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The map label for this gene is trmE [H]

Identifier: 157368276

GI number: 157368276

Start: 26077

End: 27441

Strand: Reverse

Name: trmE [H]

Synonym: Spro_0027

Alternate gene names: 157368276

Gene position: 27441-26077 (Counterclockwise)

Preceding gene: 157368277

Following gene: 157368275

Centisome position: 0.5

GC content: 61.03

Gene sequence:

>1365_bases
ATGAGCACCACCGATACCATCGTAGCCCAAGCCACCCCGCCGGGACGCGGCGGCGTCGGCATTCTGCGTATTTCCGGCCG
TGGCGCCAAAGATGTCGCCCAGGCCCTGCTCGGCAAGCTACCCAAGCCGCGTTACGCCGATTACCTGCCATTCCGGGATG
CCGCAGGTGCCACCCTGGATCAGGGCATCGCACTGTGGTTCCCAGGCCCTAATTCGTTTACCGGCGAAGACGTGCTGGAG
CTGCAAGGCCACGGCGGGCCGGTGATCCTCGATCTGCTGCTCAAACGCGTGTTGGCGCTGCCAGGCATGCGTATCGCGCG
GCCCGGCGAGTTTTCCGAACGCGCATTCCTCAACGACAAGCTGGATCTGGCGCAGGCCGAGGCGATTGCCGACCTGATCG
ATGCCAGTTCCGAGCAGGCGGCACGTTCAGCCATGAACTCGCTGCAGGGGGCTTTTTCCACCCGCATCCATCAGCTTGTG
GAAGCACTTACTCACCTGCGGATCTATGTGGAAGCGGCCATCGACTTCCCCGATGAGGAAATCGACTTCCTGTCTGACGG
TAAAATCGAAGCGCAGCTCAACGGCGTTATGGCCGATCTGGACAGCGTACGCGCCGAAGCGCGCCAGGGCAGCCTGCTGC
GTGAAGGGATGAAGGTGGTGATCGCCGGGCGGCCAAACGCCGGTAAATCCAGCCTGCTGAACGCATTGGCCGGCCGCGAG
GCGGCGATTGTTACCGATATTGCCGGCACCACCCGCGACGTTTTGCGCGAACATATTCACATCGACGGCATGCCATTGCA
CATCATCGACACCGCCGGCCTGCGTGAAGCCAGCGACGAAGTTGAGCGCATCGGTATCGAACGTGCATGGAACGAGATCG
AACAGGCGGATCGCGTGCTGTTTATGGTCGATGGCACCACCACCGCGGCCACCGAACCGGCTGAGATTTGGCCAGAGTTT
ATGGCACGTCTGCCGCACCGCTTGCCAATCACCGTGGTGCGTAATAAAGCCGACATCACCGGTGAAACGCTGGGTATGAC
CGAAGTAAATGGCCACTCACTTATTCGCCTGTCGGCGCGTACCGGTGAAGGCATCGATCTGCTGCGCGACCACCTGAAGC
AGAGCATGGGCTTCACCAGCAATATGGAAGGTGGCTTCCTGGCGCGCCGTCGCCATCTGCAGGCGCTTGAGCAAGCGGCC
CAGCATCTGGTCGAGGGCAAAGAACAGTTAGTAAGCGCTTACGCGGGGGAGCTGCTGGCCGAGGAATTGCGCCTGGCGCA
GCAAGCACTGAGTGAAATCACCGGCGAATTTACCTCCGATGATCTGCTGGGGCGCATCTTCTCCAGCTTCTGTATCGGCA
AGTAA

Upstream 100 bases:

>100_bases
GTATTGTAGGGGTTCGGCGCGCTGAACCCTTGCAGTAACACCAAGAGGCGGTCATTAGACCGCCTTTTTGCATATTGAAT
TGTCAGAGAAGAGAACCACT

Downstream 100 bases:

>100_bases
ACCCTCATCCTTCTAAGGAAAGAACAGGCATGGCATTAACGCTCAGGGTGTTACTGGAAAACCGCCGCGCCACCGGCGTG
GACAAATCGCTGCGGGTGAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSTTDTIVAQATPPGRGGVGILRISGRGAKDVAQALLGKLPKPRYADYLPFRDAAGATLDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRVLALPGMRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSAMNSLQGAFSTRIHQLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNGVMADLDSVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWNEIEQADRVLFMVDGTTTAATEPAEIWPEF
MARLPHRLPITVVRNKADITGETLGMTEVNGHSLIRLSARTGEGIDLLRDHLKQSMGFTSNMEGGFLARRRHLQALEQAA
QHLVEGKEQLVSAYAGELLAEELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK

Sequences:

>Translated_454_residues
MSTTDTIVAQATPPGRGGVGILRISGRGAKDVAQALLGKLPKPRYADYLPFRDAAGATLDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRVLALPGMRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSAMNSLQGAFSTRIHQLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNGVMADLDSVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWNEIEQADRVLFMVDGTTTAATEPAEIWPEF
MARLPHRLPITVVRNKADITGETLGMTEVNGHSLIRLSARTGEGIDLLRDHLKQSMGFTSNMEGGFLARRRHLQALEQAA
QHLVEGKEQLVSAYAGELLAEELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK
>Mature_453_residues
STTDTIVAQATPPGRGGVGILRISGRGAKDVAQALLGKLPKPRYADYLPFRDAAGATLDQGIALWFPGPNSFTGEDVLEL
QGHGGPVILDLLLKRVLALPGMRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSAMNSLQGAFSTRIHQLVE
ALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNGVMADLDSVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREA
AIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWNEIEQADRVLFMVDGTTTAATEPAEIWPEFM
ARLPHRLPITVVRNKADITGETLGMTEVNGHSLIRLSARTGEGIDLLRDHLKQSMGFTSNMEGGFLARRRHLQALEQAAQ
HLVEGKEQLVSAYAGELLAEELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=467, Percent_Identity=35.3319057815846, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=34.2, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=34.4902386117137, Blast_Score=225, Evalue=6e-59,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=89.647577092511, Blast_Score=828, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=39.5604395604396, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=27.9220779220779, Blast_Score=164, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=31.6326530612245, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI45550813, Length=482, Percent_Identity=31.9502074688797, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49160; Mature: 49028

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTDTIVAQATPPGRGGVGILRISGRGAKDVAQALLGKLPKPRYADYLPFRDAAGATLD
CCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRVLALPGMRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHHCCCH
LDLAQAEAIADLIDASSEQAARSAMNSLQGAFSTRIHQLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
IDFLSDGKIEAQLNGVMADLDSVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
HCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWNEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTAATEPAEIWPEFMARLPHRLPITVVRNKADITGETLGMTEVNGHSLIRLSAR
EEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECCCEEEEEECC
TGEGIDLLRDHLKQSMGFTSNMEGGFLARRRHLQALEQAAQHLVEGKEQLVSAYAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STTDTIVAQATPPGRGGVGILRISGRGAKDVAQALLGKLPKPRYADYLPFRDAAGATLD
CCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRVLALPGMRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHHCCCH
LDLAQAEAIADLIDASSEQAARSAMNSLQGAFSTRIHQLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
IDFLSDGKIEAQLNGVMADLDSVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
HCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWNEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTAATEPAEIWPEFMARLPHRLPITVVRNKADITGETLGMTEVNGHSLIRLSAR
EEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECCCEEEEEECC
TGEGIDLLRDHLKQSMGFTSNMEGGFLARRRHLQALEQAAQHLVEGKEQLVSAYAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQALSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA