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The map label for this gene is treB [H]

Identifier: 157163715

GI number: 157163715

Start: 4499974

End: 4501392

Strand: Reverse

Name: treB [H]

Synonym: EcHS_A4494

Alternate gene names: 157163715

Gene position: 4501392-4499974 (Counterclockwise)

Preceding gene: 157163716

Following gene: 157163714

Centisome position: 96.94

GC content: 54.62

Gene sequence:

>1419_bases
ATGAGCAAAATAAACCAAACGGATATCGATCGGTTGATTGAACTGGTCGGCGGGCGCGGCAATATTGCGACGGTGAGCCA
CTGTATTACTCGCCTGCGCTTTGTCCTCAACCAACCGGCCAATGCCAGACCGAAAGAAATTGAGCAACTCCCCATGGTGA
AAGGCTGTTTCACCAATGCCGGGCAATTTCAGGTGGTGATTGGCACCAACGTGGGTGATTACTATCAAGCACTAATAGCG
TCAACCGGACAGGCGCAGGTTGATAAAGAGCAGGTAAAAAAAGCCGCCCGGCAGAATATGAAATGGCATGAGCAGTTGAT
CTCTCATTTCGCGGAGATCTTCTTCCCGTTGCTGCCCGCGTTGATTAGCGGCGGTTTGATCCTCGGTTTTCGCAATGTGA
TCGGCGATTTGCCCATGAGCAACGGTCAAACGCTGGCGCAAATGTACCCTTCCCTGCAAACGATCTACGATTTTCTGTGG
TTGATCGGTGAAGCGATCTTCTTCTACCTGCCGGTCGGGATTTGCTGGTCAGCGGTGAAAAAAATGGGCGGCACGCCGAT
CCTTGGTATCGTGCTTGGCGTGACACTGGTTTCCCCCCAGCTGATGAACGCTTATCTGCTCGGGCAGCAGCTGCCGGAAG
TGTGGGACTTTGGCATGTTCAGCATCGCCAAAGTGGGCTATCAGGCGCAGGTGATCCCGGCACTGTTAGCCGGGCTGGCG
CTGGGCGTTATTGAAACTCGCCTTAAACGCATCGTACCGGATTACCTCTATCTGGTGGTGGTGCCCGTCTGTTCGCTGAT
CCTCGCGGTGTTCCTCGCCCATGCGCTGATTGGTCCGTTTGGTCGCATGATTGGCGATGGCGTTGCCTTTGCGGTACGTC
ACCTGATGACCGGCAGCTTTGCTCCGATTGGTGCGGCATTGTTTGGCTTCCTGTACGCGCCGCTGGTGATCACCGGCGTA
CACCAGACCACCCTTGCTATTGATTTGCAGATGATTCAAAGCATGGGTGGCACGCCAGTGTGGCCGCTGATTGCGCTGTC
GAATATCGCTCAGGGCTCCGCCGTGATAGGCATTATCATTTCCAGCCGCAAGCACAATGAACGCGAGATCTCCGTGCCTG
CCGCTATCTCCGCCTGGCTTGGGGTCACTGAGCCTGCAATGTACGGCATCAACCTGAAATATCGCTTCCCGATGCTGTGC
GCGATGATTGGTTCTGGTCTGGCAGGATTACTATGCGGCCTGAACGGCGTTATGGCGAATGGTATCGGCGTAGGCGGCCT
GCCGGGAATTCTCTCGATTCAACCGAGCTACTGGCAGGTATTTGCGCTGGCAATGGTTATCGCCATCATCATCCCGATTG
TACTCACCTCGTTTATCTATCAGCGGAAATACCGCCTGGGCACGCTGGATATTGTTTAA

Upstream 100 bases:

>100_bases
GTGATCTTCGCTGCGTTTCGGGAACGTTCCCGTTTTTAAATTTTTCCGCGCAATATATTCTGCAGCCAACCAAAAATGTC
ATCTGCCATGGGGCTTTATG

Downstream 100 bases:

>100_bases
TTTTCTTCGGGGCGCAATTGCGCCCCCTCGCATTTGCAGGAATAACGTAATGACTAATCTTCCCCACTGGTGGCAAAACG
GCGTTATCTACCAGATTTAT

Product: trehalose(maltose)-specific PTS system components IIBC

Products: NA

Alternate protein names: EIIBC-Tre; EII-Tre; Trehalose-specific phosphotransferase enzyme IIB component; PTS system trehalose-specific EIIB component; Trehalose permease IIC component; PTS system trehalose-specific EIIC component [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANARPKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIA
STGQAQVDKEQVKKAARQNMKWHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFLW
LIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDFGMFSIAKVGYQAQVIPALLAGLA
LGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHALIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGV
HQTTLAIDLQMIQSMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGINLKYRFPMLC
AMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAIIIPIVLTSFIYQRKYRLGTLDIV

Sequences:

>Translated_472_residues
MSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANARPKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIA
STGQAQVDKEQVKKAARQNMKWHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFLW
LIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDFGMFSIAKVGYQAQVIPALLAGLA
LGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHALIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGV
HQTTLAIDLQMIQSMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGINLKYRFPMLC
AMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAIIIPIVLTSFIYQRKYRLGTLDIV
>Mature_471_residues
SKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANARPKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIAS
TGQAQVDKEQVKKAARQNMKWHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFLWL
IGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDFGMFSIAKVGYQAQVIPALLAGLAL
GVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHALIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVH
QTTLAIDLQMIQSMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGINLKYRFPMLCA
MIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAIIIPIVLTSFIYQRKYRLGTLDIV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=472, Percent_Identity=99.364406779661, Blast_Score=942, Evalue=0.0,
Organism=Escherichia coli, GI1790159, Length=404, Percent_Identity=25.4950495049505, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788769, Length=426, Percent_Identity=26.7605633802817, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994906, Length=456, Percent_Identity=26.9736842105263, Blast_Score=132, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011296 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 50999; Mature: 50868

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANARPKEIEQLPMVKGCFTNA
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHCCCC
GQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMKWHEQLISHFAEIFFPLLPA
CCEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFLWLIGEAIFFYLPVGICWSAVK
HHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDFGMFSIAKVGYQAQVIPALLAGLA
HCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHH
LGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHALIGPFGRMIGDGVAFAVRHLMTGSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
APIGAALFGFLYAPLVITGVHQTTLAIDLQMIQSMGGTPVWPLIALSNIAQGSAVIGIII
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEE
SSRKHNEREISVPAAISAWLGVTEPAMYGINLKYRFPMLCAMIGSGLAGLLCGLNGVMAN
ECCCCCCCCCCCHHHHHHHHCCCCCHHCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHC
GIGVGGLPGILSIQPSYWQVFALAMVIAIIIPIVLTSFIYQRKYRLGTLDIV
CCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANARPKEIEQLPMVKGCFTNA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHCCCC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CCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608078; 7610040; 9278503 [H]