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The map label for this gene is rfaP

Identifier: 157158623

GI number: 157158623

Start: 4121094

End: 4121891

Strand: Reverse

Name: rfaP

Synonym: EcE24377A_4131

Alternate gene names: 157158623

Gene position: 4121891-4121094 (Counterclockwise)

Preceding gene: 157157470

Following gene: 157159286

Centisome position: 82.78

GC content: 47.37

Gene sequence:

>798_bases
ATGGTTGAACTTAAAGAGCCGTTTGCCACGTTATGGCGCGGCAAAGATCCTTTTGAGGAAGTTAAAACCTTGCAGGGTGA
GGTATTTCGTGAACTGGAAACTCGCCGTACTCTGCGCTTTGAAATGGCGGGCAAAAGCTATTTTCTCAAATGGCATCGCG
GCACGACCCTGAAAGAGATAATCAAAAATTTACTCTCATTGCGGATGCCAGTATTAGGCGCAGACCGCGAATGGAATGCG
ATTCATCGACTGCGGGATGTCGGCGTTGATACTATGTATGGGGTGGCATTCGGCGAAAAAGGCATGAATCCGCTGACCAG
AACCTCGTTTATTATTACCGAAGATCTGACACCAACCATAAGTCTGGAAGATTACTGTGCTGATTGGGCGACTAACCCTC
CAGATGTTCGTGTAAAGCGTATGCTTATTAAGCGTGTCGCGACGATGGTGCGCGATATGCATGCTGCGGGCATTAACCAC
CGTGACTGTTATATCTGTCATTTCCTGCTGCACTTGCCTTTTTCCGGTAAGGAAGAGGAGTTAAAAATTTCGGTAATTGA
CCTACACCGGGCGCAGCTTCGCACGCGCGTTCCACGTCGTTGGCGCGATAAAGATCTTATAGGGCTTTATTTTTCTTCAA
TGAATATCGGCCTGACTCAGCGGGATATCTGGCGGTTTATGAAAGTGTATTTTGCCGCCCCGCTTAAAGACATTCTCAAG
CAGGAACAAGGACTGCTGTCGCAAGCAGAAGCAAAAGCCACAAAAATCAGGGAAAGAACGATTCGAAAATCGTTGTAA

Upstream 100 bases:

>100_bases
TTGCGCCAGGCCTGGGCGGAAAATGCGCGACATTATGCTGATACACAAGATTTATACAGTCTGCCAGAGAAAGCGGCGGA
TATCATAACGGGTGGTCTGG

Downstream 100 bases:

>100_bases
TTTCCGGAGAGAAATATGAGTGCCCACTATTTTAATCCACAAGAGATGATCAATAAGACAATCATCTTCGATGAAAGGCC
AGCGGCGTCAGTGGCATCAT

Product: lipopolysaccharide core biosynthesis protein RfaP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNA
IHRLRDVGVDTMYGVAFGEKGMNPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFAAPLKDILK
QEQGLLSQAEAKATKIRERTIRKSL

Sequences:

>Translated_265_residues
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNA
IHRLRDVGVDTMYGVAFGEKGMNPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFAAPLKDILK
QEQGLLSQAEAKATKIRERTIRKSL
>Mature_265_residues
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNA
IHRLRDVGVDTMYGVAFGEKGMNPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFAAPLKDILK
QEQGLLSQAEAKATKIRERTIRKSL

Specific function: Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. KdkA/rfaP family

Homologues:

Organism=Escherichia coli, GI1790060, Length=265, Percent_Identity=84.1509433962264, Blast_Score=473, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFAP_ECOLX (Q9R9D6)

Other databases:

- EMBL:   AF019746
- GO:   GO:0016020
- InterPro:   IPR017172
- InterPro:   IPR010440
- PIRSF:   PIRSF037318

Pfam domain/function: PF06293 Kdo

EC number: 2.7.-.-

Molecular weight: Translated: 31052; Mature: 31052

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEI
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCHHHHH
IKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGMNPLTRTSFIITEDLTPTI
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHEEEEEECCCCCC
SLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINHRDCYICHFLLHLPFSGKEEE
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
LKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFAAPLKDILK
EEEEEEHHHHHHHHHHCCHHCCCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QEQGLLSQAEAKATKIRERTIRKSL
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEI
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCHHHHH
IKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGMNPLTRTSFIITEDLTPTI
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHEEEEEECCCCCC
SLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINHRDCYICHFLLHLPFSGKEEE
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
LKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFAAPLKDILK
EEEEEEHHHHHHHHHHCCHHCCCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QEQGLLSQAEAKATKIRERTIRKSL
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA