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The map label for this gene is mcrB [H]

Identifier: 157155277

GI number: 157155277

Start: 3346389

End: 3348446

Strand: Reverse

Name: mcrB [H]

Synonym: EcE24377A_3331

Alternate gene names: 157155277

Gene position: 3348446-3346389 (Counterclockwise)

Preceding gene: 157159116

Following gene: 157155806

Centisome position: 67.24

GC content: 44.7

Gene sequence:

>2058_bases
ATGGATCTTGTAGATAGTGTCGAAGCAGGCAAGCTGACGATCAGAGAATTGATTGATGCCCTGGTGAAAGATAAAAATTA
CACAGCTTCCAAGTGGGAGCAGCGATACCGGGAATTTACGACCTTGCTACAACAAACCTCAACTTTTGCTGAGCCTGAAA
CGGATGATTTAGTCAAGAGGCTCTGGTATGAACGTGATAACGGTATTGCCAGTATTCGTCAGGGCGTTCCATCATTAGCA
GAATATCAGCAAAGCCTCCCATTGCTTAGAGAACTCACCGAACGTGTTCGTCAACAACCAAATGAAGTTACCTACCAATA
CGTAGGCACTGCACTGCAGCAGGCCAAAAAGATCGGACAGCTTAAACGCATGTATTGGAGTTTAAGAAATCGTGTCTTTG
CCGCGTTCTCGCCAGAAAACTACACCAGTACTGTGGATGAGAATGCTTTCAATAAAACAGCAGAATTTCTAAATCAACGC
TTCCATCTCGGTTTGGTACTGACTGGAAATTGGTTACAGAAAAACTATGAATTGAAACAAGCCATACACGCCCAATCTCC
TGACACAGATCCCTATTACGTGAATATGGCTATCTGGCATATCTATGAATTGCTCCGTGAGCGCGATAATGAACAGAAGC
AAGAGAAAGTAGCAAGCAGTGAGCCCATCGAGAACAAGACTATTCCACATTCACCAACTAACGTGATCTTCTTTGGCCCT
CCAGGCACTGGCAAGACCTTCACGTTGCAGCAAAAAATGAAAGAGTATACCTCTCATGCAGTTCCTGCTGATCGTGAAGC
CTGGCTGGATTCTCGTCTTGAATCGTTGAACTGGATGCAGGTTATAACGCTGGTGCTGCTCGATCTTGGGAAACTAGCCA
AAGTTCGCCAAATTATTGAACATATGTGGTTTCAACGTAAGGCATTATTAAACGGTCGTAATGGCAATCTATCGAATACT
GCCTGGAAGGCTTTGCAAGCCTATACAATTCCCGAATCGTTAACTGTTGATTATAAAAATCGGCGTGAGCCTGCCGTATT
TGACAAAACGGATAACAGCGAATGGTTTCTAGTTGATTCACAGCTCGAGCAAGTGGAGGATTTGTTAGCGCTCTACGCCG
AACTTAAACGTGGTCCAAAATCTGCCGAAGCCATCCAGCGTTTTGCGGTTGTTACGTTCCACCAATCTTACGGCTATGAA
GAATTTATTGAAGGTATGCGTGCACGCTCTGACGAGAGTGGCAATATATCTTATCCCATTGAGCCGGGTATTTTTATGCG
CCTTTGCCAACGTGCTAATGCCGATCCAGCACATCGCTACGCCATTTTCATTGATGAGATCAATCGCGGAAACATATCCA
AGATCTTTGGAGAACTAATCTCACTCATTGAAGTAGACAAGCGTGCGGGTATGCCCAATGCAATGAGTCTGCAACTGTCT
TATAGCGGTGATTACTTTAGCGTGCCCGCCAATGTCGACATCATCGGAGCCATGAATACAGCAGACCGTTCTTTAGCTCT
GCTGGACACGGCTTTGCGCCGTCGCTTTGACTTTGTCGAAATGATGCCAGATCTTTCTTTACTGAGTGGAGCTAAGGTGA
AAGGCATAGAGCTTGAGTCGTTGTTAGAAAAACTCAATAGCCGCATCGAAGCTCTTTACGATCGTGAGCATACACTAGGA
CATGCATTCTTTATGCCGGTAAAAAATGCTCTCGATACCGGTGATGAAGAAACTGCGTTTAAACAATTAAAGATCGCATT
TCAGAAAAAGATCATTCCGCTTTTACAGGAATACTTTTTCGATGACTGGAACAAGATCCGGTTGGTGCTGGCAGACAATC
AAAAGCAAGACGACAACCAGCAATTCGTGATTGAGAAAACTGACGATCTCGATACGCTTTTTGGTAACAACCATGGTTTA
CGACGCCATGATCAGCAATCAACAACTTACGAGCTCAAAGATCTCGATCAAGGGGTCTGGAATATGCCAAAGGCTTATCG
TTCAATTTATCAGCCACAGCGGACCACTCCTGATGAGCAGGTCGTAAACCATGAGTGA

Upstream 100 bases:

>100_bases
ACTTATGGCGCTTGGCTACAATTGCATGCTTGGTAATATAGATTATAAAACAGCAGACTAGCTGCCTAGTAAGTAATGTA
ATAGCTGAGGAAATATCACG

Downstream 100 bases:

>100_bases
AGTTATCTCAATTTTTGAATATGACCTGTTGGGGAGTGGAAAAGCGGCTTCAGTTGGCGCAAAGTTGGTGCCTCAACAGG
TCTTTGATTATTTAGAAACA

Product: ATPase

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MDLVDSVEAGKLTIRELIDALVKDKNYTASKWEQRYREFTTLLQQTSTFAEPETDDLVKRLWYERDNGIASIRQGVPSLA
EYQQSLPLLRELTERVRQQPNEVTYQYVGTALQQAKKIGQLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKTAEFLNQR
FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHIYELLRERDNEQKQEKVASSEPIENKTIPHSPTNVIFFGP
PGTGKTFTLQQKMKEYTSHAVPADREAWLDSRLESLNWMQVITLVLLDLGKLAKVRQIIEHMWFQRKALLNGRNGNLSNT
AWKALQAYTIPESLTVDYKNRREPAVFDKTDNSEWFLVDSQLEQVEDLLALYAELKRGPKSAEAIQRFAVVTFHQSYGYE
EFIEGMRARSDESGNISYPIEPGIFMRLCQRANADPAHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLS
YSGDYFSVPANVDIIGAMNTADRSLALLDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLG
HAFFMPVKNALDTGDEETAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDDNQQFVIEKTDDLDTLFGNNHGL
RRHDQQSTTYELKDLDQGVWNMPKAYRSIYQPQRTTPDEQVVNHE

Sequences:

>Translated_685_residues
MDLVDSVEAGKLTIRELIDALVKDKNYTASKWEQRYREFTTLLQQTSTFAEPETDDLVKRLWYERDNGIASIRQGVPSLA
EYQQSLPLLRELTERVRQQPNEVTYQYVGTALQQAKKIGQLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKTAEFLNQR
FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHIYELLRERDNEQKQEKVASSEPIENKTIPHSPTNVIFFGP
PGTGKTFTLQQKMKEYTSHAVPADREAWLDSRLESLNWMQVITLVLLDLGKLAKVRQIIEHMWFQRKALLNGRNGNLSNT
AWKALQAYTIPESLTVDYKNRREPAVFDKTDNSEWFLVDSQLEQVEDLLALYAELKRGPKSAEAIQRFAVVTFHQSYGYE
EFIEGMRARSDESGNISYPIEPGIFMRLCQRANADPAHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLS
YSGDYFSVPANVDIIGAMNTADRSLALLDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLG
HAFFMPVKNALDTGDEETAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDDNQQFVIEKTDDLDTLFGNNHGL
RRHDQQSTTYELKDLDQGVWNMPKAYRSIYQPQRTTPDEQVVNHE
>Mature_685_residues
MDLVDSVEAGKLTIRELIDALVKDKNYTASKWEQRYREFTTLLQQTSTFAEPETDDLVKRLWYERDNGIASIRQGVPSLA
EYQQSLPLLRELTERVRQQPNEVTYQYVGTALQQAKKIGQLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKTAEFLNQR
FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHIYELLRERDNEQKQEKVASSEPIENKTIPHSPTNVIFFGP
PGTGKTFTLQQKMKEYTSHAVPADREAWLDSRLESLNWMQVITLVLLDLGKLAKVRQIIEHMWFQRKALLNGRNGNLSNT
AWKALQAYTIPESLTVDYKNRREPAVFDKTDNSEWFLVDSQLEQVEDLLALYAELKRGPKSAEAIQRFAVVTFHQSYGYE
EFIEGMRARSDESGNISYPIEPGIFMRLCQRANADPAHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLS
YSGDYFSVPANVDIIGAMNTADRSLALLDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLG
HAFFMPVKNALDTGDEETAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDDNQQFVIEKTDDLDTLFGNNHGL
RRHDQQSTTYELKDLDQGVWNMPKAYRSIYQPQRTTPDEQVVNHE

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=235, Percent_Identity=40, Blast_Score=151, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 79209; Mature: 79209

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLVDSVEAGKLTIRELIDALVKDKNYTASKWEQRYREFTTLLQQTSTFAEPETDDLVKR
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERVRQQPNEVTYQYVGTALQQAKKIGQ
HHHHCCCCHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LKRMYWSLRNRVFAAFSPENYTSTVDENAFNKTAEFLNQRFHLGLVLTGNWLQKNYELKQ
HHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHCHHEEEEEEECCHHHHHHHHHH
AIHAQSPDTDPYYVNMAIWHIYELLRERDNEQKQEKVASSEPIENKTIPHSPTNVIFFGP
HHHCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECC
PGTGKTFTLQQKMKEYTSHAVPADREAWLDSRLESLNWMQVITLVLLDLGKLAKVRQIIE
CCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMWFQRKALLNGRNGNLSNTAWKALQAYTIPESLTVDYKNRREPAVFDKTDNSEWFLVDS
HHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCEEECCCCCCCCCCEECCCCCCEEEECH
QLEQVEDLLALYAELKRGPKSAEAIQRFAVVTFHQSYGYEEFIEGMRARSDESGNISYPI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
EPGIFMRLCQRANADPAHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLS
CCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
YSGDYFSVPANVDIIGAMNTADRSLALLDTALRRRFDFVEMMPDLSLLSGAKVKGIELES
ECCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHH
LLEKLNSRIEALYDREHTLGHAFFMPVKNALDTGDEETAFKQLKIAFQKKIIPLLQEYFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DDWNKIRLVLADNQKQDDNQQFVIEKTDDLDTLFGNNHGLRRHDQQSTTYELKDLDQGVW
CCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHCHHH
NMPKAYRSIYQPQRTTPDEQVVNHE
CCCHHHHHHHCCCCCCCHHHHCCCC
>Mature Secondary Structure
MDLVDSVEAGKLTIRELIDALVKDKNYTASKWEQRYREFTTLLQQTSTFAEPETDDLVKR
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERVRQQPNEVTYQYVGTALQQAKKIGQ
HHHHCCCCHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LKRMYWSLRNRVFAAFSPENYTSTVDENAFNKTAEFLNQRFHLGLVLTGNWLQKNYELKQ
HHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHCHHEEEEEEECCHHHHHHHHHH
AIHAQSPDTDPYYVNMAIWHIYELLRERDNEQKQEKVASSEPIENKTIPHSPTNVIFFGP
HHHCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECC
PGTGKTFTLQQKMKEYTSHAVPADREAWLDSRLESLNWMQVITLVLLDLGKLAKVRQIIE
CCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMWFQRKALLNGRNGNLSNTAWKALQAYTIPESLTVDYKNRREPAVFDKTDNSEWFLVDS
HHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCEEECCCCCCCCCCEECCCCCCEEEECH
QLEQVEDLLALYAELKRGPKSAEAIQRFAVVTFHQSYGYEEFIEGMRARSDESGNISYPI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
EPGIFMRLCQRANADPAHRYAIFIDEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLS
CCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
YSGDYFSVPANVDIIGAMNTADRSLALLDTALRRRFDFVEMMPDLSLLSGAKVKGIELES
ECCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHH
LLEKLNSRIEALYDREHTLGHAFFMPVKNALDTGDEETAFKQLKIAFQKKIIPLLQEYFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DDWNKIRLVLADNQKQDDNQQFVIEKTDDLDTLFGNNHGLRRHDQQSTTYELKDLDQGVW
CCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHCHHH
NMPKAYRSIYQPQRTTPDEQVVNHE
CCCHHHHHHHCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]