Click here to switch to the map view.

The map label for this gene is yihU [H]

Identifier: 157157646

GI number: 157157646

Start: 4397738

End: 4398634

Strand: Reverse

Name: yihU [H]

Synonym: EcE24377A_4405

Alternate gene names: 157157646

Gene position: 4398634-4397738 (Counterclockwise)

Preceding gene: 157155311

Following gene: 157158013

Centisome position: 88.33

GC content: 55.18

Gene sequence:

>897_bases
ATGGCAGCAATCGCGTTTATCGGTTTAGGACAAATGGGTTCGCCAATGGCGAGCAATTTATTGCAGCAAGGGCATCAACT
TCGCGTCTTTGATGTGAACGCCGAAGCTGTGCGGCATCTGGTAGACAAAGGTGCGACTCCCGCCGCCAACCCGGCGCAGG
CCGCTAAAGATGCCGAATTTATCATTACCATGTTGCCGAATGGCGATCTGGTGCGCAACGTGTTGTTCGGTGAAAACGGC
GTTTGCGAAGGCTTATCTACCGATGCGCTGGTCATTGATATGTCCACCATCCATCCGCTGCAAACCGATAAATTGATTGC
CGATATGCAAGCCAAAGGCTTCAGCATGATGGATGTTCCGGTAGGCCGTACTTCTGCAAATGCCATTACCGGTACTCTGT
TACTGCTGGCTGGCGGCACCGCTGAACAAGTTGAACGTGCCACACCGATCCTGATGGCGATGGGCAGTGAGTTGATCAAC
TCTGGCGGTCCGGGCATGGGGATCCGCGTTAAGCTCATCAACAACTACATGAGCATCGCGCTCAATGCGCTTTCGGCAGA
AGCCGCCGTTTTGTGCGAAGCCCTGAATCTTCCCTTCGATGTTGCCGTCAAAGTGATGAGCGGTACCGCCGCCGGTAAAG
GTCACTTCACCACTTCCTGGCCAAACAAAGTCCTCAGCGGAGATCTTTCTCCCGCCTTCATGATCGATCTTGCCCATAAG
GATCTTGGCATCGCCCTTGATGTCGCCAACCAGCTGCATGTGCCAATGCCGCTGGGGGCCGCCTCACGGGAGGTTTATAG
CCAGGCGCGCGCAGCGGGTCGCGGTCGCCAGGACTGGTCCGCCATTCTGGAACAGGTCCGTGTCAGTGCCGGGATGACTG
CCAAAGTAAAAATGTAA

Upstream 100 bases:

>100_bases
GTCAATTTTTTGACGATAAATTACAAATACGATCAAAAACAGACAAGCAAAGCATGTCATAAATGACAAAAAGTGACATC
ACTGTATTCAGGAGAGGGTT

Downstream 100 bases:

>100_bases
CGACTGGATAAAGGAATAAATGAATGAATAAGTACACCATCAACGACATTACGCGCGCATCGGGCGGTTTTGCCATGCTG
GCGGTCGATCAGCGCGAAGC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENG
VCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM

Sequences:

>Translated_298_residues
MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENG
VCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
>Mature_297_residues
AAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGV
CEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINS
GGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHKD
LGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=31.6151202749141, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=25.7142857142857, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1790315, Length=298, Percent_Identity=99.6644295302013, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=36.7857142857143, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=31.1418685121107, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789092, Length=292, Percent_Identity=29.7945205479452, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17557316, Length=283, Percent_Identity=29.6819787985866, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=33.9041095890411, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=33.9041095890411, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI28574115, Length=287, Percent_Identity=22.9965156794425, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24655240, Length=64, Percent_Identity=53.125, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 31175; Mature: 31043

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEF
CCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHCCCCEE
IITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVP
EEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECC
VGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINSGGPGMGIRVKLINNYMSIA
CCCCCCHHHHEEEEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHH
LNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
HHHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCEECCCCHHHHCCCCCCEEEEEECCC
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
AAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEF
CEEEEEECCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHCCCCEE
IITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVP
EEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECC
VGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINSGGPGMGIRVKLINNYMSIA
CCCCCCHHHHEEEEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHH
LNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
HHHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCEECCCCHHHHCCCCCCEEEEEECCC
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503 [H]