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The map label for this gene is ghrB

Identifier: 157368306

GI number: 157368306

Start: 56756

End: 57733

Strand: Reverse

Name: ghrB

Synonym: Spro_0057

Alternate gene names: 157368306

Gene position: 57733-56756 (Counterclockwise)

Preceding gene: 157368307

Following gene: 157368303

Centisome position: 1.06

GC content: 57.87

Gene sequence:

>978_bases
ATGAAGCCTTCTATCGTTTTATACAAAAATATCCCCGCCGACCTGCGTGAACGACTGGAACAGCACTTCACCGTGCACGC
CTTCGACGGCCTGACCCCGGATAACCACGATGAGCTGCGCCAGGCGTTGCAACAGGCGGAAGGGATCATCGGTTCCGGCG
GTAAAATTGATGAGGCTTTCCTGCAGCAGACGCCAAAACTGCGTGCGGCCTCAACCATTTCCGTCGGTTACGACAATTTC
GACGTCGACGCGCTTAATGCTCATAACGTGCTGCTGATGCATACCCCTACCGTGCTGACCGAAACCGTCGCCGATACCAT
CATGAGTCTGGTGCTGGCGACAGCGCGCCGGGTGGTGGAAGTGGCCGAACGGGTAAAGGCCGGCGAATGGCAGGGCAGCA
TTGGAGCCGACTGGTTTGGCGTCGATGTACATCATAAAACCATCGGCATCCTCGGGATGGGCCGAATTGGCCTGGCGCTG
GCGCAACGTGCGCACTTCGGCTTCGGCATGCCGGTGTTGTATAACGCCCGCCGCACGCACGAAGAAGCCGAGCAACGTTT
TAACGCCCGCCGCTGTGACCTGGATACGCTATTGGCTGAATCCGATTTCATCTGCATTACGCTGCCGCTGACGGATGAAA
CCTTCCATATGATCAGCCGTGACCAACTGGCGAAAATGAAAAAGAGCGGCATCCTGATCAACGCCGGTCGTGGCCCGGTG
GTGGACGAAGCTGCGCTGATAGAAGCGCTGCAGAACGGGACCATCCATGCCGCCGGGCTGGACGTGTTCGAAAAAGAGCC
GCTGCCGGTGTCTTCTCCGCTGCTGACGCTGCCAAACGTAGTGGCACTGCCGCATATCGGTTCTGCGACCCATGAGACCC
GCTATGGCATGGCCGAATGTGCCGTCGACAACCTGATTGCCGCCCTGACCGGCACGGTCAAAGAAAACTGCGTTAATCCG
CAACTGCTGAAAAAGTAA

Upstream 100 bases:

>100_bases
CCTGAATGACCGGGGTGACGGGCGCTGCGCCCTTCACCCTTTTTGCTTTATGTTATGTTGGATCCCCAACGTCCTAATCG
TTAGCTTGCTGGAGTTCGTG

Downstream 100 bases:

>100_bases
CTTTCATGGATGCCAGTGAAATAAAAACTGGCATCCATTCAAAATTACCAAGATTTTTTCGGCGGATGTCGCCGCATATG
GAATACCCGAAGGATATGTA

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKPSIVLYKNIPADLRERLEQHFTVHAFDGLTPDNHDELRQALQQAEGIIGSGGKIDEAFLQQTPKLRAASTISVGYDNF
DVDALNAHNVLLMHTPTVLTETVADTIMSLVLATARRVVEVAERVKAGEWQGSIGADWFGVDVHHKTIGILGMGRIGLAL
AQRAHFGFGMPVLYNARRTHEEAEQRFNARRCDLDTLLAESDFICITLPLTDETFHMISRDQLAKMKKSGILINAGRGPV
VDEAALIEALQNGTIHAAGLDVFEKEPLPVSSPLLTLPNVVALPHIGSATHETRYGMAECAVDNLIAALTGTVKENCVNP
QLLKK

Sequences:

>Translated_325_residues
MKPSIVLYKNIPADLRERLEQHFTVHAFDGLTPDNHDELRQALQQAEGIIGSGGKIDEAFLQQTPKLRAASTISVGYDNF
DVDALNAHNVLLMHTPTVLTETVADTIMSLVLATARRVVEVAERVKAGEWQGSIGADWFGVDVHHKTIGILGMGRIGLAL
AQRAHFGFGMPVLYNARRTHEEAEQRFNARRCDLDTLLAESDFICITLPLTDETFHMISRDQLAKMKKSGILINAGRGPV
VDEAALIEALQNGTIHAAGLDVFEKEPLPVSSPLLTLPNVVALPHIGSATHETRYGMAECAVDNLIAALTGTVKENCVNP
QLLKK
>Mature_325_residues
MKPSIVLYKNIPADLRERLEQHFTVHAFDGLTPDNHDELRQALQQAEGIIGSGGKIDEAFLQQTPKLRAASTISVGYDNF
DVDALNAHNVLLMHTPTVLTETVADTIMSLVLATARRVVEVAERVKAGEWQGSIGADWFGVDVHHKTIGILGMGRIGLAL
AQRAHFGFGMPVLYNARRTHEEAEQRFNARRCDLDTLLAESDFICITLPLTDETFHMISRDQLAKMKKSGILINAGRGPV
VDEAALIEALQNGTIHAAGLDVFEKEPLPVSSPLLTLPNVVALPHIGSATHETRYGMAECAVDNLIAALTGTVKENCVNP
QLLKK

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily

Homologues:

Organism=Homo sapiens, GI6912396, Length=321, Percent_Identity=38.6292834890966, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI23308577, Length=290, Percent_Identity=30.3448275862069, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI145580578, Length=276, Percent_Identity=30.0724637681159, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4557499, Length=276, Percent_Identity=30.0724637681159, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI145580575, Length=276, Percent_Identity=30.0724637681159, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI61743967, Length=272, Percent_Identity=30.1470588235294, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4557497, Length=272, Percent_Identity=30.1470588235294, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI87082289, Length=322, Percent_Identity=71.1180124223602, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1787645, Length=194, Percent_Identity=31.9587628865979, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789279, Length=267, Percent_Identity=29.9625468164794, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081824, Length=277, Percent_Identity=28.8808664259928, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17532191, Length=285, Percent_Identity=34.3859649122807, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147481, Length=280, Percent_Identity=28.2142857142857, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=286, Percent_Identity=32.8671328671329, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320925, Length=289, Percent_Identity=30.1038062283737, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=306, Percent_Identity=29.7385620915033, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325144, Length=178, Percent_Identity=34.2696629213483, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321253, Length=278, Percent_Identity=28.0575539568345, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324964, Length=243, Percent_Identity=25.5144032921811, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=162, Percent_Identity=27.1604938271605, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574286, Length=317, Percent_Identity=38.4858044164038, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI28574284, Length=284, Percent_Identity=40.8450704225352, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI45552429, Length=284, Percent_Identity=40.8450704225352, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24585514, Length=284, Percent_Identity=40.8450704225352, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI28574282, Length=284, Percent_Identity=40.8450704225352, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI45551003, Length=284, Percent_Identity=40.8450704225352, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI28571528, Length=259, Percent_Identity=42.0849420849421, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24585516, Length=313, Percent_Identity=32.5878594249201, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI19921140, Length=301, Percent_Identity=30.8970099667774, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24646446, Length=274, Percent_Identity=29.9270072992701, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24646448, Length=274, Percent_Identity=29.9270072992701, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24646452, Length=274, Percent_Identity=29.9270072992701, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24646450, Length=274, Percent_Identity=29.9270072992701, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI62472511, Length=274, Percent_Identity=30.2919708029197, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GHRB_SERP5 (A8G7S7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476295.1
- ProteinModelPortal:   A8G7S7
- SMR:   A8G7S7
- STRING:   A8G7S7
- GeneID:   5604824
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0057
- eggNOG:   COG1052
- HOGENOM:   HBG731446
- OMA:   VLLMHTP
- ProtClustDB:   PRK15409
- BioCyc:   SPRO399741:SPRO_0057-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01667
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.79; =1.1.1.81

Molecular weight: Translated: 35463; Mature: 35463

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 237-237 ACT_SITE 266-266 ACT_SITE 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIVLYKNIPADLRERLEQHFTVHAFDGLTPDNHDELRQALQQAEGIIGSGGKIDEAF
CCCCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
LQQTPKLRAASTISVGYDNFDVDALNAHNVLLMHTPTVLTETVADTIMSLVLATARRVVE
HHCCCCCCCCEEEEECCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWQGSIGADWFGVDVHHKTIGILGMGRIGLALAQRAHFGFGMPVLYNARRTH
HHHHHHCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHHCCCCCHHHCCCHHHH
EEAEQRFNARRCDLDTLLAESDFICITLPLTDETFHMISRDQLAKMKKSGILINAGRGPV
HHHHHHHCCHHCCHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCC
VDEAALIEALQNGTIHAAGLDVFEKEPLPVSSPLLTLPNVVALPHIGSATHETRYGMAEC
CHHHHHHHHHHCCCEEEECCCHHCCCCCCCCCCCHHCCCEEECCCCCCCCHHHHCCHHHH
AVDNLIAALTGTVKENCVNPQLLKK
HHHHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MKPSIVLYKNIPADLRERLEQHFTVHAFDGLTPDNHDELRQALQQAEGIIGSGGKIDEAF
CCCCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHH
LQQTPKLRAASTISVGYDNFDVDALNAHNVLLMHTPTVLTETVADTIMSLVLATARRVVE
HHCCCCCCCCEEEEECCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGEWQGSIGADWFGVDVHHKTIGILGMGRIGLALAQRAHFGFGMPVLYNARRTH
HHHHHHCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHHCCCCCHHHCCCHHHH
EEAEQRFNARRCDLDTLLAESDFICITLPLTDETFHMISRDQLAKMKKSGILINAGRGPV
HHHHHHHCCHHCCHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCC
VDEAALIEALQNGTIHAAGLDVFEKEPLPVSSPLLTLPNVVALPHIGSATHETRYGMAEC
CHHHHHHHHHHCCCEEEECCCHHCCCCCCCCCCCHHCCCEEECCCCCCCCHHHHCCHHHH
AVDNLIAALTGTVKENCVNPQLLKK
HHHHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA