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The map label for this gene is rfaG [H]

Identifier: 157157470

GI number: 157157470

Start: 4121884

End: 4123008

Strand: Reverse

Name: rfaG [H]

Synonym: EcE24377A_4132

Alternate gene names: 157157470

Gene position: 4123008-4121884 (Counterclockwise)

Preceding gene: 157157104

Following gene: 157158623

Centisome position: 82.8

GC content: 48.36

Gene sequence:

>1125_bases
ATGATCGTTGCTTTTTGTTTATATAAATATTTTCCCTTTGGCGGTTTGCAGCGCGATTTTATGCGTATTGCTCAGACAGT
CGCCGCCCGAGGTCATCATGTTCGGGTTTATACCCAGTCGTGGGAAGGCGAATGCCCTGATGTATTTGAACTGATCAAAG
TGCCGGTTAAATCGCGTACCAATCACGGGCGCAATGCGGAGTATTTTACCTGGGTGCAAAAACATTTACGCGAACATCCC
GTCGATAAAGTCGTTGGATTCAACAAAATGCCGGGGCTGGACGTTTATTATGCCGCTGATGTTTGTTATGCCGAGAAAGT
AGCGCAGGAGAAAGGCTTTTTCTATCGCCTGACGTCACGTTATCGCCATTATGCCGCCTTTGAGCGGGCAACCTTCGAAC
AGGGCAAGCCGACACAGCTGCTGATGCTGACAGATAAGCAAATCGCCGATTTCCAGAAACATTATCAGACTGAAGCGGAG
CGTTTTCATATTCTGCCACCGGGGATTTATCCTGATCGTAAATATAGCCAGCAGCCAGCCAATAGCCGTGAAATATTCCG
TAAGAAGAATGGAATAACCGAACAACAATATTTATTGTTGCAGGTCGGTTCAGACTTCACGCGTAAAGGTGTCGATCGTT
CCATTGAAGCACTTGCTTCGTTACCGGATTCGCTGCGCCACAACACATTGCTATATGTTGTTGGGCAGGATAAACCGCGA
AAATTTGAGGCGCTGGCAGAAAAACGCGGCGTGCGCAGTAATGTTCACTTTTTCTCGGGGCGCAATGATGTCTCGGAATT
AATGGCGGCGGCGGATTTATTACTGCATCCTGCCTACCAGGAAGCGGCGGGAATTGTGCTGCTGGAAGCGATTACTGCAG
GATTACCGGTACTAACAACAGCCGTTTGTGGCTATGCGCATTATATTGTCGACGCCAATTGCGGCGAGGCTATTGCTGAG
CCATTCCGCCAGGAAACATTGAATGAGATTTTACGCAAAGCGTTAACGCAATCTTCATTGCGCCAGGCCTGGGCGGAAAA
TGCGCGACATTATGCTGATACACAAGATTTATACAGTCTGCCAGAGAAAGCGGCGGATATCATAACGGGTGGTCTGGATG
GTTGA

Upstream 100 bases:

>100_bases
AGCGTGACCGAAATGAGATGTATCTTTCGGTTATTCCGGCGGCAGATGTCATTGCTGCTGTCGATAAATTACTGCCCTCC
TCCACGACAGGTACGTCGTT

Downstream 100 bases:

>100_bases
ACTTAAAGAGCCGTTTGCCACGTTATGGCGCGGCAAAGATCCTTTTGAGGAAGTTAAAACCTTGCAGGGTGAGGTATTTC
GTGAACTGGAAACTCGCCGT

Product: lipopolysaccharide core biosynthesis protein RfaG

Products: NA

Alternate protein names: Glucosyltransferase I [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGECPDVFELIKVPVKSRTNHGRNAEYFTWVQKHLREHP
VDKVVGFNKMPGLDVYYAADVCYAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEALASLPDSLRHNTLLYVVGQDKPR
KFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLHPAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAE
PFRQETLNEILRKALTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG

Sequences:

>Translated_374_residues
MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGECPDVFELIKVPVKSRTNHGRNAEYFTWVQKHLREHP
VDKVVGFNKMPGLDVYYAADVCYAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEALASLPDSLRHNTLLYVVGQDKPR
KFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLHPAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAE
PFRQETLNEILRKALTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG
>Mature_374_residues
MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGECPDVFELIKVPVKSRTNHGRNAEYFTWVQKHLREHP
VDKVVGFNKMPGLDVYYAADVCYAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEALASLPDSLRHNTLLYVVGQDKPR
KFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLHPAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAE
PFRQETLNEILRKALTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG

Specific function: Involved in the addition of the first glucose residue to the lipopolysaccharide core [H]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790061, Length=374, Percent_Identity=89.8395721925134, Blast_Score=709, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 42578; Mature: 42578

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGECPDVFELIKVPVKSRT
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCHHHCC
NHGRNAEYFTWVQKHLREHPVDKVVGFNKMPGLDVYYAADVCYAEKVAQEKGFFYRLTSR
CCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHH
YRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAERFHILPPGIYPDRKYSQQPA
HHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCC
NSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEALASLPDSLRHNTLLYVVGQDKPR
CHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHCHHHHCCCEEEEEEECCCCH
KFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLHPAYQEAAGIVLLEAITAGLPVLTT
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
AVCGYAHYIVDANCGEAIAEPFRQETLNEILRKALTQSSLRQAWAENARHYADTQDLYSL
HHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
PEKAADIITGGLDG
HHHHHHHHCCCCCC
>Mature Secondary Structure
MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGECPDVFELIKVPVKSRT
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCHHHCC
NHGRNAEYFTWVQKHLREHPVDKVVGFNKMPGLDVYYAADVCYAEKVAQEKGFFYRLTSR
CCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHH
YRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAERFHILPPGIYPDRKYSQQPA
HHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCC
NSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEALASLPDSLRHNTLLYVVGQDKPR
CHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHCHHHHCCCEEEEEEECCCCH
KFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLHPAYQEAAGIVLLEAITAGLPVLTT
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
AVCGYAHYIVDANCGEAIAEPFRQETLNEILRKALTQSSLRQAWAENARHYADTQDLYSL
HHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
PEKAADIITGGLDG
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732225; 8041620; 9278503; 1447141; 7988890 [H]