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The map label for this gene is pdxB

Identifier: 157159447

GI number: 157159447

Start: 2607157

End: 2608293

Strand: Reverse

Name: pdxB

Synonym: EcE24377A_2614

Alternate gene names: 157159447

Gene position: 2608293-2607157 (Counterclockwise)

Preceding gene: 157157524

Following gene: 157154856

Centisome position: 52.38

GC content: 52.07

Gene sequence:

>1137_bases
GTGAAAATCCTTGTTGATGAAAATATGCCTTATGCCCGCGAATTATTTAGTCGCCTGGGGGAAGTGAAAGCAGTTCCTGG
ACGCCCGATTCCGGTTGTACAACTGGATGATGCAGATGCGCTGATGGTGCGTTCGGTGACGAAAGTGAATGAATCTTTGC
TGGCAGGAAAACCCATTAAATTTGTTGGCACTGCCACTGCGGGGACAGACCATGTCGATGAAGTATGGTTAAAGCAGGCG
GGAATTGGTTTTTCCGCGGCACCTGGCTGTAATGCGATTGCGGTGGTGGAATATGTTTTCTCCTCCCTGCTGATGCTTGC
CGAACGCGATGGATTTTCACTGCACGAGCGTACCGTGGGGATCGTGGGCGTTGGTAACGTTGGTCGGCGTTTACAGGCAC
GACTGGAAGTGTTAGGGATTAAAACCTTACTTTGCGATCCGCCTCGCGCTGACCGTGGGGATGAGGGTGATTTCCGCTCG
CTGGATGAATTAGTTCAGCATGCGGATATTCTGACTTTCCATACGCCACTCTTCAAAGACGGGCCGTACAAAACGCTGCA
TCTGGCGGATGAAAAACTGATCCGTAGCCTGAAGCCGGGAGCGATTCTGATTAACGCCTGCCGTGGCGCAGTCGTCGATA
ATACTGCGTTGCTGACCTGCCTGAATGAAGGCCAGAAGTTAAGCGTAGTGCTGGATGTCTGGGAAGGCGAACCGGAACTC
AACGTCGAGCTGCTGAAAAAAGTGGATATCGGCACGCCGCATATCGCAGGTTATACCCTGGAAGGTAAAGCACGCGGTAC
TACGCAAGTGTTTGAAGCCTATAGCAAGTTTATTGGGCATGAACAGCACGTTGCGCTGGATACATTACTGCCCGCGCCAG
AGTTTGGTCGCATTACGCTGCATGGCCCGCTCGATCAACCGACGCTGAAAAGGCTGGTGCATTTGGTGTATGATGTGCGC
CGCGATGACGCACCGCTGCGTAAAGTCGCCGGGATACCGGGTGAGTTCGATAAACTGCGCAAAAACTATCTTGAGCGCCG
TGAATGGTCATCTCTGTATGTAATTTGTGATGACGCCAGTGCGGCATCATTGCTGTGTAAACTGGGTTTTAACGCCGTTC
ATCATCCGGCACGTTAA

Upstream 100 bases:

>100_bases
TCACTTATCCTTAAATCATAAATCTTCGTAAGTATGCCTGACGGCGAGATTATGGCACACTTGCCCGGTTAACTCTCGTT
CTCAAACAGGTAACACAAAC

Downstream 100 bases:

>100_bases
TCTCTTCTTCATGCTCTCTGCTGTAACATGGGCAGGGAGCTTTGCTATTTCTGGAGTAAACCACCATGTCTGAAGGCTGG
AACATTGCCGTCCTGGGCGC

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKILVDENMPYARELFSRLGEVKAVPGRPIPVVQLDDADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEVWLKQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEVLGIKTLLCDPPRADRGDEGDFRS
LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVR
RDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR

Sequences:

>Translated_378_residues
MKILVDENMPYARELFSRLGEVKAVPGRPIPVVQLDDADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEVWLKQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEVLGIKTLLCDPPRADRGDEGDFRS
LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVR
RDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR
>Mature_378_residues
MKILVDENMPYARELFSRLGEVKAVPGRPIPVVQLDDADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEVWLKQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEVLGIKTLLCDPPRADRGDEGDFRS
LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVR
RDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=257, Percent_Identity=29.9610894941634, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI61743967, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4557497, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI145580578, Length=276, Percent_Identity=25.7246376811594, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4557499, Length=276, Percent_Identity=25.7246376811594, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI145580575, Length=276, Percent_Identity=25.7246376811594, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI6912396, Length=316, Percent_Identity=25.9493670886076, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1788660, Length=378, Percent_Identity=97.3544973544974, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI1787645, Length=205, Percent_Identity=25.8536585365854, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1789279, Length=305, Percent_Identity=28.1967213114754, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082289, Length=159, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=270, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=323, Percent_Identity=27.8637770897833, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322116, Length=323, Percent_Identity=27.5541795665635, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324055, Length=300, Percent_Identity=27.3333333333333, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324964, Length=264, Percent_Identity=25.7575757575758, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921140, Length=273, Percent_Identity=26.007326007326, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24646446, Length=253, Percent_Identity=26.4822134387352, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24646448, Length=253, Percent_Identity=26.4822134387352, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24646452, Length=253, Percent_Identity=26.4822134387352, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24646450, Length=253, Percent_Identity=26.4822134387352, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI62472511, Length=253, Percent_Identity=26.8774703557312, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_ECO24 (A7ZPD6)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463665.1
- ProteinModelPortal:   A7ZPD6
- SMR:   A7ZPD6
- STRING:   A7ZPD6
- EnsemblBacteria:   EBESCT00000019045
- GeneID:   5590697
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2614
- NMPDR:   fig|331111.3.peg.351
- eggNOG:   COG0111
- GeneTree:   EBGT00050000009079
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- ProtClustDB:   PRK15438
- BioCyc:   ECOL331111:ECE24377A_2614-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 41516; Mature: 41516

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 208-208 ACT_SITE 237-237 ACT_SITE 254-254 BINDING 45-45 BINDING 66-66 BINDING 146-146 BINDING 175-175 BINDING 232-232 BINDING 257-257 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVDENMPYARELFSRLGEVKAVPGRPIPVVQLDDADALMVRSVTKVNESLLAGKPIK
CEEEECCCCCHHHHHHHHHCCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEE
FVGTATAGTDHVDEVWLKQAGIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVG
EEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCEEE
IVGVGNVGRRLQARLEVLGIKTLLCDPPRADRGDEGDFRSLDELVQHADILTFHTPLFKD
EEECCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
GPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
CCCCEEEHHHHHHHHHCCCCEEEEECCCCCEECCCEEEEEECCCCEEEEEEEEECCCCCC
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITL
CHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEE
HGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDAS
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCH
AASLLCKLGFNAVHHPAR
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKILVDENMPYARELFSRLGEVKAVPGRPIPVVQLDDADALMVRSVTKVNESLLAGKPIK
CEEEECCCCCHHHHHHHHHCCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEE
FVGTATAGTDHVDEVWLKQAGIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHERTVG
EEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCEEE
IVGVGNVGRRLQARLEVLGIKTLLCDPPRADRGDEGDFRSLDELVQHADILTFHTPLFKD
EEECCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
GPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
CCCCEEEHHHHHHHHHCCCCEEEEECCCCCEECCCEEEEEECCCCEEEEEEEEECCCCCC
NVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITL
CHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEE
HGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDAS
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCH
AASLLCKLGFNAVHHPAR
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA