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The map label for this gene is gltJ [H]

Identifier: 157368267

GI number: 157368267

Start: 17162

End: 17875

Strand: Direct

Name: gltJ [H]

Synonym: Spro_0018

Alternate gene names: 157368267

Gene position: 17162-17875 (Clockwise)

Preceding gene: 157368266

Following gene: 157368268

Centisome position: 0.31

GC content: 59.94

Gene sequence:

>714_bases
ATGAATCTGGACTGGCTGCTGGCGCCGGAGTATCTGAGCTGGCTGTGGCGGGGTTTCCTGCTGACGCTGTGGCTTTCTGC
CTGCGCGAGCCTGGCCGCCACGCTGTTGGGGTTTTTACTGACCGCGATGCGTGACAGCCGCCTGCGGGCACTGCGTTGGC
TGGCGGTGGGTTACAGTTCGTTGTTTCGCAATACGCCGTTGCTGGTACAGCTGTTCTTCTGGTATTTCGCCGCCGGGCAG
ATCCTGCCGTCCGCCGCCATGCAGTGGCTGAATACTTCACATCAAATCGGCCCGCTTGAGTGGCCGTCGTTTGAATTTCT
CGCCGGTTTTTTCGGCCTGACGCTGTACTCCAGCGCTTTTATCGCCGAAGAGATCCGTTCCGGCATCCGTGGTGTCGCCG
GTGGGCAAAAATATGCGGCCAACGCGCTGGGATTAACCGGCTGGCAGGCGATGCGCTATGTGGTGCTGCCACAGGCGCTG
AAAATCGCCATGCCGCCGCTGCTGGGCCAGTATATGAACGTGATAAAAAACTCCTCGCTGACCATGGCGATCGGCGTTGC
CGAACTGTCCTATGCTTCGCGTCAGGTGGAAACCGAGACGCTGCGCACCTTCCAGGCGTTTGGCGTGGCGACGGTGCTGT
ACATCGCCATCATTGCGCTGTTGGAAGGCTGGGGTATGTGGCGACAGCAGCGTAAACCGCTGAGAGGGCACTAA

Upstream 100 bases:

>100_bases
TGGATCAACAGCCGAAGGCCTGATTTTCACGGGCTGAAATCTGTTTGGAGGGGCGCAGCTTGCTGCGCCCCTGACGCATT
TTGCAAGACAAGGATTGACC

Downstream 100 bases:

>100_bases
GATGGATTTCAGCGTCATTTACGACAATCTGGGTTATCTGCTGTGGGGCACCTGGCCGGACGGCCCGCTGGGCGGCGCAG
CGCTGACGTTGGCGATCAGC

Product: polar amino acid ABC transporter inner membrane subunit

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MNLDWLLAPEYLSWLWRGFLLTLWLSACASLAATLLGFLLTAMRDSRLRALRWLAVGYSSLFRNTPLLVQLFFWYFAAGQ
ILPSAAMQWLNTSHQIGPLEWPSFEFLAGFFGLTLYSSAFIAEEIRSGIRGVAGGQKYAANALGLTGWQAMRYVVLPQAL
KIAMPPLLGQYMNVIKNSSLTMAIGVAELSYASRQVETETLRTFQAFGVATVLYIAIIALLEGWGMWRQQRKPLRGH

Sequences:

>Translated_237_residues
MNLDWLLAPEYLSWLWRGFLLTLWLSACASLAATLLGFLLTAMRDSRLRALRWLAVGYSSLFRNTPLLVQLFFWYFAAGQ
ILPSAAMQWLNTSHQIGPLEWPSFEFLAGFFGLTLYSSAFIAEEIRSGIRGVAGGQKYAANALGLTGWQAMRYVVLPQAL
KIAMPPLLGQYMNVIKNSSLTMAIGVAELSYASRQVETETLRTFQAFGVATVLYIAIIALLEGWGMWRQQRKPLRGH
>Mature_237_residues
MNLDWLLAPEYLSWLWRGFLLTLWLSACASLAATLLGFLLTAMRDSRLRALRWLAVGYSSLFRNTPLLVQLFFWYFAAGQ
ILPSAAMQWLNTSHQIGPLEWPSFEFLAGFFGLTLYSSAFIAEEIRSGIRGVAGGQKYAANALGLTGWQAMRYVVLPQAL
KIAMPPLLGQYMNVIKNSSLTMAIGVAELSYASRQVETETLRTFQAFGVATVLYIAIIALLEGWGMWRQQRKPLRGH

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=212, Percent_Identity=34.4339622641509, Blast_Score=130, Evalue=5e-32,
Organism=Escherichia coli, GI1788226, Length=230, Percent_Identity=33.4782608695652, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787030, Length=232, Percent_Identity=30.6034482758621, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1786873, Length=187, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI48994930, Length=114, Percent_Identity=41.2280701754386, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788645, Length=227, Percent_Identity=27.3127753303965, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082239, Length=184, Percent_Identity=29.3478260869565, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787086, Length=214, Percent_Identity=28.5046728971963, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787087, Length=221, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 26578; Mature: 26578

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDWLLAPEYLSWLWRGFLLTLWLSACASLAATLLGFLLTAMRDSRLRALRWLAVGYSS
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRNTPLLVQLFFWYFAAGQILPSAAMQWLNTSHQIGPLEWPSFEFLAGFFGLTLYSSAF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IAEEIRSGIRGVAGGQKYAANALGLTGWQAMRYVVLPQALKIAMPPLLGQYMNVIKNSSL
HHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCH
TMAIGVAELSYASRQVETETLRTFQAFGVATVLYIAIIALLEGWGMWRQQRKPLRGH
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNLDWLLAPEYLSWLWRGFLLTLWLSACASLAATLLGFLLTAMRDSRLRALRWLAVGYSS
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRNTPLLVQLFFWYFAAGQILPSAAMQWLNTSHQIGPLEWPSFEFLAGFFGLTLYSSAF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IAEEIRSGIRGVAGGQKYAANALGLTGWQAMRYVVLPQALKIAMPPLLGQYMNVIKNSSL
HHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCH
TMAIGVAELSYASRQVETETLRTFQAFGVATVLYIAIIALLEGWGMWRQQRKPLRGH
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]