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The map label for this gene is fdoH [H]

Identifier: 157368334

GI number: 157368334

Start: 90073

End: 90972

Strand: Direct

Name: fdoH [H]

Synonym: Spro_0085

Alternate gene names: 157368334

Gene position: 90073-90972 (Clockwise)

Preceding gene: 157368333

Following gene: 157368335

Centisome position: 1.65

GC content: 61.11

Gene sequence:

>900_bases
ATGGCAATGCAATCTCAGGACATCATTCGTAAATCCGCCACCAATGGTTTCACGCCGGCGCCGCGCGCCCGTGACCACCA
GGAGGAAGTGGCCAAACTGATCGATGTCACCACCTGTATCGGCTGCAAGGCCTGTCAGGTGGCCTGTTCCGAATGGAACG
ACATCCGCGATGAAATCGGCAGCAACGTCGGGGTGTACGATAACCCCGCCGATCTGACCGCCAAGTCGTGGACGGTGATG
CGCTTTTCCGAGGTGGAGGAAAACGGCAAGCTGGAGTGGCTGATCCGCAAGGACGGCTGCATGCACTGCGCCGATCCGGG
CTGCCTGAAGGCCTGCCCGTCGGAAGGAGCCATCATCCAGTACGCCAACGGCATCGTCGATTTCCAGTCCGAACACTGCA
TCGGCTGCGGTTACTGCATCGCCGGTTGCCCGTTCGATGTGCCGCGCATGAACAAGGACGACAACCGGGTGTACAAGTGC
ACCCTGTGCGTCGATCGGGTTGATGTCGGCCAGGAACCGGCCTGTGTGAAGACCTGTCCGACCGGGGCCATCCATTTCGG
CACCAAGGACGCCATGAAGGAAATCGCCGCCGAGCGCGTCAGCGAGCTGAACACCCGTGGTTATCAGAACGCCGGTCTGT
ACGATCCGGCGGGCGTGGGCGGCACCCACGTGATGTATGTGCTGCACCATGCCGACAAGCCGCAGCTGTATCACGGTTTG
CCGGATAACCCGACCATCAGCCCGGCAATCACGTTCTGGAAAGGCGTGTGGAAACCCCTGGCGGCCATCGGCTTTGCCGC
CACCTTTGCAGCGAGCGTGTTCCACTATGTCGGCGTCGGTCCTAACCGCGTCGAAGAGGAAGACCACGACGAGCTGCACG
ATGAGGAGACGCGCAAATGA

Upstream 100 bases:

>100_bases
TGCCAACACGCTGACGCCGTTTGTCGGTGATGCCAATACGCAAACGCCAGAGTTCAAGGCGTTCCTGGTCAACGTGGAAA
AGGTGTAACGGAGACGAATT

Downstream 100 bases:

>100_bases
AGAAGGAAAAGCGTATTCAGCGCTACAGCGCGCCGGAGCGGATTAACCACTGGATTGTGGCGTTCTGCTTTGTGTTCGCC
GCCATTAGCGGGCTGGGGTT

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MAMQSQDIIRKSATNGFTPAPRARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEIGSNVGVYDNPADLTAKSWTVM
RFSEVEENGKLEWLIRKDGCMHCADPGCLKACPSEGAIIQYANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVYKC
TLCVDRVDVGQEPACVKTCPTGAIHFGTKDAMKEIAAERVSELNTRGYQNAGLYDPAGVGGTHVMYVLHHADKPQLYHGL
PDNPTISPAITFWKGVWKPLAAIGFAATFAASVFHYVGVGPNRVEEEDHDELHDEETRK

Sequences:

>Translated_299_residues
MAMQSQDIIRKSATNGFTPAPRARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEIGSNVGVYDNPADLTAKSWTVM
RFSEVEENGKLEWLIRKDGCMHCADPGCLKACPSEGAIIQYANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVYKC
TLCVDRVDVGQEPACVKTCPTGAIHFGTKDAMKEIAAERVSELNTRGYQNAGLYDPAGVGGTHVMYVLHHADKPQLYHGL
PDNPTISPAITFWKGVWKPLAAIGFAATFAASVFHYVGVGPNRVEEEDHDELHDEETRK
>Mature_298_residues
AMQSQDIIRKSATNGFTPAPRARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEIGSNVGVYDNPADLTAKSWTVMR
FSEVEENGKLEWLIRKDGCMHCADPGCLKACPSEGAIIQYANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVYKCT
LCVDRVDVGQEPACVKTCPTGAIHFGTKDAMKEIAAERVSELNTRGYQNAGLYDPAGVGGTHVMYVLHHADKPQLYHGLP
DNPTISPAITFWKGVWKPLAAIGFAATFAASVFHYVGVGPNRVEEEDHDELHDEETRK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=301, Percent_Identity=83.7209302325581, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1787749, Length=294, Percent_Identity=75.1700680272109, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1789370, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787122, Length=166, Percent_Identity=33.1325301204819, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787872, Length=166, Percent_Identity=31.9277108433735, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI2367345, Length=178, Percent_Identity=29.7752808988764, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI226510944, Length=169, Percent_Identity=28.9940828402367, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082179, Length=172, Percent_Identity=27.906976744186, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787740, Length=125, Percent_Identity=32, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787478, Length=100, Percent_Identity=34, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788811, Length=174, Percent_Identity=24.1379310344828, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787963, Length=148, Percent_Identity=24.3243243243243, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 32829; Mature: 32698

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMQSQDIIRKSATNGFTPAPRARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEIG
CCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
SNVGVYDNPADLTAKSWTVMRFSEVEENGKLEWLIRKDGCMHCADPGCLKACPSEGAIIQ
CCCCCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEE
YANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVYKCTLCVDRVDVGQEPACVKTCP
ECCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCCCEEECC
TGAIHFGTKDAMKEIAAERVSELNTRGYQNAGLYDPAGVGGTHVMYVLHHADKPQLYHGL
CCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEECCC
PDNPTISPAITFWKGVWKPLAAIGFAATFAASVFHYVGVGPNRVEEEDHDELHDEETRK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCHHHCCC
>Mature Secondary Structure 
AMQSQDIIRKSATNGFTPAPRARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEIG
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
SNVGVYDNPADLTAKSWTVMRFSEVEENGKLEWLIRKDGCMHCADPGCLKACPSEGAIIQ
CCCCCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEE
YANGIVDFQSEHCIGCGYCIAGCPFDVPRMNKDDNRVYKCTLCVDRVDVGQEPACVKTCP
ECCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCCCEEECC
TGAIHFGTKDAMKEIAAERVSELNTRGYQNAGLYDPAGVGGTHVMYVLHHADKPQLYHGL
CCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEECCC
PDNPTISPAITFWKGVWKPLAAIGFAATFAASVFHYVGVGPNRVEEEDHDELHDEETRK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]