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The map label for this gene is est [H]

Identifier: 157155311

GI number: 157155311

Start: 4403565

End: 4404473

Strand: Reverse

Name: est [H]

Synonym: EcE24377A_4412

Alternate gene names: 157155311

Gene position: 4404473-4403565 (Counterclockwise)

Preceding gene: 157157248

Following gene: 157157646

Centisome position: 88.45

GC content: 52.7

Gene sequence:

>909_bases
ATGGCACTGGAAAAAGGAATTGCAGAGTTAGTCGAAAAGTTTATCGCTGCGGGTCGCCCCTCATCGCGTCAACAAAGTAT
TGCGCAGCGCAGGGAGGGCTATATCGCCAGCGCTGTCCTTGCGGGTGAGACAGAAACGCGGGTAGATATTCAGACTATCG
AACTTGAAGGGATGACCTTACGAATAGTGTCGCCGCTGAATGCCCCGACATTACTCCCGACGATAATTTATTACTACGGT
GGCTGTTTTGTTAGCGGCGGGTTCGCAACTCACGACAACCAGCTGCGCCAGTTAGCCTATTACGGGCAATGTCGGGTTAT
TGCCGTCCAGTATCGGCTGGCACCGGAACATACTTTCCCCGCCGCCCATGATGATGCGCAACGAGGTGCAGAAATAGTCT
GGCAACACGCCGAACGACTCGGCGTTGATAAACAACGTATAACTCTTGCCGGTGACAGTGCGGGCGGACATCTGGCGCTG
GTAACTGCTTTGCGATTAAAGCGTGCTGGCGAGTGGCAACCGGCACAACTCATCTTAATTTATCCTATGCTTGATGCGAC
TGCGCATTTCGAAAGCTACATACGCAATGGTCACGATTACATCATCACACGCGACACTTTACTGAGCGGCTTTGAGATGT
ATCTGCCAGGCATTGAACGCAGGCATCCTGAAGCCAGCCCTCTTTGGCGCGATGATTTCAATGGCTTGCCACCTGTACAT
ATCATTACCGCTGAATACGACCCACTTTGCGATGAAGGTGAAGCGTTGTATCACCGCATGACAGGGCAAGGAGTGCAATG
CACCTGCCAACGCTACCTTGGCGTTATCCACGGATTTTTTCAGTTAGGTGGTATCAGCGAGGCCGCGCGATCGGCACTAC
GGGATGTTGCCTGGAGAGCGGGAAGGTAA

Upstream 100 bases:

>100_bases
GGCGCTTTCCAGATTTTTCACGCCCGTGAATTTCCCTGTATCCATCCAGCAAAAACGGGTGTATAAGACTGTACATTCAT
ACAGCAAAATAAGTTCTACT

Downstream 100 bases:

>100_bases
CTTCGGACCTGGGCCCTGAAAAGGTTTTCAGATTGAAAACCTTTTCAGTCAAGTAAAACGCCCCTACTCTTCTTCGTTCC
CGCCCTCTTCATACGGGCCA

Product: alpha/beta fold family hydrolase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGETETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYG
GCFVSGGFATHDNQLRQLAYYGQCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGIERRHPEASPLWRDDFNGLPPVH
IITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRDVAWRAGR

Sequences:

>Translated_302_residues
MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGETETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYG
GCFVSGGFATHDNQLRQLAYYGQCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGIERRHPEASPLWRDDFNGLPPVH
IITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRDVAWRAGR
>Mature_301_residues
ALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGETETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGG
CFVSGGFATHDNQLRQLAYYGQCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLALV
TALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGIERRHPEASPLWRDDFNGLPPVHI
ITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRDVAWRAGR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=266, Percent_Identity=27.4436090225564, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI21328446, Length=118, Percent_Identity=34.7457627118644, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786682, Length=279, Percent_Identity=31.1827956989247, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71996133, Length=351, Percent_Identity=24.2165242165242, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=26.7924528301887, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI72001146, Length=113, Percent_Identity=33.6283185840708, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540028, Length=150, Percent_Identity=30.6666666666667, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115533410, Length=137, Percent_Identity=29.9270072992701, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=137, Percent_Identity=29.9270072992701, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33607; Mature: 33475

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGETETRVDIQTIELEGMTL
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEEECCEEE
RIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYGQCRVIAVQYRLAPEHTFP
EEECCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCC
AAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLALVTALRLKRAGEWQPAQLILI
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
YPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGIERRHPEASPLWRDDFNGLPPVH
EECHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCEE
IITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRDVAWRA
EEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GR
CC
>Mature Secondary Structure 
ALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGETETRVDIQTIELEGMTL
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEEECCEEE
RIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYGQCRVIAVQYRLAPEHTFP
EEECCCCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCC
AAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLALVTALRLKRAGEWQPAQLILI
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
YPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGIERRHPEASPLWRDDFNGLPPVH
EECHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCEE
IITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRDVAWRA
EEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]