTTCGCCTGCGGCTTCTTGCACTGACAGTTTTCACACTCAGTGGTAGGATGAACGGCGAA
TTATAAAAACGGTAACAAGC

Downstream 100 bases:

>100_bases
CGATTCTGGTAACCCGCCCTTCTCCTTCGGGAGAGCAATTGGTAAGCCGACTGCGTGCGCTCGGCCGGGTTGCCTATCAT
GCCCCGCTGATCGATTTCGC

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLDKIIRIATRQSPLALWQAHYVQQRLMANHPGLQVELVPMVTKGDIILDTPLAKVGGKGLFVKELELALLDGRADIAVH
SMKDVPVDFPAGLGLVTICERDDPRDAFVSNRFTSLDQLPQGSVVGTSSLRRQCQLRERRPDLIVRDLRGNVGTRLSKLD
NGDYDAIILAVAGLKRLGLEQRIRSPLSPEECLPAVGQGAVGIECRLDDETTRALLAPLNHAVTETRVRAERAMNTRLEG
GCQVPIGSYAELDGDSLWLRALVGSPDGSQMVRGERRGPAALAEQMGIELAEELLAQGAREILQDVYQGNPPA

Sequences:

>Translated_313_residues
MLDKIIRIATRQSPLALWQAHYVQQRLMANHPGLQVELVPMVTKGDIILDTPLAKVGGKGLFVKELELALLDGRADIAVH
SMKDVPVDFPAGLGLVTICERDDPRDAFVSNRFTSLDQLPQGSVVGTSSLRRQCQLRERRPDLIVRDLRGNVGTRLSKLD
NGDYDAIILAVAGLKRLGLEQRIRSPLSPEECLPAVGQGAVGIECRLDDETTRALLAPLNHAVTETRVRAERAMNTRLEG
GCQVPIGSYAELDGDSLWLRALVGSPDGSQMVRGERRGPAALAEQMGIELAEELLAQGAREILQDVYQGNPPA
>Mature_313_residues
MLDKIIRIATRQSPLALWQAHYVQQRLMANHPGLQVELVPMVTKGDIILDTPLAKVGGKGLFVKELELALLDGRADIAVH
SMKDVPVDFPAGLGLVTICERDDPRDAFVSNRFTSLDQLPQGSVVGTSSLRRQCQLRERRPDLIVRDLRGNVGTRLSKLD
NGDYDAIILAVAGLKRLGLEQRIRSPLSPEECLPAVGQGAVGIECRLDDETTRALLAPLNHAVTETRVRAERAMNTRLEG
GCQVPIGSYAELDGDSLWLRALVGSPDGSQMVRGERRGPAALAEQMGIELAEELLAQGAREILQDVYQGNPPA

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=340, Percent_Identity=42.6470588235294, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI66933009, Length=340, Percent_Identity=42.6470588235294, Blast_Score=241, Evalue=5e-64,
Organism=Escherichia coli, GI48994974, Length=313, Percent_Identity=80.5111821086262, Blast_Score=507, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6319996, Length=319, Percent_Identity=37.9310344827586, Blast_Score=183, Evalue=3e-47,
Organism=Drosophila melanogaster, GI20130425, Length=260, Percent_Identity=43.8461538461538, Blast_Score=206, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_SERP5 (A8G848)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476416.1
- ProteinModelPortal:   A8G848
- SMR:   A8G848
- STRING:   A8G848
- GeneID:   5605368
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0178
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   SPRO399741:SPRO_0178-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34100; Mature: 34100

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKIIRIATRQSPLALWQAHYVQQRLMANHPGLQVELVPMVTKGDIILDTPLAKVGGKG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEECCHHHHCCCC
LFVKELELALLDGRADIAVHSMKDVPVDFPAGLGLVTICERDDPRDAFVSNRFTSLDQLP
EEHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
QGSVVGTSSLRRQCQLRERRPDLIVRDLRGNVGTRLSKLDNGDYDAIILAVAGLKRLGLE
CCCEECHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
QRIRSPLSPEECLPAVGQGAVGIECRLDDETTRALLAPLNHAVTETRVRAERAMNTRLEG
HHHCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GCQVPIGSYAELDGDSLWLRALVGSPDGSQMVRGERRGPAALAEQMGIELAEELLAQGAR
CCCCCCCCCCCCCCCCEEEEHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EILQDVYQGNPPA
HHHHHHHCCCCCC
>Mature Secondary Structure
MLDKIIRIATRQSPLALWQAHYVQQRLMANHPGLQVELVPMVTKGDIILDTPLAKVGGKG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEECCHHHHCCCC
LFVKELELALLDGRADIAVHSMKDVPVDFPAGLGLVTICERDDPRDAFVSNRFTSLDQLP
EEHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
QGSVVGTSSLRRQCQLRERRPDLIVRDLRGNVGTRLSKLDNGDYDAIILAVAGLKRLGLE
CCCEECHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
QRIRSPLSPEECLPAVGQGAVGIECRLDDETTRALLAPLNHAVTETRVRAERAMNTRLEG
HHHCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GCQVPIGSYAELDGDSLWLRALVGSPDGSQMVRGERRGPAALAEQMGIELAEELLAQGAR
CCCCCCCCCCCCCCCCEEEEHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EILQDVYQGNPPA
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA