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The map label for this gene is rfaI [H]

Identifier: 157159286

GI number: 157159286

Start: 4120062

End: 4121078

Strand: Reverse

Name: rfaI [H]

Synonym: EcE24377A_4130

Alternate gene names: 157159286

Gene position: 4121078-4120062 (Counterclockwise)

Preceding gene: 157158623

Following gene: 157154988

Centisome position: 82.76

GC content: 38.15

Gene sequence:

>1017_bases
ATGAGTGCCCACTATTTTAATCCACAAGAGATGATCAATAAGACAATCATCTTCGATGAAAGGCCAGCGGCGTCAGTGGC
ATCATCATTCCATGTTGCTTATGGCATTGATAAAAACTTTCTTTTTGGTTGTGGTGTTTCAATCACGTCAGTTTTGTTAC
ATAACAACGACGTGAGTTTTGTTTTCCACGTTTTTATTGATGATATCCCTGAAGCCGATATCCAGCGTTTAGCCCAATTG
GCGAAAAGCTATCGTACCTGTATCCAGATCCATCTAGTAAATTGTGAACGGCTTAAGGCATTACCGACGACCAAAAATTG
GTCTATTGCCATGTATTTCCGTTTTGTAATTGCAGATTACTTTATTGATCAACAAGATAAGATCCTTTACCTGGATGCTG
ATATCGCCTGTCAGGGAAACTTAAAGCCGCTGATAACAATGGATCTTGCCAATAACGTTGCTGCTGTTGTTACTGAACGC
GATGCTAACTGGTGGTCGTTACGGGGTCAAAGTCTGCAGTGTAATGAACTTGAAAAGGGTTACTTTAATTCAGGTGTCCT
GTTAATTAATACACTAGCGTGGGCGCAGGAGTCCGTTTCTGCTAAAGCGATGTCGATGCTTGCTGATAAAGCCATCGTTT
CCCGTTTAACCTATATGGATCAAGATATCCTTAATCTTATCCTGTTAGGGAAAGTTAAATTCATTGATGCTAAATACAAT
ACGCAATTTAGTTTAAATTATGAATTAAAAAAATCATTTGTTTGTCCAATTAATGATGAAACCGTATTAATTCATTATGT
CGGCCCGACAAAACCCTGGCATTACTGGGCCGGTTATCCAAGTGCGCAACCTTTTATCAAAGCCAAAGAAGCATCGCCCT
GGAAAAATGAACCGTTAATGCGGCCAGTTAACTCAAACTATGCTCGTTATTGCGCCAAGCATAATTTTAAACAAAACAAA
CCAATTAACGGGATAATGAATTATATTTATTATTTTTATTTAAAGATAATAAAATGA

Upstream 100 bases:

>100_bases
TCTCAAGCAGGAACAAGGACTGCTGTCGCAAGCAGAAGCAAAAGCCACAAAAATCAGGGAAAGAACGATTCGAAAATCGT
TGTAATTTCCGGAGAGAAAT

Downstream 100 bases:

>100_bases
AATTGAGAAAAGTTAAATGAATGAATTTATAAAAGAACGGTTTTCGTATTTAGCAGATAATAAAAAAGAAAACGCCCCAG
AGCTAAATGTTTCCTACGGT

Product: lipopolysaccharide 1,3-galactosyltransferase

Products: NA

Alternate protein names: Lipopolysaccharide 3-alpha-galactosyltransferase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGCGVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQL
AKSYRTCIQIHLVNCERLKALPTTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTYMDQDILNLILLGKVKFIDAKYN
TQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYWAGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNK
PINGIMNYIYYFYLKIIK

Sequences:

>Translated_338_residues
MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGCGVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQL
AKSYRTCIQIHLVNCERLKALPTTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTYMDQDILNLILLGKVKFIDAKYN
TQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYWAGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNK
PINGIMNYIYYFYLKIIK
>Mature_337_residues
SAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGCGVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLA
KSYRTCIQIHLVNCERLKALPTTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTERD
ANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTYMDQDILNLILLGKVKFIDAKYNT
QFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYWAGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKP
INGIMNYIYYFYLKIIK

Specific function: Adds the galactose(I) group on the glucose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790057, Length=338, Percent_Identity=50.5917159763314, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1790056, Length=333, Percent_Identity=36.6366366366366, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.44 [H]

Molecular weight: Translated: 38797; Mature: 38666

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGCGVSITSVLLHNNDVSF
CCCCCCCHHHHHCCEEEECCCCHHHHHHCEEEEEECCCCCEEECCHHHEEEEEECCCCEE
VFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALPTTKNWSIAMYFRFVIADY
EEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHH
FIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTERDANWWSLRGQSLQCNELEKG
HCCCCCCEEEEECCEEECCCCCEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCC
YFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTYMDQDILNLILLGKVKFIDAKYN
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
TQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYWAGYPSAQPFIKAKEASPWKNEPLM
CEEEEEEEECCCEECCCCCCEEEEEECCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCC
RPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYLKIIK
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGCGVSITSVLLHNNDVSF
CCCCCCHHHHHCCEEEECCCCHHHHHHCEEEEEECCCCCEEECCHHHEEEEEECCCCEE
VFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALPTTKNWSIAMYFRFVIADY
EEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHH
FIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTERDANWWSLRGQSLQCNELEKG
HCCCCCCEEEEECCEEECCCCCEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCC
YFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTYMDQDILNLILLGKVKFIDAKYN
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
TQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYWAGYPSAQPFIKAKEASPWKNEPLM
CEEEEEEEECCCEECCCCCCEEEEEECCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCC
RPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYLKIIK
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]