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The map label for this gene is phnE

Identifier: 157157797

GI number: 157157797

Start: 4654329

End: 4655108

Strand: Reverse

Name: phnE

Synonym: EcE24377A_4657

Alternate gene names: 157157797

Gene position: 4655108-4654329 (Counterclockwise)

Preceding gene: 157159457

Following gene: 157156155

Centisome position: 93.48

GC content: 61.54

Gene sequence:

>780_bases
ATGCAAACCATCACCATCGCCCCACCCAAGCGTAGCTGGTTCTCGCTTCTGAGCTGGGCCGTTGTTCTCGCCGTGCTGGT
CGTCTCGTGGCAGGGCGCGGAAATGGCCCCGCTTACGCTGATTAAAGACGGCGGCAACATGGCAACCTTCGCTGCCGACT
TCTTCCCGCCCGATTTCAGCCAGTGGCAGGATTACCTCACCGAAATGGCCGTCACGCTGCAAATCGCCGTCTGGGGCACC
GCGCTGGCAGTGGTTCTCTCCATCCCCTTCGGCCTGATGAGCGCCGAAAACCTGGTGCCGTGGTGGGTTTACCAGCCCGT
TCGCCGCCTGATGGACGCCTGCCGCGCCATTAACGAAATGGTCTTCGCCATGCTGTTCGTGGTCGCCGTCGGTCTCGGAC
CGTTCGCTGGCGTGCTGGCGCTATTTATCCACACCACCGGCGTGCTCTCCAAGCTGCTTTCCGAGGCGGTGGAAGCGATT
GAACCCGGCCCGGTGGAAGGCATTCGCGCCACCGGTGCCAACAAGCTCGAAGAGATCCTCTACGGCGTGCTGCCACAGGT
GATGCCGCTGCTGATCTCCTACTCCCTCTATCGCTTCGAATCCAACGTCCGCTCGGCGACCGTCGTCGGCATGGTCGGCG
CAGGCGGGATCGGCGTCACCCTGTGGGAAGCGATTCGCGGTTTCCAGTTCCAGCAAACCTGCGCCCTGATGGTGCTGATT
ATCGTCACGGTCAGCCTGCTGGATTTCCTCTCTCAACGGTTGCGTAAGCACTTTATCTGA

Upstream 100 bases:

>100_bases
TTGAGGGAGAGGGTTAGGGTGAGGGGGAATGCGCACGCTGATGCCCTCACCCCGGCCCTCTCCCACAGGGAGAGGGAGAA
AACATTAAACGGAGCCAAAC

Downstream 100 bases:

>100_bases
TAAGCGAGGCATTGATATCTATGCACTTGTCTACACATCCGACCAGCTATCCAACACGCTATCAGGAGATAGCCGCAAAA
CTTGAGCAGGAGCTTCGTCA

Product: phosphonate ABC transporter, permease protein

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGT
ALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
IVTVSLLDFLSQRLRKHFI

Sequences:

>Translated_259_residues
MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGT
ALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
IVTVSLLDFLSQRLRKHFI
>Mature_259_residues
MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGT
ALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
IVTVSLLDFLSQRLRKHFI

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHNE_ECOLI (P16683)

Other databases:

- EMBL:   J05260
- EMBL:   D90227
- EMBL:   D90227
- EMBL:   U14003
- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F35718
- ProteinModelPortal:   P16683
- EnsemblBacteria:   EBESCT00000018404
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- EchoBASE:   EB1260
- EcoGene:   EG11283
- GeneTree:   EBGT00050000011537
- HOGENOM:   HBG642273
- OMA:   WALVWIN
- BioCyc:   EcoCyc:G7822-MONOMER
- BioCyc:   EcoCyc:PHNE-MONOMER
- Genevestigator:   P16683
- InterPro:   IPR000515
- InterPro:   IPR005769
- TIGRFAMs:   TIGR01097

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 28384; Mature: 28384

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x221e0b78)-; HASH(0x229e9818)-; HASH(0x224d7b18)-; HASH(0x1f409ad4)-; HASH(0x226ca3c4)-; HASH(0x227ff918)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDFS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEHHHHCCCCCHH
QWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEIL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHH
YGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHH
IVTVSLLDFLSQRLRKHFI
HHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDFS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEHHHHCCCCCHH
QWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEIL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHH
YGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHH
IVTVSLLDFLSQRLRKHFI
HHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503