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The map label for this gene is treA

Identifier: 157159159

GI number: 157159159

Start: 1336361

End: 1338058

Strand: Reverse

Name: treA

Synonym: EcE24377A_1343

Alternate gene names: 157159159

Gene position: 1338058-1336361 (Counterclockwise)

Preceding gene: 157157322

Following gene: 157157294

Centisome position: 26.87

GC content: 52.12

Gene sequence:

>1698_bases
ATGAAATCCCCCGCACCTTCTCGCCCGCAAAAAATGGCGTTAATTCCAGCCTGTATCTTTTTGTGTTTCGCTGCGCTATC
GGTGCAGGCAGAAGAAACACCGGTAACACCACAGCCGCCTGATATTTTATTAGGGCCGCTGTTTAATGATGTGCAAAACG
CCAAACTTTTTCCGGACCAAAAAACCTTTGCCGATGCCGTGCCGAACAGCGATCCGCTGATGATCCTTGCTGATTATCGG
ATGCAGCAAAACCAGAGCGGATTTGATCTGCGCCATTTCGTTAACGTCAATTTCACCCTGCCGAAAGAAGGCGAGAAATA
TGTTCCGCCAGAGGGGCAGTCACTGCGCGAACATATTGACGGACTTTGGCCGGTATTAACGCGTTCTACCGAAAACACAG
AAAAATGGGATTCTCTGTTACCGCTGCCGGAACCTTATGTCGTGCCGGGCGGACGTTTTCGCGAGGTATATTACTGGGAC
AGTTACTTCACCATGTTAGGACTTGCCGAAAGCGGTCACTGGGATAAAGTCGCGGATATGGTGGCCAATTTTGCTCATGA
AATAGACACTTACGGTCATATTCCCAACGGCAACCGCAGTTACTATTTAAGCCGCTCGCAACCGCCCTTCTTTGCCATGA
TGGTAGAGTTACTGGCGCAGCATGAAGGCGATGCCGCGTTGAAGCAATACCTGCCGCAAATGCAAAAAGAATATGCTTAC
TGGATGGACGGTGTTGAAAACCTGCAAGCCGGACAACAGGAAAAACGCGTTGTCAAACTTCAGGATGGTACCCTTCTCAA
CCGCTACTGGGACGATCGCGATACGCCACGACCAGAGTCATGGGTGGAAGATATTGCCACCGCCAAAAGCAATCCGAATC
GACCTGCCACTGAAATTTACCGCGACCTGCGCTCTGCCGCTGCGTCTGGCTGGGATTTCAGCTCGCGCTGGATGGACAAC
CCGCAGCAGTTAAATACCTTACGCACCACCAGCATCGTACCGGTCGATCTGAACAGCCTGATGTTTAAAATGGAAAAAAT
CCTCGCCCGCGCCAGCAAAGCTGCCGGAGATAACGCGATGGCAAACCAGTACGAAACGCTGGCAAATGCCCGTCAAAAAG
GGATCGAAAAATACCTGTGGAACGATCAACAAGGCTGGTATGCCGATTACGACCTGAAAAGTCATAAAGTGCGCAATCAG
TTAACCGCGGCCGCCCTGTTCCCGCTGTACGTCAATGCGGCAGCGAAAGATCGCGCCAACAAAATGGCGACGGCGACGAA
AACACATCTGCTGCAACCCGGCGGCCTGAACACCACGTCGGTGAAAAGTGGGCAACAATGGGATGCGCCAAATGGCTGGG
CACCGTTACAGTGGGTCGCGACAGAAGGATTACAAAACTACGGGCAAAAAGAGGTGGCGATGGACATTAGCTGGCACTTC
CTGACCAATGTTCAGCACACCTATGACCGGGAGAAAAAGCTGGTGGAAAAATATGATGTCAGCACCACCGGAACGGGGGG
CGGCGGTGGCGAATATCCATTACAGGATGGCTTTGGCTGGACCAATGGCGTGACGCTGAAAATGCTGGATTTGATCTGCC
CGAAAGAGCAACCGTGTGACAATGTTCCGGCGACGCGTCCGACCGTTAAGTCAGCAACGACGCAACCCTCAACCAAAGAG
GCACAACCCACACCTTAA

Upstream 100 bases:

>100_bases
GCAGAATGAGATTTCGATCATGCAGCTAGTGCGATCCTGAACTAAGGTTTTCTGATACTTGAATACCGTTTTATTCGGTT
TCGCCAAAGGAGAATGATTG

Downstream 100 bases:

>100_bases
CCAGCGCTTACTCCGACAACGTCATTCGGCTTCTCCGGATGGCGTTGTCGGCAGATAACACCATAGCTATGGTGTTTTCT
ACACAGACGGTTTTTCAAGT

Product: trehalase

Products: NA

Alternate protein names: Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYR
MQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFAMMVELLAQHEGDAALKQYLPQMQKEYAY
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDN
PQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQ
LTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKE
AQPTP

Sequences:

>Translated_565_residues
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYR
MQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFAMMVELLAQHEGDAALKQYLPQMQKEYAY
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDN
PQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQ
LTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKE
AQPTP
>Mature_565_residues
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYR
MQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFAMMVELLAQHEGDAALKQYLPQMQKEYAY
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDN
PQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQ
LTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKE
AQPTP

Specific function: Provides the cells with the ability to utilize trehalose at high osmolarity by splitting it into glucose molecules that can subsequently be taken up by the phosphotransferase-mediated uptake system

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 37 family

Homologues:

Organism=Homo sapiens, GI116284412, Length=526, Percent_Identity=33.6501901140684, Blast_Score=264, Evalue=1e-70,
Organism=Escherichia coli, GI1787447, Length=565, Percent_Identity=99.8230088495575, Blast_Score=1177, Evalue=0.0,
Organism=Escherichia coli, GI1789936, Length=487, Percent_Identity=50.9240246406571, Blast_Score=512, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17542196, Length=520, Percent_Identity=34.0384615384615, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI25148109, Length=574, Percent_Identity=31.7073170731707, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI25141398, Length=527, Percent_Identity=31.6888045540797, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17565078, Length=545, Percent_Identity=31.5596330275229, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI71987755, Length=403, Percent_Identity=29.0322580645161, Blast_Score=184, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320204, Length=436, Percent_Identity=30.045871559633, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6319473, Length=428, Percent_Identity=30.3738317757009, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24656680, Length=528, Percent_Identity=34.280303030303, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24656675, Length=528, Percent_Identity=34.280303030303, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24656661, Length=528, Percent_Identity=34.280303030303, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI17933716, Length=528, Percent_Identity=34.280303030303, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24656670, Length=528, Percent_Identity=34.280303030303, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24656685, Length=502, Percent_Identity=33.6653386454183, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI22024178, Length=527, Percent_Identity=31.3092979127135, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI45551104, Length=367, Percent_Identity=32.6975476839237, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28573474, Length=314, Percent_Identity=28.6624203821656, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TREA_ECO24 (A7ZKW9)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462448.1
- ProteinModelPortal:   A7ZKW9
- SMR:   A7ZKW9
- STRING:   A7ZKW9
- EnsemblBacteria:   EBESCT00000019345
- GeneID:   5586340
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1343
- NMPDR:   fig|331111.3.peg.3804
- eggNOG:   COG1626
- GeneTree:   EBGT00050000010574
- HOGENOM:   HBG485982
- OMA:   NRYWDAS
- ProtClustDB:   PRK13271
- BioCyc:   ECOL331111:ECE24377A_1343-MONOMER
- HAMAP:   MF_01060
- InterPro:   IPR008928
- InterPro:   IPR001661
- InterPro:   IPR018232
- PANTHER:   PTHR23403
- PRINTS:   PR00744

Pfam domain/function: PF01204 Trehalase; SSF48208 Glyco_trans_6hp

EC number: =3.2.1.28

Molecular weight: Translated: 63655; Mature: 63655

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00927 TREHALASE_1; PS00928 TREHALASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCH
KTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHID
HHHHHHCCCCCCEEEEECCHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
GLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWDSYFTMLGLAESGHWDKVADM
HHHHHHHCCCCCHHHHHHCCCCCCCEECCCCCEEEEEEECHHHHHHHCCCCCCHHHHHHH
VANFAHEIDTYGHIPNGNRSYYLSRSQPPFFAMMVELLAQHEGDAALKQYLPQMQKEYAY
HHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIY
HHHHHHHHHCCCHHCCEEEECCCEEHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
RDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAM
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHCCCCHH
ANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQLTAAALFPLYVNAAAKDRAN
HHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHH
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCD
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
NVPATRPTVKSATTQPSTKEAQPTP
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCH
KTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHID
HHHHHHCCCCCCEEEEECCHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
GLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWDSYFTMLGLAESGHWDKVADM
HHHHHHHCCCCCHHHHHHCCCCCCCEECCCCCEEEEEEECHHHHHHHCCCCCCHHHHHHH
VANFAHEIDTYGHIPNGNRSYYLSRSQPPFFAMMVELLAQHEGDAALKQYLPQMQKEYAY
HHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIY
HHHHHHHHHCCCHHCCEEEECCCEEHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
RDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAM
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHCCCCHH
ANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQLTAAALFPLYVNAAAKDRAN
HHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHH
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCD
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
NVPATRPTVKSATTQPSTKEAQPTP
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA