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The map label for this gene is phnD

Identifier: 157159457

GI number: 157159457

Start: 4655233

End: 4656249

Strand: Reverse

Name: phnD

Synonym: EcE24377A_4658

Alternate gene names: 157159457

Gene position: 4656249-4655233 (Counterclockwise)

Preceding gene: 157158129

Following gene: 157157797

Centisome position: 93.51

GC content: 54.67

Gene sequence:

>1017_bases
ATGAACGCTAAGATAATTGCCTCGCTGGCCTTCACCAGCATGTTCAGCCTCAGCACCCTGTTAAGCCCGGCACACGCCGA
AGAGCAGGAAAAGGCGCTGAATTTCGGCATTATTTCAACGGAATCACAGCAAAACCTGAAACCGCAATGGACGCCATTCT
TACAGGATATGGAGAAGAAGCTGGGCGTGAAGGTGAACGCCTTCTTTGCCCCAGACTACGCAGGCATTATCCAGGGAATG
CGCTTCAATAAAGTGGATATCGCCTGGTACGGCAACCTGTCGGCAATGGAAGCGGTGGATCGCGCCAACGGCCAGGTCTT
CGCCCAGACGGTCGCGGCGGATGGATCGCCAGGTTACTGGAGCGTGTTGATCGTCAACAAAGACAGCCCGATCAACAACC
TGAACGATCTGCTGGCGAAGCGGAAAGATCTCACCTTCGGCAATGGCGATCCTAACTCCACCTCTGGCTTCCTCGTCCCC
GGCTACTACGTCTTCGCCAAAAACAATATCTCCGCCAGCGACTTCAAGCGCACCGTCAACGCCGGGCATGAAACCAACGC
GCTGGCCGTCGCCAACAAGCAGGTGGATGTTGCCACTAACAACACCGAAAACCTCGACAAGCTGAAAACCTCCGCGCCGG
AGAAGTTGAAAGAACTGAAAGTGATCTGGAAGTCGCCGCTGATCCCAGGCGATCCGATCGTCTGGCGTAAAAACCTTTCC
GAAACCACCAAAGACAAGATCTACGACTTCTTTATGAACTACGGAAAAACGCCGGAAGAAAAAGCGGTGCTGGAACGCCT
GGGCTGGGCGCCGTTCCGCGCTTCCAGCGACCTGCAACTGGTGCCGATTCGCCAGCTCGCACTGTTTAAAGAGATGCAGG
GCGTAAAAAGCAATAAAGGACTGAATGAGCAGGACAAGCTGGCGAAAACCACCGAGATTCAGGCGCAGCTGGATGACCTG
GACCGCCTGAACAACGCGTTAAGCGCGATGAGTTCGGTGAGTAAAGCGGTGCAGTAA

Upstream 100 bases:

>100_bases
ACAGTTTGATAACGAACGTTTTGACCATCTCTACCGCAGCATTAATCGCATCGAAGAGAACGCGAAAGCTGCCTGACATC
CCCATCATTGAGGAAAACGA

Downstream 100 bases:

>100_bases
TGTGCTCGATCGGTCCCCTCTCCCTTGAGGGAGAGGGTTAGGGTGAGGGGGAATGCGCACGCTGATGCCCTCACCCCGGC
CCTCTCCCACAGGGAGAGGG

Product: phosphonate ABC transporter, periplasmic phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQNLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGM
RFNKVDIAWYGNLSAMEAVDRANGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKELKVIWKSPLIPGDPIVWRKNLS
ETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASSDLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDL
DRLNNALSAMSSVSKAVQ

Sequences:

>Translated_338_residues
MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQNLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGM
RFNKVDIAWYGNLSAMEAVDRANGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKELKVIWKSPLIPGDPIVWRKNLS
ETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASSDLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDL
DRLNNALSAMSSVSKAVQ
>Mature_338_residues
MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQNLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGM
RFNKVDIAWYGNLSAMEAVDRANGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKELKVIWKSPLIPGDPIVWRKNLS
ETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASSDLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDL
DRLNNALSAMSSVSKAVQ

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=338, Percent_Identity=100, Blast_Score=689, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHND_ECOLI (P16682)

Other databases:

- EMBL:   J05260
- EMBL:   D90227
- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S56333
- RefSeq:   AP_004606.1
- RefSeq:   NP_418529.1
- ProteinModelPortal:   P16682
- SMR:   P16682
- DIP:   DIP-10483N
- STRING:   P16682
- EnsemblBacteria:   EBESCT00000003589
- EnsemblBacteria:   EBESCT00000015123
- GeneID:   948624
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW4066
- KEGG:   eco:b4105
- EchoBASE:   EB0708
- EcoGene:   EG10714
- eggNOG:   COG3221
- GeneTree:   EBGT00050000011377
- HOGENOM:   HBG373233
- OMA:   QLLPIRQ
- ProtClustDB:   CLSK867942
- BioCyc:   EcoCyc:PHND-MONOMER
- Genevestigator:   P16682
- InterPro:   IPR017797
- InterPro:   IPR005770
- TIGRFAMs:   TIGR01098
- TIGRFAMs:   TIGR03431

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37371; Mature: 37371

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQNLKPQWTPFLQDMEKK
CCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHH
LGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRANGQVFAQTVAADGSPGYW
HCCEEEEEECCCHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCEE
SVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVPGYYVFAKNNISASDFKRTVN
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHC
AGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKELKVIWKSPLIPGDPIVWRKNLS
CCCCCCEEEEECCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
ETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASSDLQLVPIRQLALFKEMQGVKSNKG
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCC
LNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVSKAVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQNLKPQWTPFLQDMEKK
CCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHH
LGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRANGQVFAQTVAADGSPGYW
HCCEEEEEECCCHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCEE
SVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVPGYYVFAKNNISASDFKRTVN
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHC
AGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKELKVIWKSPLIPGDPIVWRKNLS
CCCCCCEEEEECCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
ETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASSDLQLVPIRQLALFKEMQGVKSNKG
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCC
LNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVSKAVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503