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The map label for this gene is treC [H]

Identifier: 157163714

GI number: 157163714

Start: 4498269

End: 4499924

Strand: Reverse

Name: treC [H]

Synonym: EcHS_A4493

Alternate gene names: 157163714

Gene position: 4499924-4498269 (Counterclockwise)

Preceding gene: 157163715

Following gene: 157163713

Centisome position: 96.91

GC content: 53.08

Gene sequence:

>1656_bases
ATGACTAATCTTCCCCACTGGTGGCAAAACGGCGTTATCTACCAGATTTATCCAAAGAGTTTTCAGGACACCACGGGTAG
CGGCACCGGCGATTTACGTGGCGTTATCCAACGCCTGGACTATCTGCATAAACTGGGCGTTGATGCCATCTGGCTAACCC
CCTTTTATATCTCTCCCCAGGTCGATAACGGTTACGACGTAGCGAACTATACGGCGATTGATCCCATCTACGGCACGCTG
GACGATTTTGACGAACTGGTGACGCAGGCAAAATCGCGCGGGATTCGTATCATTCTCGATATGGTGTTTAACCATACCTC
TACCCAACATGCCTGGTTTCGCGAGGCGCTGAACAAAGAAAGCCCTTACCGCCAGTTTTATATCTGGCGCGATGGCGAAC
CAGAAACGCCACCGAACAACTGGCGTTCAAAATTTGGCGGTAGTGCGTGGCGCTGGCATGCGGAAAGCGAACAGTACTAT
TTGCATCTCTTTGCACCAGAACAGGCGGATCTCAACTGGGAGAATCCAGCGGTACGCGCAGAGCTGAAAAAAGTCTGTGA
ATTCTGGGCCGATCGTGGGGTCGACGGGTTGCGCCTGGATGTGGTGAATCTGATCTCCAAAGACCCGCGTTTCCCTGAAG
ACCTGGATGGCGACGGGCGTCGCTTCTACACCGACGGGCCACGAGCACACGAGTTTTTGCACGAGATGAACCGCGATGTG
TTTACGCCACGCGGGTTAATGACCGTAGGTGAAATGTCCTCCACCAGCCTTGAGCATTGCCAGCGATACGCGGCACTGAC
AGGCAGTGAATTGTCGATGACCTTTAATTTTCATCACCTGAAGGTCGATTATCCCGGTGGTGAAAAATGGACGCTGGCTA
AACCTGACTTTGTGGCGTTGAAAACATTGTTCCGCCACTGGCAACAAGGAATGCACAACGTGGCATGGAATGCCTTGTTC
TGGTGTAACCACGATCAGCCGCGCATTGTTTCTCGCTTTGGTGATGAAGGTGAATACCGCGTGCCTGCGGCAAAAATGCT
GGCGATGGTGCTGCATGGCATGCAGGGAACGCCGTATATCTACCAGGGCGAAGAGATTGGCATGACCAACCCGCATTTCA
CGCGCATTACTGACTATCGCGACGTGGAGAGCCTCAATATGTTTGCCGAGCTGCGCAACGATGGTCGTGATGCCGACGAG
TTATTGGCAATCCTCGCCAGTAAATCCCGTGACAACAGCCGCACGCCCATGCAATGGAGCAACGGCGATAATGCCGGGTT
TACGGCTGGCGAACCGTGGATTGGCCTGGGCGATAACTATCAACAAATCAACGTAGAAGCCGCGCTGGCCGATGAGTCCT
CGGTGTTTTACACCTACCAAAAGTTAATCGCACTGCGTAAGCAAGAAGCCATCCTGACATGGGGCAATTACCAGGATCTG
CTGCCAAACAGCCCTGTATTGTGGTGCTATCGCCGTGAATGGAAGGGGCAAACCTTGCTGGTCATTGCCAACCTTAGCCG
TGAGATCCAACCCTGGCAGCCAGGGCAAATGCGCGGCAACTGGCAGCTTGTGATGCATAACTACGAAGAAGCCTCACCAC
AACCCTGTGCCATGAATTTACGGCCTTTTGAGGCTGTCTGGTGGTTACAGAAGTAA

Upstream 100 bases:

>100_bases
TCGTTTATCTATCAGCGGAAATACCGCCTGGGCACGCTGGATATTGTTTAATTTTCTTCGGGGCGCAATTGCGCCCCCTC
GCATTTGCAGGAATAACGTA

Downstream 100 bases:

>100_bases
ATCTTCCCTAAGCCCCGGTAATGCCGGTCATTCCGGGGTTTTGTCTGTCAGCATGTTGTTTTTGTTGATTTTTCAACCAG
CAAATTCATTAAAAAATTTA

Product: trehalose-6-phosphate hydrolase

Products: NA

Alternate protein names: Alpha,alpha-phosphotrehalase [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTL
DDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDV
FTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALF
WCNHDQPRIVSRFGDEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADE
LLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDL
LPNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK

Sequences:

>Translated_551_residues
MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTL
DDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDV
FTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALF
WCNHDQPRIVSRFGDEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADE
LLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDL
LPNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK
>Mature_550_residues
TNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLD
DFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYYL
HLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDVF
TPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFW
CNHDQPRIVSRFGDEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADEL
LAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDLL
PNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=522, Percent_Identity=29.6934865900383, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI65506891, Length=108, Percent_Identity=36.1111111111111, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI61744481, Length=108, Percent_Identity=36.1111111111111, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI61744475, Length=107, Percent_Identity=36.4485981308411, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI61744479, Length=108, Percent_Identity=36.1111111111111, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI61744483, Length=108, Percent_Identity=36.1111111111111, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI61744477, Length=107, Percent_Identity=36.4485981308411, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790687, Length=551, Percent_Identity=98.9110707803993, Blast_Score=1144, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=188, Percent_Identity=31.3829787234043, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25147709, Length=488, Percent_Identity=26.4344262295082, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32565753, Length=527, Percent_Identity=22.9601518026565, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322245, Length=578, Percent_Identity=36.159169550173, Blast_Score=353, Evalue=4e-98,
Organism=Saccharomyces cerevisiae, GI6321726, Length=576, Percent_Identity=35.7638888888889, Blast_Score=352, Evalue=8e-98,
Organism=Saccharomyces cerevisiae, GI6324416, Length=576, Percent_Identity=36.1111111111111, Blast_Score=340, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6322241, Length=576, Percent_Identity=36.1111111111111, Blast_Score=339, Evalue=5e-94,
Organism=Saccharomyces cerevisiae, GI6322021, Length=576, Percent_Identity=36.1111111111111, Blast_Score=339, Evalue=5e-94,
Organism=Saccharomyces cerevisiae, GI6321731, Length=586, Percent_Identity=34.4709897610922, Blast_Score=327, Evalue=4e-90,
Organism=Saccharomyces cerevisiae, GI6319776, Length=586, Percent_Identity=34.3003412969283, Blast_Score=325, Evalue=8e-90,
Organism=Drosophila melanogaster, GI24583745, Length=537, Percent_Identity=35.9404096834264, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI221330053, Length=499, Percent_Identity=37.2745490981964, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24583747, Length=545, Percent_Identity=36.1467889908257, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24583749, Length=545, Percent_Identity=36.1467889908257, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24586597, Length=532, Percent_Identity=34.3984962406015, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24586599, Length=578, Percent_Identity=31.8339100346021, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24586587, Length=503, Percent_Identity=33.5984095427435, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24586593, Length=520, Percent_Identity=32.6923076923077, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI45549022, Length=529, Percent_Identity=33.2703213610586, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24586589, Length=536, Percent_Identity=33.3955223880597, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24586591, Length=494, Percent_Identity=32.9959514170041, Blast_Score=254, Evalue=2e-67,
Organism=Drosophila melanogaster, GI281360393, Length=466, Percent_Identity=30.4721030042918, Blast_Score=201, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR012769 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.93 [H]

Molecular weight: Translated: 63900; Mature: 63769

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQ
CCCCCCHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
VDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKE
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEHHHCCCCCHHHHHHHHHCCC
SPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYYLHLFAPEQADLNWENPAVRA
CCCEEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCHHHH
ELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDV
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCC
FTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVAL
CCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEECCCCHHHH
KTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFGDEGEYRVPAAKMLAMVLHGMQGTPYI
HHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCEE
YQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADELLAILASKSRDNSRTPMQWS
EECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEC
NGDNAGFTAGEPWIGLGDNYQQINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDL
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCHHH
LPNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNL
CCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
RPFEAVWWLQK
CCCCCEEEECC
>Mature Secondary Structure 
TNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHKLGVDAIWLTPFYISPQ
CCCCCHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
VDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKE
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEHHHCCCCCHHHHHHHHHCCC
SPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYYLHLFAPEQADLNWENPAVRA
CCCEEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCHHHH
ELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDV
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCC
FTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVAL
CCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEECCCCHHHH
KTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFGDEGEYRVPAAKMLAMVLHGMQGTPYI
HHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCEE
YQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADELLAILASKSRDNSRTPMQWS
EECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEC
NGDNAGFTAGEPWIGLGDNYQQINVEAALADESSVFYTYQKLIALRKQEAILTWGNYQDL
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCHHH
LPNSPVLWCYRREWKGQTLLVIANLSREIQPWQPGQMRGNWQLVMHNYEEASPQPCAMNL
CCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
RPFEAVWWLQK
CCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8083158; 7610040; 9278503; 8421692 [H]