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The map label for this gene is malS [H]

Identifier: 157368303

GI number: 157368303

Start: 53931

End: 55997

Strand: Reverse

Name: malS [H]

Synonym: Spro_0054

Alternate gene names: 157368303

Gene position: 55997-53931 (Counterclockwise)

Preceding gene: 157368306

Following gene: 157368302

Centisome position: 1.03

GC content: 60.14

Gene sequence:

>2067_bases
ATGAAACGCCTGACCCTTCCCCTGTTGATGCTGCTGTCACCGGCGGCAATGGCGAGCTGGACGCTGCAAAGCTTCCCGGC
CTTTGACGAGTCCGCCAGCGGCTTGTTCACCAGCCACGCCACGTTGGCCAAGGGCGAACTGCCGCTGAAGATTTACCAGA
ACCAACAGTGCTGGCAGCCGACCACGGCGGCCAAACTGAATCAAGCCCTGTCACTGCAACCTTGCGGCGATAACCCACCG
GTTAACTGGCGGTTGTTCCGCGCAGGCGAATATCAGGTGCGCATCGACACCCGCAGCGGTACACCAACGTTGCAGCTCAG
CCTGAAAACCGCACCGGAAATCGCCGCCTCTGCGGTAGCGCGCACCTGCCCACGCTGGAACGGCTTGCCGGTCACTCTTG
ACGTGGCGGCAACCTTTGCCGAAGGTGAAACGGTGCGTGATTTCTATTCTGGCCAAACGACCAATGTCAGCCAGGGCAAA
GTCACCCTGCAACCGGCCGCAGGCAGCGGGGGGTTACTGTTGCTGGAGTCGGCAAGCAACGAGAAAGCCGCGCCGTTCAA
CTGGCACAACGCCACGGTCTACTTCGCCCTCACCGATCGCTTTGAAAACGGCAACCCGGCCAACGACCACAGCTACGGCC
GCCGCAGCGACGGCATGCAGGAGATAGGCACCTTCCACGGCGGCGATTTGGCCGGCCTGACGAAAAAGCTCGATTATCTG
CAACAGCTCGGCGTCAATGCGCTGTGGATCAGTTCGCCGCTGGAGCAGATCCACGGCTGGGTTGGTGGTGGCACCAAAGG
CGACTTCCCGCATTACGCCTATCACGGATACTACGCGCTGGACTGGACCCGGCTCGATGCCAACATGGGCAGCGAGCAAG
ATCTGCGCACGCTGGTCGAGCAAGCGCACCAGCGTGGTATCCGCATCCTGTTCGACGTGGTGGTGAATCACGTCGGTTAC
GCCACGCTGGCGGATATGCAGACCTTCCAGTTCGGCTCGCTGTATCTGAAAGGTGCAGAGATCGAAAAAACACTGGGTAA
AAACTGGGGCGACTGGCGGCCGGGCACAGGACAAAACTGGCACAGCTTCAACGATTACATCAACTTCAGCGATAAGGCCG
GCTGGGACGCCTGGTGGGGTAAAAACTGGATCCGCACCGATATAGGCGACTACGATCCACCGGGCTATGACGACCTCACC
ATGTCGCTGGCCTTCCTCCCGGACATCAAAACTGAAGCGTCCGGCGCCAGCGGTCTGCCGCTGTTTTATCGCCATAAACC
CGACACCGCTGCGCACGAAATCCCCGGAGCTACTCCCCGCGACTACCTGACTACCTGGCTCAGCCAATGGGTACGCGATT
ACGGCATTGACGGCTTTCGCGTCGATACCGCCAAGCACGTGGAGAAACCGACGCTGGCGCTGCTGAAACAGCGTGCCACG
GCGGCGTTGGCCGAGTGGAAAGCCGCTCATCCGCAACAGGCACTGGATAACGCCCCATTCTGGATGACCGGTGAAGCCTG
GGGCCATGGCGTGATGAAAAGTGACTATTACCAGAACGGCTTCGACGCGATGATCAATTTTGATTTCCAGGATCAGGCGT
CGCAGGCGCTGAGCTGCTTCTCCAGCATCGACACCACCTATCAACAGATGGCCGACAAGTTGCAGGATTTCAACGTGTTG
AGCTACATCTCTTCGCACGATACCCGGCTGTTCTTTGCCAGTGATGCCAAAGGGTCGCTGGCACTGCAGCAGCGCGCCGC
CGACCTGCTGCTGCTGGCCCCCGGCGCGGTACAAATTTACTACGGTGATGAAAGTGGCCGTCAGCTTGGCCCGAGCGGTT
CAGACCCGTTGCAGGGCACCCGTTCCGATATGAACTGGAGTGAGCTGCAGGGCGACAAAGCCCCACTGCTGGCGCACTGG
CAGCGGCTGGGTCAATTCCGCGCTCGTCACCCGGCAATCGGTGCGGGTAAACAGCAGTCGCAACAAACGGCGCAATACTA
CGCCTTCAGCCGCCGGCTCAGCGACGATAAGGTGATGGTGGTGTGGGTCGGCGATCGGTCACAATAA

Upstream 100 bases:

>100_bases
CCGGAAAATTATCTGTTGGGATGAGGCTATTTTTTTATCGTTGGTCACACTGGGCATTACCCTACGATAAAAACGCTTCC
TGCCACGCGTAAGGAGAGCC

Downstream 100 bases:

>100_bases
TTCAGCGCGATATGCTGGTAAATGGCCATCGGGCGAAGTTTACTCCCGGTGGCTGTTTTATGAACCGCATTGCGTCGGAA
ATTGACAGGCGGTATGGTGG

Product: periplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MKRLTLPLLMLLSPAAMASWTLQSFPAFDESASGLFTSHATLAKGELPLKIYQNQQCWQPTTAAKLNQALSLQPCGDNPP
VNWRLFRAGEYQVRIDTRSGTPTLQLSLKTAPEIAASAVARTCPRWNGLPVTLDVAATFAEGETVRDFYSGQTTNVSQGK
VTLQPAAGSGGLLLLESASNEKAAPFNWHNATVYFALTDRFENGNPANDHSYGRRSDGMQEIGTFHGGDLAGLTKKLDYL
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYALDWTRLDANMGSEQDLRTLVEQAHQRGIRILFDVVVNHVGY
ATLADMQTFQFGSLYLKGAEIEKTLGKNWGDWRPGTGQNWHSFNDYINFSDKAGWDAWWGKNWIRTDIGDYDPPGYDDLT
MSLAFLPDIKTEASGASGLPLFYRHKPDTAAHEIPGATPRDYLTTWLSQWVRDYGIDGFRVDTAKHVEKPTLALLKQRAT
AALAEWKAAHPQQALDNAPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQASQALSCFSSIDTTYQQMADKLQDFNVL
SYISSHDTRLFFASDAKGSLALQQRAADLLLLAPGAVQIYYGDESGRQLGPSGSDPLQGTRSDMNWSELQGDKAPLLAHW
QRLGQFRARHPAIGAGKQQSQQTAQYYAFSRRLSDDKVMVVWVGDRSQ

Sequences:

>Translated_688_residues
MKRLTLPLLMLLSPAAMASWTLQSFPAFDESASGLFTSHATLAKGELPLKIYQNQQCWQPTTAAKLNQALSLQPCGDNPP
VNWRLFRAGEYQVRIDTRSGTPTLQLSLKTAPEIAASAVARTCPRWNGLPVTLDVAATFAEGETVRDFYSGQTTNVSQGK
VTLQPAAGSGGLLLLESASNEKAAPFNWHNATVYFALTDRFENGNPANDHSYGRRSDGMQEIGTFHGGDLAGLTKKLDYL
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYALDWTRLDANMGSEQDLRTLVEQAHQRGIRILFDVVVNHVGY
ATLADMQTFQFGSLYLKGAEIEKTLGKNWGDWRPGTGQNWHSFNDYINFSDKAGWDAWWGKNWIRTDIGDYDPPGYDDLT
MSLAFLPDIKTEASGASGLPLFYRHKPDTAAHEIPGATPRDYLTTWLSQWVRDYGIDGFRVDTAKHVEKPTLALLKQRAT
AALAEWKAAHPQQALDNAPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQASQALSCFSSIDTTYQQMADKLQDFNVL
SYISSHDTRLFFASDAKGSLALQQRAADLLLLAPGAVQIYYGDESGRQLGPSGSDPLQGTRSDMNWSELQGDKAPLLAHW
QRLGQFRARHPAIGAGKQQSQQTAQYYAFSRRLSDDKVMVVWVGDRSQ
>Mature_688_residues
MKRLTLPLLMLLSPAAMASWTLQSFPAFDESASGLFTSHATLAKGELPLKIYQNQQCWQPTTAAKLNQALSLQPCGDNPP
VNWRLFRAGEYQVRIDTRSGTPTLQLSLKTAPEIAASAVARTCPRWNGLPVTLDVAATFAEGETVRDFYSGQTTNVSQGK
VTLQPAAGSGGLLLLESASNEKAAPFNWHNATVYFALTDRFENGNPANDHSYGRRSDGMQEIGTFHGGDLAGLTKKLDYL
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYALDWTRLDANMGSEQDLRTLVEQAHQRGIRILFDVVVNHVGY
ATLADMQTFQFGSLYLKGAEIEKTLGKNWGDWRPGTGQNWHSFNDYINFSDKAGWDAWWGKNWIRTDIGDYDPPGYDDLT
MSLAFLPDIKTEASGASGLPLFYRHKPDTAAHEIPGATPRDYLTTWLSQWVRDYGIDGFRVDTAKHVEKPTLALLKQRAT
AALAEWKAAHPQQALDNAPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQASQALSCFSSIDTTYQQMADKLQDFNVL
SYISSHDTRLFFASDAKGSLALQQRAADLLLLAPGAVQIYYGDESGRQLGPSGSDPLQGTRSDMNWSELQGDKAPLLAHW
QRLGQFRARHPAIGAGKQQSQQTAQYYAFSRRLSDDKVMVVWVGDRSQ

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=684, Percent_Identity=69.2982456140351, Blast_Score=987, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322245, Length=190, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 76358; Mature: 76358

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLTLPLLMLLSPAAMASWTLQSFPAFDESASGLFTSHATLAKGELPLKIYQNQQCWQP
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEECCCEEECCCCCEEEECCCCCCCC
TTAAKLNQALSLQPCGDNPPVNWRLFRAGEYQVRIDTRSGTPTLQLSLKTAPEIAASAVA
HHHHHHHHCEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEECCCHHHHHHHHH
RTCPRWNGLPVTLDVAATFAEGETVRDFYSGQTTNVSQGKVTLQPAAGSGGLLLLESASN
HHCCCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEECCCC
EKAAPFNWHNATVYFALTDRFENGNPANDHSYGRRSDGMQEIGTFHGGDLAGLTKKLDYL
CCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHH
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYALDWTRLDANMGSEQDLRTLVE
HHCCCCEEEECCHHHHHCCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCHHHHHHHHH
QAHQRGIRILFDVVVNHVGYATLADMQTFQFGSLYLKGAEIEKTLGKNWGDWRPGTGQNW
HHHHCCHHHHHHHHHHHCCCHHHHHHHHEEECCEEEECCHHHHHHCCCCCCCCCCCCCCC
HSFNDYINFSDKAGWDAWWGKNWIRTDIGDYDPPGYDDLTMSLAFLPDIKTEASGASGLP
CCHHCCCCCCCCCCCCHHHCCCCEECCCCCCCCCCCCHHEEEEEECCCCCCCCCCCCCCC
LFYRHKPDTAAHEIPGATPRDYLTTWLSQWVRDYGIDGFRVDTAKHVEKPTLALLKQRAT
EEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCHHHHHHHHHHH
AALAEWKAAHPQQALDNAPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQASQALSCF
HHHHHHHCCCCHHHHCCCCEEEECHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
SSIDTTYQQMADKLQDFNVLSYISSHDTRLFFASDAKGSLALQQRAADLLLLAPGAVQIY
HHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCEEEECCCEEEEE
YGDESGRQLGPSGSDPLQGTRSDMNWSELQGDKAPLLAHWQRLGQFRARHPAIGAGKQQS
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
QQTAQYYAFSRRLSDDKVMVVWVGDRSQ
HHHHHHHHHHHCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MKRLTLPLLMLLSPAAMASWTLQSFPAFDESASGLFTSHATLAKGELPLKIYQNQQCWQP
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEECCCEEECCCCCEEEECCCCCCCC
TTAAKLNQALSLQPCGDNPPVNWRLFRAGEYQVRIDTRSGTPTLQLSLKTAPEIAASAVA
HHHHHHHHCEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEECCCHHHHHHHHH
RTCPRWNGLPVTLDVAATFAEGETVRDFYSGQTTNVSQGKVTLQPAAGSGGLLLLESASN
HHCCCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEECCCC
EKAAPFNWHNATVYFALTDRFENGNPANDHSYGRRSDGMQEIGTFHGGDLAGLTKKLDYL
CCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHH
QQLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYALDWTRLDANMGSEQDLRTLVE
HHCCCCEEEECCHHHHHCCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCHHHHHHHHH
QAHQRGIRILFDVVVNHVGYATLADMQTFQFGSLYLKGAEIEKTLGKNWGDWRPGTGQNW
HHHHCCHHHHHHHHHHHCCCHHHHHHHHEEECCEEEECCHHHHHHCCCCCCCCCCCCCCC
HSFNDYINFSDKAGWDAWWGKNWIRTDIGDYDPPGYDDLTMSLAFLPDIKTEASGASGLP
CCHHCCCCCCCCCCCCHHHCCCCEECCCCCCCCCCCCHHEEEEEECCCCCCCCCCCCCCC
LFYRHKPDTAAHEIPGATPRDYLTTWLSQWVRDYGIDGFRVDTAKHVEKPTLALLKQRAT
EEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCHHHHHHHHHHH
AALAEWKAAHPQQALDNAPFWMTGEAWGHGVMKSDYYQNGFDAMINFDFQDQASQALSCF
HHHHHHHCCCCHHHHCCCCEEEECHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
SSIDTTYQQMADKLQDFNVLSYISSHDTRLFFASDAKGSLALQQRAADLLLLAPGAVQIY
HHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCEEEECCCEEEEE
YGDESGRQLGPSGSDPLQGTRSDMNWSELQGDKAPLLAHWQRLGQFRARHPAIGAGKQQS
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
QQTAQYYAFSRRLSDDKVMVVWVGDRSQ
HHHHHHHHHHHCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]