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The map label for this gene is outD [H]

Identifier: 157159048

GI number: 157159048

Start: 1701981

End: 1703201

Strand: Reverse

Name: outD [H]

Synonym: EcE24377A_1698

Alternate gene names: 157159048

Gene position: 1703201-1701981 (Counterclockwise)

Preceding gene: 157158448

Following gene: 157157168

Centisome position: 34.2

GC content: 54.71

Gene sequence:

>1221_bases
ATGAATATCAGAAAATTACTTCTCCCGGTGCTGCCTGCAATGGCGCTGTTATTCCCGCTGCCGTCGTTTTCAGCCGGGAC
GGAACTGGATATTAACCGCATGAGCCTGCCTGATGCGGTGACGCTGCTCTGGACGGAAGTCCTGAAAACACCGTTCATGC
TGGCCCCTGAACTGGTCAATGACCCGCGGGCCGTCACGCTGCATATTTCACCGGATATCGATGAACGGGAATTTATTACC
CGTTATCTCGGTAATATGAATATCAGGATAAGCCGTAAAAAGGGCGTTGATTATATTTACAGTCATACACCTGCCGCACC
AGAGGAGCCGCTGAAATCCCTTGTTTACACGCCCCGTTACCGGACAGTGGAGTATCTGCATCAGGCGCTTTCCGGTCTGG
GTCAGCTTCCGGCCGCACAACAGCCGGTTCAGGGGGCAAACGGTGAGCAGACATGGCAGGCGGTCAGCAGTGGTACCCGG
TTCATCAGTGCATCCGGGGATGTGTTTGTGTTCCGGGGAACGGCCCGGGAAGTTGAGCTTGTCCGGCAGCTTCTGCCGCA
GATTGACGTCAGGGCACAGGAGGTTTCGGTTGCCGGGTATGTGTTTGAGGTCCAGACCAGTGAGCGTAACGGTTCAGGTC
TGGCGCTGGCCGCAGAGCTTTTATCCGGTCGTTTCAGTATCACCATGAGCAGCGCATCGGGGCTGGATAACTTTATTCGC
CTCAGCACGGGGTCTGTTGATGCAATGTATGAGTTATTCAGGACCGACAGCCGTTTTCAGGTGGTCAGTTCGCCACGTCT
GCGCGTTATCTCCGGTAAGGAGGCGGTTTTCAGCGTGGGGTCTGATGTGCCGGTTCTGTCATCAGTGTCATGGCAGGATA
AGGTTCCGGTGCAGTCCGTGGAATACCGCAGCAGCGGGGCTATTTTTCGGGTAAAACCCACCGTGACGCAGGATGTGATA
GGTCTGGATATCGTTCAGCAGCTGAGTAACTTTGCAAAAACGGATACGGGGGTGAATAACACGCCAACGCTGATAAAGCG
CGAGGTGTCCACGTCGGTGAGTCTGAATGACGGTGATATCATTGTGCTGGGCGGACTGGCGGAGAACAAGACATCAAAGG
CGCGGACAGGGCTTTCATTTCTGCCGGATGTGTTTGGTTCAGACTCTGATGAACGTGCGAAAACCGATATCATCGTCGTT
CTTCAGGCCCGGCGGGTCTGA

Upstream 100 bases:

>100_bases
GTTTCACAGCGGGTCAGGCAGTGAACCTTCCCCGTCGCGTTCGTCCGGCAGCGGTGCCGGGGGGATGTCCGTTTCTCTCA
CCACACCATAGGGACAGAAT

Downstream 100 bases:

>100_bases
GTGTGTGATGATGTCCGTCAGGACATGTAAGGCGGCATGATGAAGTTTCATGTGACGCAGATGTCAGCCCGCCGAAACCG
GGGAGAGATGACAGAGCAGC

Product: type II/III secretion system protein

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNIRKLLLPVLPAMALLFPLPSFSAGTELDINRMSLPDAVTLLWTEVLKTPFMLAPELVNDPRAVTLHISPDIDEREFIT
RYLGNMNIRISRKKGVDYIYSHTPAAPEEPLKSLVYTPRYRTVEYLHQALSGLGQLPAAQQPVQGANGEQTWQAVSSGTR
FISASGDVFVFRGTAREVELVRQLLPQIDVRAQEVSVAGYVFEVQTSERNGSGLALAAELLSGRFSITMSSASGLDNFIR
LSTGSVDAMYELFRTDSRFQVVSSPRLRVISGKEAVFSVGSDVPVLSSVSWQDKVPVQSVEYRSSGAIFRVKPTVTQDVI
GLDIVQQLSNFAKTDTGVNNTPTLIKREVSTSVSLNDGDIIVLGGLAENKTSKARTGLSFLPDVFGSDSDERAKTDIIVV
LQARRV

Sequences:

>Translated_406_residues
MNIRKLLLPVLPAMALLFPLPSFSAGTELDINRMSLPDAVTLLWTEVLKTPFMLAPELVNDPRAVTLHISPDIDEREFIT
RYLGNMNIRISRKKGVDYIYSHTPAAPEEPLKSLVYTPRYRTVEYLHQALSGLGQLPAAQQPVQGANGEQTWQAVSSGTR
FISASGDVFVFRGTAREVELVRQLLPQIDVRAQEVSVAGYVFEVQTSERNGSGLALAAELLSGRFSITMSSASGLDNFIR
LSTGSVDAMYELFRTDSRFQVVSSPRLRVISGKEAVFSVGSDVPVLSSVSWQDKVPVQSVEYRSSGAIFRVKPTVTQDVI
GLDIVQQLSNFAKTDTGVNNTPTLIKREVSTSVSLNDGDIIVLGGLAENKTSKARTGLSFLPDVFGSDSDERAKTDIIVV
LQARRV
>Mature_406_residues
MNIRKLLLPVLPAMALLFPLPSFSAGTELDINRMSLPDAVTLLWTEVLKTPFMLAPELVNDPRAVTLHISPDIDEREFIT
RYLGNMNIRISRKKGVDYIYSHTPAAPEEPLKSLVYTPRYRTVEYLHQALSGLGQLPAAQQPVQGANGEQTWQAVSSGTR
FISASGDVFVFRGTAREVELVRQLLPQIDVRAQEVSVAGYVFEVQTSERNGSGLALAAELLSGRFSITMSSASGLDNFIR
LSTGSVDAMYELFRTDSRFQVVSSPRLRVISGKEAVFSVGSDVPVLSSVSWQDKVPVQSVEYRSSGAIFRVKPTVTQDVI
GLDIVQQLSNFAKTDTGVNNTPTLIKREVSTSVSLNDGDIIVLGGLAENKTSKARTGLSFLPDVFGSDSDERAKTDIIVV
LQARRV

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 44422; Mature: 44422

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRKLLLPVLPAMALLFPLPSFSAGTELDINRMSLPDAVTLLWTEVLKTPFMLAPELVN
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHCC
DPRAVTLHISPDIDEREFITRYLGNMNIRISRKKGVDYIYSHTPAAPEEPLKSLVYTPRY
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHCCCCH
RTVEYLHQALSGLGQLPAAQQPVQGANGEQTWQAVSSGTRFISASGDVFVFRGTAREVEL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCEEEEECCHHHHHH
VRQLLPQIDVRAQEVSVAGYVFEVQTSERNGSGLALAAELLSGRFSITMSSASGLDNFIR
HHHHHHHCCCCHHEEEEEEEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCEEE
LSTGSVDAMYELFRTDSRFQVVSSPRLRVISGKEAVFSVGSDVPVLSSVSWQDKVPVQSV
ECCCCHHHHHHHHHCCCCEEEECCCCEEEEECCHHEEECCCCCCCCCCCCCCCCCCCHHE
EYRSSGAIFRVKPTVTQDVIGLDIVQQLSNFAKTDTGVNNTPTLIKREVSTSVSLNDGDI
EECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEHHCCEEEECCCCE
IVLGGLAENKTSKARTGLSFLPDVFGSDSDERAKTDIIVVLQARRV
EEECCCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MNIRKLLLPVLPAMALLFPLPSFSAGTELDINRMSLPDAVTLLWTEVLKTPFMLAPELVN
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHCC
DPRAVTLHISPDIDEREFITRYLGNMNIRISRKKGVDYIYSHTPAAPEEPLKSLVYTPRY
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHCCCCH
RTVEYLHQALSGLGQLPAAQQPVQGANGEQTWQAVSSGTRFISASGDVFVFRGTAREVEL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCEEEEECCHHHHHH
VRQLLPQIDVRAQEVSVAGYVFEVQTSERNGSGLALAAELLSGRFSITMSSASGLDNFIR
HHHHHHHCCCCHHEEEEEEEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCEEE
LSTGSVDAMYELFRTDSRFQVVSSPRLRVISGKEAVFSVGSDVPVLSSVSWQDKVPVQSV
ECCCCHHHHHHHHHCCCCEEEECCCCEEEEECCHHEEECCCCCCCCCCCCCCCCCCCHHE
EYRSSGAIFRVKPTVTQDVIGLDIVQQLSNFAKTDTGVNNTPTLIKREVSTSVSLNDGDI
EECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEHHCCEEEECCCCE
IVLGGLAENKTSKARTGLSFLPDVFGSDSDERAKTDIIVVLQARRV
EEECCCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]