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The map label for this gene is dhaM [H]

Identifier: 157157322

GI number: 157157322

Start: 1338378

End: 1339796

Strand: Reverse

Name: dhaM [H]

Synonym: EcE24377A_1344

Alternate gene names: 157157322

Gene position: 1339796-1338378 (Counterclockwise)

Preceding gene: 157155726

Following gene: 157159159

Centisome position: 26.91

GC content: 49.82

Gene sequence:

>1419_bases
ATGGTAAACCTGGTCATAGTTTCACATAGCAGCCGACTGGGAGAAGGTGTCGGTGAATTAGCCCGTCAGATGTTAATGAG
TGATAGTTGTAAAATCGCCATTGCCGCGGGAATTGACGATCCACAAAATCCTATTGGTACCGATGCCGTCAAAGTGATGG
AGGCCATCGAATCTGTTGCTGATGCCGACCATGTGCTGGTCATGATGGATATGGGTAGCGCATTATTGAGTGCTGAAACT
GCGCTGGAATTGCTGGCTCCCAAGATCGCCGCAAAAGTACGTTTGTGTGCTGCGCCGTTGGTCGAAGGTACACTGGCAGC
AACGGTCAGCGCGGCCTCGGGAGCGGATATCGACAAAGTTATCTTTGACGCTATGCATGCGCTGGAAGCCAAACGTGAAC
AACTGGGTTTACCGTCCTCCGACACTAAAATCTCTGACACATGTCCTGCGTACGATGAAGAAGCCCGTTCTCTGGCGGTG
GTCATAAAAAACCGTAACGGCCTGCATGTACGTCCGGCCTCCCGGCTGGTTTATACCTTATCGACATTTAATGCCGATAT
GTTGCTGGAAAAAAACGGCAAATGCGTCACACCAGAGAGTATTAACCAGATTGCGTTACTACAAGTTCGCTATAACGATA
CGCTGCGCCTGATTGCGAAAGGGCCAGAAGCTGAAGAGGCACTGATCGCTTTCCGTCAGCTGGCTGAAGATAACTTTGGT
GAAACGGAGGAAGTCGCTCCACCTACTCTGCGTCCCGTTCCGCCTGTTTCGGGTAAAGCCTTTTATTATCAACCAGTTTT
ATGTACGGTACAGGCAAAATCAACCCTGACCGTGGAAGAAGAACAAGATCGATTACGCCAGGCTATTGACTTCACGTTAT
TAGATCTGATGACGTTAACAGCGAAAGCAGAAGCCAGCGGGCTTGACGATATTGCCGCAATCTTTTCTGGTCACCATACA
CTGTTAGATGATCCGGAACTGCTGGCGGCGGCAAGCGAACTCCTTCAGCATGAACATTGCACGGCAGAATATGCCTGGCA
GCAAGTTCTTAAAGAACTTAGCCAGCAATACCAGCAACTGGATGATGAATATCTACAAGCTCGCTATATTGATGTGGACG
ATCTTCTGCATCGCACCCTGGTCCACCTGACCCAAACGAAAGAAGAACTCCCGCAGTTTAACTCGCCAACTATTCTACTG
GCGGAGAACATTTATCCTTCCACAGTACTGCAACTGGATCCGGCGGTTGTAAAAGGTATCTGCCTTAGCGCCGGAAGTCC
GGTATCCCACAGCGCCCTAATCGCCCGTGAACTGGGGATTGGCTGGATTTGCCAGCAGGGTGAGAAACTGTATGCGATAC
AACCAGAAGAAACGCTAACGCTGGACGTTAAAAGGCAACGTTTCAACCGTCAGGGTTAA

Upstream 100 bases:

>100_bases
CTCGGTGAACGCAGTATTGGTCACCAGGATCCCGGCGCGACCTCGGTGATGTTTATGATGCAAATGTTGGCGTTAGCCGC
AAAAGAGTAAGGAATTGGTG

Downstream 100 bases:

>100_bases
GCCACTGTTCGGATGGCATCGTTCTGATGTCATCCGGCAATTTACTCAGCACTAATGGTTTTTAACTACCTGTTATATTT
TTCACCAATTTATGATGATT

Product: dihydroxyacetone kinase subunit M

Products: NA

Alternate protein names: Phosphocarrier protein HPr; Protein H; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAET
ALELLAPKIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHT
LLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILL
AENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKRQRFNRQG

Sequences:

>Translated_472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAET
ALELLAPKIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHT
LLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILL
AENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKRQRFNRQG
>Mature_472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAET
ALELLAPKIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHT
LLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILL
AENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKRQRFNRQG

Specific function: Phosphotransferase subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaM serves as the phosphoryl donor. It is phosphorylated by HPr, then it donates its phosphoryl group to dhaL-ADP, which eventually tran

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI226510935, Length=472, Percent_Identity=99.364406779661, Blast_Score=959, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=168, Percent_Identity=32.7380952380952, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789193, Length=178, Percent_Identity=28.0898876404494, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788726, Length=298, Percent_Identity=25.8389261744966, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012844
- InterPro:   IPR008279
- InterPro:   IPR004701
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731 [H]

Pfam domain/function: PF03610 EIIA-man; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 51502; Mature: 51502

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00369 PTS_HPR_HIS ; PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVEGTLAATVSAASGADIDKV
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCHHHH
IFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAVVIKNRNGLHVRPASRLVYTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCCEECCHHHEEEEE
STFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ECCCCEEEEECCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCC
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQDRLRQAIDFTLLDLMTLT
CCCCCCCCCCCCCCCCCCCEEEEECEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AKAEASGLDDIAAIFSGHHTLLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQL
HHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILLAENIYPSTVLQLDPAVVKGI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHEEECHHHHHHH
CLSAGSPVSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKRQRFNRQG
HHCCCCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEEEHHHHCCCCC
>Mature Secondary Structure
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVEGTLAATVSAASGADIDKV
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCHHHH
IFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAVVIKNRNGLHVRPASRLVYTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCCEECCHHHEEEEE
STFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ECCCCEEEEECCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCC
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQDRLRQAIDFTLLDLMTLT
CCCCCCCCCCCCCCCCCCCEEEEECEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AKAEASGLDDIAAIFSGHHTLLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQL
HHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILLAENIYPSTVLQLDPAVVKGI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHEEECHHHHHHH
CLSAGSPVSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKRQRFNRQG
HHCCCCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2671658; 7984428 [H]